[NMR paper] Characterizing the Brownian Diffusion of Nanocolloids and Molecular Solutions: Diffusion Ordered NMR Spectroscopy versus Dynamic Light Scattering.
Related ArticlesCharacterizing the Brownian Diffusion of Nanocolloids and Molecular Solutions: Diffusion Ordered NMR Spectroscopy versus Dynamic Light Scattering.
J Phys Chem B. 2020 May 01;:
Authors: Zhang C, Jin Z, Zeng B, Wang W, Palui G, Mattoussi H
Abstract
Hydrodynamic size is a characteristic dimension that reflects the Brownian diffusion of objects, such as proteins, macromolecules and various colloids when dissolved/dispersed in fluid phases. This property is crucial when investigating the utility of colloidal nanocrystals and polymeric materials in biology. Dynamic light scattering (DLS) has been widely used to measure the diffusion coefficient and hydrodynamic size of such systems. Comparatively, Diffusion Ordered Spectroscopy (DOSY) is a relatively new analytical method that has provided researchers with an alternative experimental approach to access those parameters. Here, we apply DLS and DOSY-NMR spectroscopy simultaneously to characterize the diffusion coefficient and hydrodynamic size of several sets of nanocolloids, including dispersions of gold nanoparticles and luminescent quantum dots that are surface-capped with either hydrophobic or hydrophilic coatings, as well as a short oligomer and a low molecular weight polymer. We compare side-by-side the findings acquired from each measurement, which has allowed us to identify the benefits and constraints of each technique. Our results show that the two approaches provide comparable data when larger size nanocolloids are probed. However, we find that DOSY is substantially more effective in characterizing nanocolloids that are fluorescent and/or have very small dimension, as well as molecular scale organic ligands, where DLS reaches its limit. Additionally, we find that compared to DLS, DOSY tends to require higher solute concentrations and longer collection time in order to generate high signal-to-noise data.
PMID: 32356987 [PubMed - as supplied by publisher]
[NMR paper] 2D Diffusion-Ordered 1H-NMR Spectroscopy Lipidomic Profiling After Oral Single Macronutrient Loads: Influence of Obesity, Sex, and Female Androgen Excess.
2D Diffusion-Ordered 1H-NMR Spectroscopy Lipidomic Profiling After Oral Single Macronutrient Loads: Influence of Obesity, Sex, and Female Androgen Excess.
Related Articles 2D Diffusion-Ordered 1H-NMR Spectroscopy Lipidomic Profiling After Oral Single Macronutrient Loads: Influence of Obesity, Sex, and Female Androgen Excess.
Mol Nutr Food Res. 2020 Mar 18;:e1900928
Authors: Insenser M, Moncayo S, Martínez-García MÁ, Fernández-Durán E, Samino S, Álvarez-Blasco F, Luque-Ramírez M, Escobar-Morreale HF
Abstract
SCOPE: Postprandial...
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03-19-2020 06:10 PM
Single-scan 13C diffusion-ordered NMR spectroscopy of DNP-hyperpolarised substrates #DNPNMR
From The DNP-NMR Blog:
Single-scan 13C diffusion-ordered NMR spectroscopy of DNP-hyperpolarised substrates #DNPNMR
p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica}
Guduff, L., et al., Single-scan 13C diffusion-ordered NMR spectroscopy of DNP-hyperpolarised substrates. Chemistry, 2017: p. n/a-n/a.
https://www.ncbi.nlm.nih.gov/pubmed/28857281
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09-13-2017 08:48 PM
[NMR paper] Comparison of NMR and Dynamic Light Scattering for Measuring Diffusion Coefficients of Formulated Insulin: Implications for Particle Size Distribution*Measurements in Drug Products.
Comparison of NMR and Dynamic Light Scattering for Measuring Diffusion Coefficients of Formulated Insulin: Implications for Particle Size Distribution*Measurements in Drug Products.
Related Articles Comparison of NMR and Dynamic Light Scattering for Measuring Diffusion Coefficients of Formulated Insulin: Implications for Particle Size Distribution*Measurements in Drug Products.
AAPS J. 2017 Aug 08;:
Authors: Patil SM, Keire DA, Chen K
Abstract
Particle size distribution, a measurable physicochemical quantity, is a critical...
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08-10-2017 01:27 PM
[NMR paper] Liposcale: a novel advanced lipoprotein test based on 2D diffusion-ordered 1H NMR spectroscopy.
Liposcale: a novel advanced lipoprotein test based on 2D diffusion-ordered 1H NMR spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--highwire.stanford.edu-icons-externalservices-pubmed-standard-jlr_final.gif Related Articles Liposcale: a novel advanced lipoprotein test based on 2D diffusion-ordered 1H NMR spectroscopy.
J Lipid Res. 2015 Mar;56(3):737-46
Authors: Mallol R, Amigó N, Rodríguez MA, Heras M, Vinaixa M, Plana N, Rock E, Ribalta J, Yanes O, Masana L, Correig X
Abstract
Determination of...
[NMR paper] A new sequence for single-shot diffusion-weighted NMR spectroscopy by the trace of the diffusion tensor.
A new sequence for single-shot diffusion-weighted NMR spectroscopy by the trace of the diffusion tensor.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary-Button_120x27px_FullText.gif Related Articles A new sequence for single-shot diffusion-weighted NMR spectroscopy by the trace of the diffusion tensor.
Magn Reson Med. 2012 Dec;68(6):1705-12
Authors: Valette J, Giraudeau C, Marchadour C, Djemai B, Geffroy F, Ghaly MA, Le Bihan D, Hantraye P, Lebon V, Lethimonnier F
...
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05-31-2013 11:16 AM
[NMR paper] The hydration of proteins in solutions by self-diffusion coefficients. NMR study.
The hydration of proteins in solutions by self-diffusion coefficients. NMR study.
Related Articles The hydration of proteins in solutions by self-diffusion coefficients. NMR study.
Biochim Biophys Acta. 1996 Apr 17;1289(3):312-4
Authors: Baranowska HM, Olszewski KJ
The hydration of the globular (lysozyme, albumin) and fibrillar (fibrinogen) proteins in solution has been determined from the measurements of the self-diffusion coefficient by NMR pulsed gradient method. It has been concluded that the concentration dependencies of the...
Characterizing the Brownian Diffusion of Nanocolloids and Molecular Solutions: Diffusion Ordered NMR Spectroscopy versus Dynamic Light Scattering.
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