[NMR paper] Spectral Comparisons of Mammalian Cells and Intact Organelles by Solid-State NMR.
Spectral Comparisons of Mammalian Cells and Intact Organelles by Solid-State NMR.
Related Articles Spectral Comparisons of Mammalian Cells and Intact Organelles by Solid-State NMR.
J Struct Biol. 2018 May 30;:
Authors: Werby SH, Cegelski L
Abstract
Whole-cell protein profiling, spatial localization, and quantification of activities such as gene transcription and protein translation are possible with modern biochemical and biophysical techniques. Yet, addressing questions of overall compositional changes within a cell - capturing...
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06-03-2018 01:03 PM
[NMR paper] A Genetically Encoded ?-Lactamase Reporter for Ultrasensitive (129) Xe NMR in Mammalian Cells.
A Genetically Encoded ?-Lactamase Reporter for Ultrasensitive (129) Xe NMR in Mammalian Cells.
Related Articles A Genetically Encoded ?-Lactamase Reporter for Ultrasensitive (129) Xe NMR in Mammalian Cells.
Angew Chem Int Ed Engl. 2016 Jun 15;
Authors: Wang Y, Roose BW, Palovcak EJ, Carnevale V, Dmochowski IJ
Abstract
Molecular imaging holds considerable promise for elucidating biological processes in normal physiology as well as disease states, but requires noninvasive methods for identifying analytes at sub-micromolar...
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06-16-2016 12:06 PM
[NMR paper] Characterization of proteins by in-cell NMR spectroscopy in cultured mammalian cells.
Characterization of proteins by in-cell NMR spectroscopy in cultured mammalian cells.
Characterization of proteins by in-cell NMR spectroscopy in cultured mammalian cells.
Nat Protoc. 2016 Jun;11(6):1101-1111
Authors: Barbieri L, Luchinat E, Banci L
Abstract
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05-20-2016 03:04 PM
[NMR paper] Algal autolysate medium to label proteins for NMR in mammalian cells.
Algal autolysate medium to label proteins for NMR in mammalian cells.
Related Articles Algal autolysate medium to label proteins for NMR in mammalian cells.
J Biomol NMR. 2016 Apr 22;
Authors: Fuccio C, Luchinat E, Barbieri L, Neri S, Fragai M
Abstract
In-cell NMR provides structural and functional information on proteins directly inside living cells. At present, the high costs of the labeled media for mammalian cells represent a limiting factor for the development of this methodology. Here we report a protocol to prepare a...
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04-24-2016 10:29 PM
Algal autolysate medium to label proteins for NMR in mammalian cells
Algal autolysate medium to label proteins for NMR in mammalian cells
Abstract
In-cell NMR provides structural and functional information on proteins directly inside living cells. At present, the high costs of the labeled media for mammalian cells represent a limiting factor for the development of this methodology. Here we report a protocol to prepare a homemade growth medium from Spirulina platensis autolysate, suitable to express uniformly labeled proteins inside mammalian cells at a reduced cost-per-sample. The human proteins SOD1 and Mia40 were...
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04-22-2016 08:45 PM
[NMR paper] Fluorine NMR-based assay in living mammalian cells.
Fluorine NMR-based assay in living mammalian cells.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Fluorine NMR-based assay in living mammalian cells.
Anal Biochem. 2015 Dec 11;
Authors: Veronesi M, Giacomina F, Romeo E, Castellani B, Ottonello G, Lambruschini C, Garau G, Scarpelli R, Bandiera T, Piomelli D, Dalvit C
Abstract
Nuclear Magnetic Resonance (NMR)-based screening has been recognized as a powerful approach for the identification...
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12-28-2015 12:26 AM
[NMR paper] Chemical proteomic tool for ligand mapping of CYP antitargets: an NMR-compatible 3D Q
Chemical proteomic tool for ligand mapping of CYP antitargets: an NMR-compatible 3D QSAR descriptor in the Heme-Based Coordinate System.
Related Articles Chemical proteomic tool for ligand mapping of CYP antitargets: an NMR-compatible 3D QSAR descriptor in the Heme-Based Coordinate System.
J Chem Inf Comput Sci. 2004 Jul-Aug;44(4):1456-65
Authors: Yao H, Costache AD, Sem DS
Chemical proteomic strategies strive to probe and understand protein-ligand interactions across gene families. One gene family of particular interest in drug and xenobiotic...