Characterizing protein-glycosaminoglycan interactions using solution NMR spectroscopy.
Methods Mol Biol. 2015;1229:325-33
Authors: Joseph PR, Poluri KM, Sepuru KM, Rajarathnam K
Abstract
Solution nuclear magnetic resonance (NMR) spectroscopy and, in particular, chemical shift perturbation (CSP) titration experiments are ideally suited for characterizing the binding interface of macromolecular complexes. (1)H-(15) N-HSQC-based CSP studies have become the method of choice due to their simplicity, short time requirements, and not requiring high-level NMR expertise. Nevertheless, CSP studies for characterizing protein-glycosaminoglycan (GAG) interactions have been challenging due to binding-induced aggregation/precipitation and/or poor quality data. In this chapter, we discuss how optimizing experimental variables such as protein concentration, GAG size, and sensitivity of NMR instrumentation can overcome these roadblocks to obtain meaningful structural insights into protein-GAG interactions.
[NMR paper] Ca(2+) modulating ?-synuclein membrane transient interactions revealed by solution NMR spectroscopy.
Ca(2+) modulating ?-synuclein membrane transient interactions revealed by solution NMR spectroscopy.
Related Articles Ca(2+) modulating ?-synuclein membrane transient interactions revealed by solution NMR spectroscopy.
Biochim Biophys Acta. 2013 Dec 4;
Authors: Zhang Z, Dai C, Bai J, Xu G, Liu M, Li C
Abstract
?-synuclein is involved in Parkinson's disease and its interaction with cell membrane is crucial to its pathological and physiological functions. Membrane properties, such as curvature, lipid composition have been shown to affect the...
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12-10-2013 05:36 PM
[NMR paper] Characterizing carbohydrate-protein interactions by NMR.
Characterizing carbohydrate-protein interactions by NMR.
Characterizing carbohydrate-protein interactions by NMR.
Biopolymers. 2013 Jun 20;
Authors: Bewley CA, Shahzad-Ul-Hussan S
Abstract
Interactions between proteins and soluble carbohydrates and/or surface displayed glycans are central to countless recognition, attachment and signaling events in biology. The physical chemical features associated with these binding events vary considerably, depending on the biological system of interest. For example, carbohydrate-protein...
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06-21-2013 01:10 PM
[NMR paper] Characterizing RNA dynamics at atomic resolution using solution-state NMR spectroscopy.
From Mendeley Biomolecular NMR group:
Characterizing RNA dynamics at atomic resolution using solution-state NMR spectroscopy.
Nature methods (2011). Volume: 8, Issue: 11. Pages: 919-31. Jameson R Bothe, Evgenia N Nikolova, Catherine D Eichhorn, Jeetender Chugh, Alexandar L Hansen, Hashim M Al-Hashimi et al.
Many recently discovered noncoding RNAs do not fold into a single native conformation but sample many different conformations along their free-energy landscape to carry out their biological function. Here we review solution-state NMR techniques that measure the structural,...
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10-12-2012 09:58 AM
[NMR paper] Characterizing RNA dynamics at atomic resolution using solution-state NMR spectroscopy.
From Mendeley Biomolecular NMR group:
Characterizing RNA dynamics at atomic resolution using solution-state NMR spectroscopy.
Nature methods (2011). Volume: 8, Issue: 11. Pages: 919-31. Jameson R Bothe, Evgenia N Nikolova, Catherine D Eichhorn, Jeetender Chugh, Alexandar L Hansen, Hashim M Al-Hashimi et al.
Many recently discovered noncoding RNAs do not fold into a single native conformation but sample many different conformations along their free-energy landscape to carry out their biological function. Here we review solution-state NMR techniques that measure the structural,...
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08-24-2012 08:01 PM
Site-resolved measurement of water-protein interactions by solution NMR.
Site-resolved measurement of water-protein interactions by solution NMR.
Site-resolved measurement of water-protein interactions by solution NMR.
Nat Struct Mol Biol. 2011 Jan 2;
Authors: Nucci NV, Pometun MS, Wand AJ
The interactions of biological macromolecules with water are fundamental to their structure, dynamics and function. Historically, characterization of the location and residence times of hydration waters of proteins in solution has been quite difficult. Confining proteins within the nanoscale interior of a reverse micelle slows water...
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01-05-2011 09:51 PM
[NMR paper] High-pressure NMR spectroscopy for characterizing folding intermediates and denatured
High-pressure NMR spectroscopy for characterizing folding intermediates and denatured states of proteins.
Related Articles High-pressure NMR spectroscopy for characterizing folding intermediates and denatured states of proteins.
Methods. 2004 Sep;34(1):133-43
Authors: Kamatari YO, Kitahara R, Yamada H, Yokoyama S, Akasaka K
Extensive structural studies using high-pressure NMR spectroscopy have recently been carried out on proteins, which potentially contribute to our understanding of the mechanisms of protein folding. Pressure shifts the...
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11-24-2010 10:01 PM
[NMR paper] Identification of a novel glycosaminoglycan core-like molecule. I. 500 MHz 1H NMR ana
Identification of a novel glycosaminoglycan core-like molecule. I. 500 MHz 1H NMR analysis using a nano-NMR probe indicates the presence of a terminal alpha-GalNAc residue capping 4-methylumbelliferyl-beta-D-xylosides.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--highwire.stanford.edu-icons-externalservices-pubmed-standard-jbc_full_free.gif Related Articles Identification of a novel glycosaminoglycan core-like molecule. I. 500 MHz 1H NMR analysis using a nano-NMR probe indicates the presence of a terminal alpha-GalNAc residue capping...
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08-22-2010 03:41 AM
Postdoctoral position to study Protein-Protein Interactions and their role in Mechanisms of Signal Transduction using protein solution NMR/x-ray crystallography
Spincore.com are advertising a postdoc NMR position. It sounds pretty interesting.
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Postdoctoral position to study Protein-Protein Interactions and their role in Mechanisms of Signal Transduction using protein solution NMR /x-ray crystallography
Case Medical School, Cleveland, Ohio, USA
How are signaling events transmitted from one protein to another? To answer this question we are looking to add a postdoctoral co-workers to our interdisciplinary team. Our interest is to understand protein-protein interactions, protein structure and dynamics in the context of cell signaling...
Characterizing protein-glycosaminoglycan interactions using solution NMR spectroscopy.
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