NMR paper Characterizing the Diastereoisomeric Distribution of Phosphorothioate Oligonucleotides by Metal Ion Complexation Chromatography, In-Series Reversed Phase-Strong Anion Exchange Chromatography, and (31)P NMR

		BioNMR > Educational resources > Journal club
[NMR paper] Characterizing the Diastereoisomeric Distribution of Phosphorothioate Oligonucleotides by Metal Ion Complexation Chromatography, In-Series Reversed Phase-Strong Anion Exchange Chromatography, and (31)P NMR

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-24-2021, 06:01 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Characterizing the Diastereoisomeric Distribution of Phosphorothioate Oligonucleotides by Metal Ion Complexation Chromatography, In-Series Reversed Phase-Strong Anion Exchange Chromatography, and (31)P NMR

Replacement of a non-bridging oxygen atom of the phosphate diester linkage of an oligonucleotide by sulfur conveys pharmacokinetic benefits, such as increased nuclease resistance and enhanced protein binding. Substitution renders the internucleotide linkages chiral, and so phosphorothioate diester (PS) oligonucleotides comprise complex mixtures of diastereoisomers. Currently, chromatographic separation of individual diastereoisomers is limited to oligonucleotides that contain no more than about...

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Protein Crystallization and Crystallography Market Forecast to 2027 – Covid-19 Impact and Global Analysis – By Technology (Ion Exchange Chromatography, HPLC, Gel-Electrophoresis, NMR, X-Ray Crystallography); Products (Reagents, Instruments); End User Protein Crystallization and Crystallography Market Forecast to 2027 – Covid-19 Impact and Global Analysis – By Technology (Ion Exchange Chromatography, HPLC, Gel-Electrophoresis, NMR, X-Ray Crystallography); Products (Reagents, Instruments); End User (Pharmaceuticals & Biotechnology Companies) and Geography - Clark County Blog Protein Crystallization and Crystallography Market Forecast to 2027 – Covid-19 Impact and Global Analysis – By Technology (Ion Exchange Chromatography, HPLC, Gel-Electrophoresis, NMR, X-Ray Crystallography); Products (Reagents, Instruments); End User (Pharmaceuticals...	nmrlearner	Online News	0	05-01-2021 10:34 AM
Global Protein Crystallization & Crystallography (Ion-Exchange Chromatography ... - Marketwired (press release) Global Protein Crystallization & Crystallography (Ion-Exchange Chromatography ... - Marketwired (press release) <img alt="" height="1" width="1" /> Global Protein Crystallization & Crystallography (Ion-Exchange Chromatography ... Marketwired (press release) Protein crystallization & crystallography are techniques that are used very frequently by researchers to understand the three-dimensional structure of a protein. The market for protein crystallization & crystallography is expected to grow at a high ... and more » Read here	nmrlearner	Online News	0	07-10-2014 08:28 PM
Global Protein Crystallization & Crystallography (Ion-Exchange Chromatography ... - EIN News (press release) Global Protein Crystallization & Crystallography (Ion-Exchange Chromatography ... - EIN News (press release) <img alt="" height="1" width="1" /> Global Protein Crystallization & Crystallography (Ion-Exchange Chromatography ... EIN News (press release) Protein crystallization & crystallography are techniques that are used very frequently by researchers to understand the three-dimensional structure of a protein. The market for protein crystallization & crystallography is expected to grow at a high ... and more » Read here	nmrlearner	Online News	0	07-03-2014 06:13 PM
Protein Crystallization & Crystallography Market (Ion-Exchange Chromatography ... - WhaTech Protein Crystallization & Crystallography Market (Ion-Exchange Chromatography ... - WhaTech <img alt="" height="1" width="1" /> Protein Crystallization & Crystallography Market (Ion-Exchange Chromatography ... WhaTech RnRMarketResearch.com adds report â??Protein Crystallization & Crystallography Market by Technology (Ion-Exchange Chromatography, HPLC, Gel-Electrophoresis, NMR, X-Ray Crystallography), Products (Reagents & Instruments) & End-Users ... Read here	nmrlearner	Online News	0	06-06-2014 03:59 PM
[NMR paper] NMR screening of new carbocyanine dyes as ligands for affinity chromatography. NMR screening of new carbocyanine dyes as ligands for affinity chromatography. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary-Button_120x27px_FullText.gif Related Articles NMR screening of new carbocyanine dyes as ligands for affinity chromatography. J Mol Recognit. 2014 Apr;27(4):197-204 Authors: Cruz C, Boto RE, Drzazga AK, Almeida P, Queiroz JA Abstract Four new carbocyanines containing symmetric and asymmetric heterocyclic moieties and N-carboxyalkyl groups have...	nmrlearner	Journal club	0	03-05-2014 11:57 PM
Reversed-Phase LC Isolates Potent Insecticidal Protein From Tarantula - LCGC Chromatography Online Reversed-Phase LC Isolates Potent Insecticidal Protein From Tarantula - LCGC Chromatography Online http://www.bionmr.com//t3.gstatic.com/images?q=tbn:ANd9GcQPvzVNyrq21tcZWrk-F8daI_eTIZQf2JRjrGMp_N-ih8CFKL5y4R-wnmulIW0Ic3xWjeUk_Lq5 LCGC Chromatography Online <img alt="" height="1" width="1" /> Reversed-Phase LC Isolates Potent Insecticidal Protein From Tarantula LCGC Chromatography Online The structure of the peptide, was determined by nuclear magnetic resonance (NMR) spectroscopy and indicated high thermal and chemical stability. According to the paper, this suggests the potential to...	nmrlearner	Online News	0	10-03-2013 03:31 PM
[NMR tweet] Gas chromatography and nuclear magnetic resonance..yawns Gas chromatography and nuclear magnetic resonance..yawns Published by napturally_me (Brittney Lewis) on 2010-12-06T08:35:03Z Source: Twitter	nmrlearner	Twitter NMR	0	12-06-2010 09:10 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off