NMR paper Circular dichroic and 1H-NMR studies on the aged form of bovine plasma albumin.

		BioNMR > Educational resources > Journal club
[NMR paper] Circular dichroic and 1H-NMR studies on the aged form of bovine plasma albumin.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-21-2010, 11:12 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Circular dichroic and 1H-NMR studies on the aged form of bovine plasma albumin.

Circular dichroic and 1H-NMR studies on the aged form of bovine plasma albumin.

Related Articles Circular dichroic and 1H-NMR studies on the aged form of bovine plasma albumin.

Int J Pept Protein Res. 1991 Sep;38(3):260-6

Authors: Era S, Kuwata K, Sogami M, Kato K, Watari H

Bovine plasma albumin (BPA) has 17 disulfide bonds and approximately one SH group at Cys-34 which catalyzes the intramolecular SH, S-S exchange reaction in the alkaline region at low ionic strength, resulting in the formation of the aged form (A-form). 1) Fractions of alpha-helix (f alpha) and beta-form (f beta) of iodoacetamide-blocked non-aged BPA (IA-BPA) at pH 6.5 (the N-form) and 9.0 (the B-form) in the absence of added salt were 0.70, 0.12 and 0.62, 0.18, respectively (Era et al. (1990]. However, there were no changes in f alpha and f beta of the iodoacetamide-blocked A-form (IA-A-form) over the pH range from 5.5 to 9.1 in the absence of added salt or in 0.10 M KCl (f alpha approximately 0.60, f beta approximately 0.20), indicating that the secondary structure of the IA-A-form might be similar to that of non-aged IA-BPA at pH 9.0 (B-form) in the absence of added salt, that is, the frozen B-form, stabilized covalently by the repairing of disulfide bonds. 2) The rigidity of the A- and IA-A-forms, as monitored by cross-relaxation times between irradiated and observed protein protons, was similar to or slightly higher than that of non-aged IA-BPA or BMA.(ABSTRACT TRUNCATED AT 250 WORDS)

PMID: 1761372 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
(31)P NMR and AFM studies on the destabilization of cell and model membranes by the major bovine seminal plasma protein, PDC-109. (31)P NMR and AFM studies on the destabilization of cell and model membranes by the major bovine seminal plasma protein, PDC-109. Related Articles (31)P NMR and AFM studies on the destabilization of cell and model membranes by the major bovine seminal plasma protein, PDC-109. IUBMB Life. 2010 Nov;62(11):841-51 Authors: Damai RS, Sankhala RS, Anbazhagan V, Swamy MJ The effect of PDC-109 binding to dimyristoylphosphatidylcholine (DMPC) and dipalmitoylphosphatidylglycerol (DPPG) multilamellar vesicles (MLVs) and supported membranes was investigated by...	nmrlearner	Journal club	0	12-01-2010 04:41 PM
[NMR paper] The sterol carrier protein-2 fatty acid binding site: an NMR, circular dichroic, and The sterol carrier protein-2 fatty acid binding site: an NMR, circular dichroic, and fluorescence spectroscopic determination. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles The sterol carrier protein-2 fatty acid binding site: an NMR, circular dichroic, and fluorescence spectroscopic determination. Biochemistry. 1997 Feb 18;36(7):1719-29 Authors: Stolowich NJ, Frolov A, Atshaves B, Murphy EJ, Jolly CA, Billheimer JT, Scott AI, Schroeder F The interaction and orientation of fatty acids...	nmrlearner	Journal club	0	08-22-2010 03:31 PM
[NMR paper] The sterol carrier protein-2 fatty acid binding site: an NMR, circular dichroic, and The sterol carrier protein-2 fatty acid binding site: an NMR, circular dichroic, and fluorescence spectroscopic determination. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles The sterol carrier protein-2 fatty acid binding site: an NMR, circular dichroic, and fluorescence spectroscopic determination. Biochemistry. 1997 Feb 18;36(7):1719-29 Authors: Stolowich NJ, Frolov A, Atshaves B, Murphy EJ, Jolly CA, Billheimer JT, Scott AI, Schroeder F The interaction and orientation of fatty acids...	nmrlearner	Journal club	0	08-22-2010 03:03 PM
[NMR paper] Determination of molecular mobility of lyophilized bovine serum albumin and gamma-glo Determination of molecular mobility of lyophilized bovine serum albumin and gamma-globulin by solid-state 1H NMR and relation to aggregation-susceptibility. Related Articles Determination of molecular mobility of lyophilized bovine serum albumin and gamma-globulin by solid-state 1H NMR and relation to aggregation-susceptibility. Pharm Res. 1996 Jun;13(6):926-30 Authors: Yoshioka S, Aso Y, Kojima S PURPOSE: Feasibility of solid-state 1H NMR for determining the mobility of protein molecules in lyophilized cakes was considered. The mobility in...	nmrlearner	Journal club	0	08-22-2010 02:27 PM
[NMR paper] The structure of the O-linked carbohydrate chain of bovine seminal plasma protein PDC The structure of the O-linked carbohydrate chain of bovine seminal plasma protein PDC-109 revised by H-NMR spectroscopy A correction. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles The structure of the O-linked carbohydrate chain of bovine seminal plasma protein PDC-109 revised by H-NMR spectroscopy A correction. FEBS Lett. 1996 May 27;387(1):99-100 Authors: Gerwig GL, Calvete JJ, Töpfer-Petersen E, Vliegenthart JF	nmrlearner	Journal club	0	08-22-2010 02:27 PM
[NMR paper] Direct binding of ethanol to bovine serum albumin: a fluorescent and 13C NMR multiple Direct binding of ethanol to bovine serum albumin: a fluorescent and 13C NMR multiplet relaxation study. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles Direct binding of ethanol to bovine serum albumin: a fluorescent and 13C NMR multiplet relaxation study. Biochemistry. 1996 Jan 9;35(1):340-7 Authors: Avdulov NA, Chochina SV, Daragan VA, Schroeder F, Mayo KH, Wood WG Molecular mechanisms of ethanol interaction with proteins are not well-understood. In the present study, direct...	nmrlearner	Journal club	0	08-22-2010 02:27 PM
[NMR paper] The influence of hydration on the conformation of bovine serum albumin studied by sol The influence of hydration on the conformation of bovine serum albumin studied by solid-state 13C-NMR spectroscopy. Related Articles The influence of hydration on the conformation of bovine serum albumin studied by solid-state 13C-NMR spectroscopy. Biopolymers. 1993 Dec;33(12):1871-6 Authors: Gregory RB, Gangoda M, Gilpin RK, Su W 13C proton-decoupled cross-polarization magic-angle spinning nmr spectra of bovine serum albumin are reported as a function of hydration. Increases in hydration level enhance the resolution of the peak centered at...	nmrlearner	Journal club	0	08-22-2010 03:01 AM
[NMR paper] Circular dichroic and 1H-NMR studies on the aged form of bovine plasma albumin. Circular dichroic and 1H-NMR studies on the aged form of bovine plasma albumin. Related Articles Circular dichroic and 1H-NMR studies on the aged form of bovine plasma albumin. Int J Pept Protein Res. 1991 Sep;38(3):260-6 Authors: Era S, Kuwata K, Sogami M, Kato K, Watari H Bovine plasma albumin (BPA) has 17 disulfide bonds and approximately one SH group at Cys-34 which catalyzes the intramolecular SH, S-S exchange reaction in the alkaline region at low ionic strength, resulting in the formation of the aged form (A-form). 1) Fractions of...	nmrlearner	Journal club	0	08-21-2010 11:12 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off