Characterizing Protein-Protein Interactions Using Solution NMR Spectroscopy.
Methods Mol Biol. 2018;1764:73-85
Authors: Ortega-Roldan JL, Blackledge M, Jensen MR
Abstract
In this chapter, we describe how NMR chemical shift titrations can be used to study the interaction between two proteins with emphasis on mapping the interface of the complex and determining the binding affinity from a quantitative analysis of the experimental data. In particular, we discuss the appearance of NMR spectra in different chemical exchange regimes (fast, intermediate, and slow) and how these regimes affect NMR data analysis.
[NMR paper] Investigating protein-ligand interactions by solution NMR spectroscopy.
Investigating protein-ligand interactions by solution NMR spectroscopy.
Investigating protein-ligand interactions by solution NMR spectroscopy.
Chemphyschem. 2018 Jan 04;:
Authors: Becker W, Bhattiprolu KC, Gubensäk N, Zangger K
Abstract
Protein-ligand interactions are of fundamental importance in almost all processes in living organisms. The ligands comprise small molecules, drugs or biological macromolecules and their interaction strength varies over several orders of magnitude. Solution NMR spectroscopy offers a large...
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01-10-2018 12:45 PM
[NMR paper] The study of transient protein-nanoparticle interactions by solution NMR spectroscopy.
The study of transient protein-nanoparticle interactions by solution NMR spectroscopy.
Related Articles The study of transient protein-nanoparticle interactions by solution NMR spectroscopy.
Biochim Biophys Acta. 2015 Apr 30;
Authors: Assfalg M, Ragona L, Pagano K, D'Onofrio M, Zanzoni S, Tomaselli S, Molinari H
Abstract
The rapid development of novel nanoscale materials for applications in biomedicine urges an improved characterization of the nano-bio interfaces. Nanoparticles exhibit unique structures and properties, often...
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05-06-2015 11:59 AM
The study of transient protein-nanoparticle interactions by solution NMR spectroscopy
The study of transient protein-nanoparticle interactions by solution NMR spectroscopy
Publication date: Available online 30 April 2015
Source:Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics</br>
Author(s): Michael Assfalg , Laura Ragona , Katiuscia Pagano , Mariapina D’Onofrio , Serena Zanzoni , Simona Tomaselli , Henriette Molinari</br>
The rapid development of novel nanoscale materials for applications in biomedicine urges an improved characterization of the nano-bio interfaces. Nanoparticles exhibit unique structures and properties, often...
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04-30-2015 09:13 PM
[NMR paper] Characterizing protein-glycosaminoglycan interactions using solution NMR spectroscopy.
Characterizing protein-glycosaminoglycan interactions using solution NMR spectroscopy.
Characterizing protein-glycosaminoglycan interactions using solution NMR spectroscopy.
Methods Mol Biol. 2015;1229:325-33
Authors: Joseph PR, Poluri KM, Sepuru KM, Rajarathnam K
Abstract
Solution nuclear magnetic resonance (NMR) spectroscopy and, in particular, chemical shift perturbation (CSP) titration experiments are ideally suited for characterizing the binding interface of macromolecular complexes. (1)H-(15) N-HSQC-based CSP studies have...
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10-19-2014 01:27 PM
[NMR paper] Characterizing carbohydrate-protein interactions by NMR.
Characterizing carbohydrate-protein interactions by NMR.
Characterizing carbohydrate-protein interactions by NMR.
Biopolymers. 2013 Jun 20;
Authors: Bewley CA, Shahzad-Ul-Hussan S
Abstract
Interactions between proteins and soluble carbohydrates and/or surface displayed glycans are central to countless recognition, attachment and signaling events in biology. The physical chemical features associated with these binding events vary considerably, depending on the biological system of interest. For example, carbohydrate-protein...
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06-21-2013 01:10 PM
[NMR paper] Characterizing RNA dynamics at atomic resolution using solution-state NMR spectroscopy.
From Mendeley Biomolecular NMR group:
Characterizing RNA dynamics at atomic resolution using solution-state NMR spectroscopy.
Nature methods (2011). Volume: 8, Issue: 11. Pages: 919-31. Jameson R Bothe, Evgenia N Nikolova, Catherine D Eichhorn, Jeetender Chugh, Alexandar L Hansen, Hashim M Al-Hashimi et al.
Many recently discovered noncoding RNAs do not fold into a single native conformation but sample many different conformations along their free-energy landscape to carry out their biological function. Here we review solution-state NMR techniques that measure the structural,...
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10-12-2012 09:58 AM
[NMR paper] Characterizing RNA dynamics at atomic resolution using solution-state NMR spectroscopy.
From Mendeley Biomolecular NMR group:
Characterizing RNA dynamics at atomic resolution using solution-state NMR spectroscopy.
Nature methods (2011). Volume: 8, Issue: 11. Pages: 919-31. Jameson R Bothe, Evgenia N Nikolova, Catherine D Eichhorn, Jeetender Chugh, Alexandar L Hansen, Hashim M Al-Hashimi et al.
Many recently discovered noncoding RNAs do not fold into a single native conformation but sample many different conformations along their free-energy landscape to carry out their biological function. Here we review solution-state NMR techniques that measure the structural,...
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08-24-2012 08:01 PM
Postdoctoral position to study Protein-Protein Interactions and their role in Mechanisms of Signal Transduction using protein solution NMR/x-ray crystallography
Spincore.com are advertising a postdoc NMR position. It sounds pretty interesting.
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Postdoctoral position to study Protein-Protein Interactions and their role in Mechanisms of Signal Transduction using protein solution NMR /x-ray crystallography
Case Medical School, Cleveland, Ohio, USA
How are signaling events transmitted from one protein to another? To answer this question we are looking to add a postdoctoral co-workers to our interdisciplinary team. Our interest is to understand protein-protein interactions, protein structure and dynamics in the context of cell signaling...
Characterizing Protein-Protein Interactions Using Solution NMR Spectroscopy.
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