NMR paper Characterization of polyacrylamide-stabilized Pfl phage liquid crystals for protein N

		BioNMR > Educational resources > Journal club
[NMR paper] Characterization of polyacrylamide-stabilized Pfl phage liquid crystals for protein N

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-24-2010, 08:49 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Characterization of polyacrylamide-stabilized Pfl phage liquid crystals for protein N

Characterization of polyacrylamide-stabilized Pfl phage liquid crystals for protein NMR spectroscopy.

Related Articles Characterization of polyacrylamide-stabilized Pfl phage liquid crystals for protein NMR spectroscopy.

J Biomol NMR. 2002 Jan;22(1):83-7

Authors: Trempe JF, Morin FG, Xia Z, Marchessault RH, Gehring K

A new polymer-stabilized nematic liquid crystal has been characterized for the measurement of biomolecular residual dipolar couplings. Filamentous Pf1 phage were embedded in a polyacrylamide matrix that fixes the orientation of the particles. The alignment was characterized by the quadrupolar splitting of the 2H NMR water signal and by the measurement of 1H-15N residual dipolar couplings (RDC) in the archeal translation elongation factor 1beta. Protein dissolved in the polymer-stabilized medium orients quantitatively as in media without polyacrylamide. We show that the quadrupolar splitting and RDCs are zero in media in which the Pf1 phage particles are aligned at the magic angle. This allows measurement of J and dipolar couplings in a single sample.

PMID: 11885983 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR tweet] Nuclear Magnetic Resonance of Liquid Crystals (Partially Ordered Systems): http://amzn.to/m9kdPD Nuclear Magnetic Resonance of Liquid Crystals (Partially Ordered Systems): http://amzn.to/m9kdPD Published by Vanettahllv (Vanetta Geddis) on 2011-05-16T20:06:08Z Source: Twitter	nmrlearner	Twitter NMR	0	05-16-2011 08:11 PM
[NMR tweet] Nuclear Magnetic Resonance of Liquid Crystals (Partially Ordered Systems): http://amzn.to/fZJjl1 Nuclear Magnetic Resonance of Liquid Crystals (Partially Ordered Systems): http://amzn.to/fZJjl1 Published by Randiud18 (Randi Deblauw) on 2011-04-30T04:50:17Z Source: Twitter	nmrlearner	Twitter NMR	0	04-30-2011 05:47 AM
[NMR tweet] Nuclear Magnetic Resonance of Liquid Crystals (Partially Ordered Systems): http://amzn.to/fKahsB Nuclear Magnetic Resonance of Liquid Crystals (Partially Ordered Systems): http://amzn.to/fKahsB Published by Lahomawm62 (Lahoma Swagger) on 2011-04-28T13:06:51Z Source: Twitter	nmrlearner	Twitter NMR	0	04-28-2011 02:00 PM
[NMR tweet] Nuclear Magnetic Resonance of Liquid Crystals (Partially Ordered Systems): http://amzn.to/hBtMo4 Nuclear Magnetic Resonance of Liquid Crystals (Partially Ordered Systems): http://amzn.to/hBtMo4 Published by Marisafo66 (Marisa Jagodzinski) on 2011-04-25T21:47:25Z Source: Twitter	nmrlearner	Twitter NMR	0	04-25-2011 09:53 PM
[NMR tweet] Nuclear Magnetic Resonance of Liquid Crystals (Partially Ordered Systems): http://amzn.to/fZJjl1 Nuclear Magnetic Resonance of Liquid Crystals (Partially Ordered Systems): http://amzn.to/fZJjl1 Published by Lonifa40 (Loni Hackner) on 2011-04-23T17:48:40Z Source: Twitter	nmrlearner	Twitter NMR	0	04-23-2011 05:54 PM
[NMR tweet] Nuclear Magnetic Resonance of Liquid Crystals (Partially Ordered Systems): http://amzn.to/fKahsB Nuclear Magnetic Resonance of Liquid Crystals (Partially Ordered Systems): http://amzn.to/fKahsB Published by Brittneywz32 (Brittney Leland) on 2011-04-21T10:21:18Z Source: Twitter	nmrlearner	Twitter NMR	0	04-21-2011 10:30 AM
[NMR tweet] Nuclear Magnetic Resonance of Liquid Crystals (Partially Ordered Systems): http://amzn.to/fKahsB Nuclear Magnetic Resonance of Liquid Crystals (Partially Ordered Systems): http://amzn.to/fKahsB Published by Lovettahl25 (Lovetta Mentis) on 2011-04-20T15:22:33Z Source: Twitter	nmrlearner	Twitter NMR	0	04-20-2011 03:53 PM
[NMR paper] Bicelle-based liquid crystals for NMR-measurement of dipolar couplings at acidic and Bicelle-based liquid crystals for NMR-measurement of dipolar couplings at acidic and basic pH values. Related Articles Bicelle-based liquid crystals for NMR-measurement of dipolar couplings at acidic and basic pH values. J Biomol NMR. 1999 Feb;13(2):187-91 Authors: Ottiger M, Bax A It is demonstrated that mixtures of ditetradecyl-phosphatidylcholine or didodecyl-phoshatidylcholine and dihexyl-phosphatidylcholine in water from lyotropic liquid crystalline phases under similar conditions as previously reported for bicelles consisting of...	nmrlearner	Journal club	0	08-21-2010 04:03 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off