[NMR paper] Characterization of potential degradation products in a PEGylating reagent 20kDa monomethoxy polyethylene glycol propionaldehyde by RP-HPLC, APCI-MS and NMR.
Characterization of potential degradation products in a PEGylating reagent 20kDa monomethoxy polyethylene glycol propionaldehyde by RP-HPLC, APCI-MS and NMR.
Related ArticlesCharacterization of potential degradation products in a PEGylating reagent 20kDa monomethoxy polyethylene glycol propionaldehyde by RP-HPLC, APCI-MS and NMR.
J Pharm Biomed Anal. 2013 Nov 15;89C:221-226
Authors: Zhang H, Wilson J, Zhang J, Luo Y
Abstract
Ensuring quality of PEGylating reagents is essential for the successful development and manufacturing of PEGylated biopharmaceuticals. However, little is known about how to maintain and verify the quality of PEG raw materials for PEGylated protein manufacturing. In this study, monomethoxy polyethylene glycol propionaldehyde (mPEG-aldehyde) was subjected to conditions that mimic accelerated stability conditions. Separation of trace-level degradation products in the presence of mPEG-aldehyde was achieved by derivatization with 2,4-dinitrophenylhydrazine (DNPH), followed by reversed phase high performance liquid chromatography with ultraviolet detection (RP-HPLC-UV) at 355nm. Structural characterization by atmospheric pressure chemical ionization mass spectrometry (APCI-MS) identified formaldehyde, acetaldehyde, crotonaldehyde, acrolein, benzaldehyde, and tolualdehyde as major degradation products or process-related impurities. The presence of formaldehyde and acrolein was confirmed by (1)H NMR in the forced degraded mPEG-aldehyde samples without derivatization of mPEG-aldehyde. Findings from this study imply that reactive impurities could form as a result of inappropriate mPEG-aldehyde handling or storage. Further, a rapid screening method based on reversed phase HPLC was shown to be an effective screening assay used for routine screening of mPEG-aldehyde to ensure consistent PEGylated protein product quality.
PMID: 24316423 [PubMed - as supplied by publisher]
[NMR paper] (1)H NMR spectroscopic studies on the characterization of renal cell lines and identification of novel potential markers of in vitro nephrotoxicity.
(1)H NMR spectroscopic studies on the characterization of renal cell lines and identification of novel potential markers of in vitro nephrotoxicity.
Related Articles (1)H NMR spectroscopic studies on the characterization of renal cell lines and identification of novel potential markers of in vitro nephrotoxicity.
Biomarkers. 1996;1(1):35-43
Authors: Anthony ML, McDowell PC, Gray TJ, Blackmore M, Nicholson JK
Abstract
Abstract Cell cultures are increasingly used in the evaluation of chemically-induced nephrotoxicity. The utili of renal cell...
Magnetic resonance diffusion and relaxation characterization of water in the unfrozen vein network in polycrystalline ice and its response to microbial metabolic products
Magnetic resonance diffusion and relaxation characterization of water in the unfrozen vein network in polycrystalline ice and its response to microbial metabolic products
December 2012
Publication year: 2012
Source:Journal of Magnetic Resonance, Volume 225</br>
</br>
Polycrystalline ice, as found in glaciers and the ice sheets of Antarctica, is a low porosity porous media consisting of a complicated and dynamic pore structure of liquid-filled intercrystalline veins within a solid ice matrix. In this work, Nuclear Magnetic Resonance measurements of relaxation rates and...
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Magnetic resonance diffusion and relaxation characterization of water in the unfrozen vein network in polycrystalline ice and its response to microbial metabolic products
Magnetic resonance diffusion and relaxation characterization of water in the unfrozen vein network in polycrystalline ice and its response to microbial metabolic products
December 2012
Publication year: 2012
Source:Journal of Magnetic Resonance, Volume 225</br>
</br>
Polycrystalline ice, as found in glaciers and the ice sheets of Antarctica, is a low porosity porous media consisting of a complicated and dynamic pore structure of liquid-filled intercrystalline veins within a solid ice matrix. In this work, Nuclear Magnetic Resonance measurements of relaxation rates and...
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Magnetic Resonance Diffusion and Relaxation Characterization of Water in the Unfrozen Vein Network in Polycrystalline Ice and its Response to Microbial Metabolic Products
Magnetic Resonance Diffusion and Relaxation Characterization of Water in the Unfrozen Vein Network in Polycrystalline Ice and its Response to Microbial Metabolic Products
Publication year: 2012
Source:Journal of Magnetic Resonance</br>
Jennifer R. Brown, Timothy I. Brox, Sarah J. Vogt, Joseph D. Seymour, Mark Skidmore, Sarah L. Codd</br>
Polycrystalline ice, as found in glaciers and the ice sheets of Antarctica, is a low porosity porous media consisting of a complicated and dynamic pore structure of liquid-filled intercrystalline veins within a solid ice matrix. In...
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10-05-2012 09:10 AM
[NMR paper] Thermal stability studies of a globular protein in aqueous poly(ethylene glycol) by (
Thermal stability studies of a globular protein in aqueous poly(ethylene glycol) by (1)H NMR.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_120x27.gif Related Articles Thermal stability studies of a globular protein in aqueous poly(ethylene glycol) by (1)H NMR.
Biotechnol Bioeng. 1996 Aug 20;51(4):410-21
Authors: Hancock TJ, Hsu JT
The reversible folding destabilization of hen lysozyme has been confirmed by a melting temperature (T(m)) decrease in aqueous...
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[NMR paper] Co2+ as a shift reagent for 35Cl NMR of chloride with vesicles and cells.
Co2+ as a shift reagent for 35Cl NMR of chloride with vesicles and cells.
Related Articles Co2+ as a shift reagent for 35Cl NMR of chloride with vesicles and cells.
Biochemistry. 1992 Jul 14;31(27):6272-8
Authors: Shachar-Hill Y, Shulman RG
Applications of high-resolution 35Cl NMR to the study of chloride in vivo and in vesicles have hitherto been limited by problems of NMR detectability and of resolving internal from external signals. We have characterized the effects of Co2+ on the 35Cl resonance of Cl- in solution and have shown that when...
Characterization of potential degradation products in a PEGylating reagent 20kDa monomethoxy polyethylene glycol propionaldehyde by RP-HPLC, APCI-MS and NMR.
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