Characterization of membrane protein function by solid-state NMR spectroscopy.
Curr Opin Struct Biol. 2014 Apr 25;27C:48-55
Authors: Baker LA, Baldus M
Abstract
Membrane proteins are an important class of biological molecules whose association with lipid bilayers and intrinsic molecular mobility can complicate their structural study by high-resolution methods. As different experimental techniques require different membrane mimetics, it can be challenging to relate membrane protein structure to function. This review presents examples of the use of solid-state nuclear magnetic resonance spectroscopy (ssNMR) to correlate structure and function in membrane proteins with diverse biological roles, including signaling, transport, and enzymatic reactions. The types of ssNMR experiments, as well as sources of complementary information and implications for biology, will be discussed. An outlook towards extending ssNMR studies to cellular preparations will be given.
PMID: 24865155 [PubMed - as supplied by publisher]
Characterization of membrane protein function by solid-state NMR spectroscopy
Characterization of membrane protein function by solid-state NMR spectroscopy
Publication date: August 2014
Source:Current Opinion in Structural Biology, Volume 27</br>
Author(s): Lindsay A Baker , Marc Baldus</br>
Membrane proteins are an important class of biological molecules whose association with lipid bilayers and intrinsic molecular mobility can complicate their structural study by high-resolution methods. As different experimental techniques require different membrane mimetics, it can be challenging to relate membrane protein structure to function. This...
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[NMR paper] Solid state NMR: The essential technology for helical membrane protein structural characterization.
Solid state NMR: The essential technology for helical membrane protein structural characterization.
Related Articles Solid state NMR: The essential technology for helical membrane protein structural characterization.
J Magn Reson. 2013 Dec 19;
Authors: Cross TA, Ekanayake V, Paulino J, Wright A
Abstract
NMR spectroscopy of helical membrane proteins has been very challenging on multiple fronts. The expression and purification of these proteins while maintaining functionality has consumed countless graduate student hours. Sample...
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01-15-2014 05:16 PM
[NMR paper] Solid State NMR: The Essential Technology for Helical Membrane Protein Structural Characterization
Solid State NMR: The Essential Technology for Helical Membrane Protein Structural Characterization
Publication date: Available online 19 December 2013
Source:Journal of Magnetic Resonance</br>
Author(s): Timothy A. Cross , Vindana Ekanayake , Joana Paulino , Anna Wright</br>
NMR spectroscopy of helical membrane proteins has been very challenging on multiple fronts. The expression and purification of these proteins while maintaining functionality has consumed countless graduate student hours. Sample preparations have depended on whether solution or solid-state NMR...
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12-19-2013 06:38 PM
Expression, Purification, and Solid-State NMR Characterization of the Membrane Binding Heme Protein Nitrophorin 7 in Two Electronic Spin States
Expression, Purification, and Solid-State NMR Characterization of the Membrane Binding Heme Protein Nitrophorin 7 in Two Electronic Spin States
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/bi401020t/aop/images/medium/bi-2013-01020t_0007.gif
Biochemistry
DOI: 10.1021/bi401020t
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09-27-2013 03:28 AM
[NMR paper] Expression, Purification and Solid-state NMR Characterization of the Membrane Binding Heme Protein Nitrophorin 7 in two Electronic Spin States.
Expression, Purification and Solid-state NMR Characterization of the Membrane Binding Heme Protein Nitrophorin 7 in two Electronic Spin States.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Expression, Purification and Solid-state NMR Characterization of the Membrane Binding Heme Protein Nitrophorin 7 in two Electronic Spin States.
Biochemistry. 2013 Sep 13;
Authors: Varghese S, Yang F, Pacheco V, Wrede K, Medvedev A, Ogata H, Knipp M, Heise H
Abstract
The...
Recent contributions from solid-state NMR to the understanding of membrane protein structure and function.
Recent contributions from solid-state NMR to the understanding of membrane protein structure and function.
Recent contributions from solid-state NMR to the understanding of membrane protein structure and function.
Curr Opin Chem Biol. 2011 Aug 19;
Authors: Judge PJ, Watts A
Abstract
The plasma membrane functions as a semi-permeable barrier, defining the interior (or cytoplasm) of an individual cell. This highly dynamic and complex macromolecular assembly comprises predominantly lipids and proteins held together by entropic forces and...
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08-25-2011 04:10 PM
Solid-State NMR Spectroscopy of Membrane-Associated Myelin Basic Protein-Conformation
Solid-State NMR Spectroscopy of Membrane-Associated Myelin Basic Protein-Conformation and Dynamics of an Immunodominant Epitope.
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Biophys J. 2010 Aug 9;99(4):1247-1255
Authors: Ahmed MA, Bamm VV, Harauz G, Ladizhansky V
Myelin basic protein (MBP) maintains the tight multilamellar compaction of the myelin sheath in the central nervous system through peripheral binding of adjacent lipid bilayers of...
Characterization of membrane protein function by solid-state NMR spectroscopy.
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