Publication date: Available online 14 December 2017 Source:Structure
Author(s): Lindsay A. Baker, Tessa Sinnige, Pascale Schellenberger, Jeanine de Keyzer, C. Alistair Siebert, Arnold J.M. Driessen, Marc Baldus, Kay Grünewald
Membrane proteins remain challenging targets for structural biology, despite much effort, as their native environment is heterogeneous and complex. Most methods rely on detergents to extract membrane proteins from their native environment, but this removal can significantly alter the structure and function of these proteins. Here, we overcome these challenges with a hybrid method to study membrane proteins in their native membranes, combining high-resolution solid-state nuclear magnetic resonance spectroscopy and electron cryotomography using the same sample. Our method allows the structure and function of membrane proteins to be studied in their native environments, across different spatial and temporal resolutions, and the combination is more powerful than each technique individually. We use the method to demonstrate that the bacterial membrane protein YidC adopts a different conformation in native membranes and that substrate binding to YidC in these native membranes differs from purified and reconstituted systems. Graphical abstract
Teaser
Membrane proteins are often treated with detergents, which can affect structure and activity. Baker et*al. apply a hybrid method to bacterial membrane proteins in native membranes without detergent, using solid-state NMR spectroscopy and electron cryotomography. They find that the structure and function of YidC differ with and without detergent.
[NMR paper] Proton-Detected NMR Spectroscopy of Nanodisc-Embedded Membrane Proteins: MAS Solid-State vs. Solution-State Methods.
Proton-Detected NMR Spectroscopy of Nanodisc-Embedded Membrane Proteins: MAS Solid-State vs. Solution-State Methods.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Proton-Detected NMR Spectroscopy of Nanodisc-Embedded Membrane Proteins: MAS Solid-State vs. Solution-State Methods.
J Phys Chem B. 2017 Jul 24;:
Authors: Lakomek NA, Frey L, Bibow S, Böckmann A, Riek R, Meier BH
Abstract
The structural and dynamical characterization of membrane proteins...
nmrlearner
Journal club
0
07-25-2017 07:46 PM
Journal Highlight: Membrane proteins in their native habitat as seen by solid-state NMR spectroscopy
Journal Highlight: Membrane proteins in their native habitat as seen by solid-state NMR spectroscopy
http://www.spectroscopynow.com/common/images/thumbnails/14fff98e0e1.jpgRecent progress in the solid-state NMR spectroscopy of membrane proteins within a cellular membrane has been reviewed, along with requirements for sample preparation.
Read the rest at Spectroscopynow.com
nmrlearner
General
0
09-28-2015 06:31 PM
Membrane proteins in their native habitat as seen by solid-state NMR spectroscopy
Membrane proteins in their native habitat as seen by solid-state NMR spectroscopy
Abstract
Membrane proteins play many critical roles in cells, mediating flow of material and information across cell membranes. They have evolved to perform these functions in the environment of a cell membrane, whose physicochemical properties are often different from those of common cell membrane mimetics used for structure determination. As a result, membrane proteins are difficult to study by traditional methods of structural biology, and they are significantly underrepresented in the protein structure...
nmrlearner
Journal club
0
05-27-2015 10:39 AM
[NMR paper] Membrane proteins in their native habitat as seen by solid-state NMR spectroscopy.
Membrane proteins in their native habitat as seen by solid-state NMR spectroscopy.
Related Articles Membrane proteins in their native habitat as seen by solid-state NMR spectroscopy.
Protein Sci. 2015 May 13;
Authors: Brown LS, Ladizhansky V
Abstract
Membrane proteins play many critical roles in cells, mediating flow of material and information across cell membranes. They have evolved to perform these functions in the environment of a cell membrane, whose physicochemical properties are often different from those of common cell...
nmrlearner
Journal club
0
05-15-2015 08:02 PM
Membrane proteins in their native habitat as seen by solid-state NMR spectroscopy
Membrane proteins in their native habitat as seen by solid-state NMR spectroscopy
Abstract
Membrane proteins play many critical roles in cells, mediating flow of material and information across cell membranes. They have evolved to perform these functions in the environment of a cell membrane, whose physicochemical properties are often different from those of common cell membrane mimetics used for structure determination. As a result, membrane proteins are difficult to study by traditional methods of structural biology, and they are significantly underrepresented in the protein...
nmrlearner
Journal club
0
05-13-2015 02:01 PM
[NMR paper] Structure Determination of Membrane Proteins in Their Native Phospholipid Bilayer Environment by Rotationally Aligned Solid-State NMR Spectroscopy.
Structure Determination of Membrane Proteins in Their Native Phospholipid Bilayer Environment by Rotationally Aligned Solid-State NMR Spectroscopy.
Structure Determination of Membrane Proteins in Their Native Phospholipid Bilayer Environment by Rotationally Aligned Solid-State NMR Spectroscopy.
Acc Chem Res. 2013 Jul 5;
Authors: Opella SJ
Abstract
One of the most important topics in experimental structural biology is determining the structures of membrane proteins. These structures represent one-third of all of the information...
nmrlearner
Journal club
0
07-09-2013 02:47 PM
Proton-Detected Solid-State NMR Spectroscopy of Fibrillar and Membrane Proteins.
Proton-Detected Solid-State NMR Spectroscopy of Fibrillar and Membrane Proteins.
Proton-Detected Solid-State NMR Spectroscopy of Fibrillar and Membrane Proteins.
Angew Chem Int Ed Engl. 2011 Apr 20;
Authors: Linser R, Dasari M, Hiller M, Higman V, Fink U, Lopez Del Amo JM, Markovic S, Handel L, Kessler B, Schmieder P, Oesterhelt D, Oschkinat H, Reif B
nmrlearner
Journal club
0
04-22-2011 02:00 PM
Proton-Detected Solid-State NMR Spectroscopy of Fibrillar and Membrane Proteins.
Proton-Detected Solid-State NMR Spectroscopy of Fibrillar and Membrane Proteins.
Proton-Detected Solid-State NMR Spectroscopy of Fibrillar and Membrane Proteins.
Angew Chem Int Ed Engl. 2011 Apr 14;
Authors: Linser R, Dasari M, Hiller M, Higman V, Fink U, Lopez Del Amo JM, Markovic S, Handel L, Kessler B, Schmieder P, Oesterhelt D, Oschkinat H, Reif B