The variety of magnetic properties exhibited by paramagnetic lanthanoids provides outstanding information in NMR-based structural biology and therefore can be a very useful tool for characterizing lanthanoid-binding proteins. Because of their dependence on the relative positions of the protein nuclei and of the lanthanoid ion, the paramagnetic restraints (PCS, PRDC and PRE) provide information on structure and dynamics of proteins. In this Chapter, we cover the use of lanthanoids in structural...
Generation and Characterization of Recombinant Antibody-likeADP-Ribose Binding Proteins
Generation and Characterization of Recombinant Antibody-likeADP-Ribose Binding Proteins
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Biochemistry
DOI: 10.1021/acs.biochem.7b00670
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11-23-2017 08:38 AM
Lanthanoid tagging via an unnatural amino acid for protein structure characterization
Lanthanoid tagging via an unnatural amino acid for protein structure characterization
Abstract
Lanthanoid pseudo-contact shift (PCS) provides long-range structural information between a paramagnetic tag and protein nuclei. However, for proteins with native cysteines, site-specific attachment may only utilize functional groups orthogonal to sulfhydryl chemistry. Here we report two lanthanoid probes, DTTA-C3-yne and DTTA-C4-yne, which can be conjugated to an unnatural amino acid pAzF in the target protein via azide-alkyne cycloaddition. Demonstrated...
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04-01-2017 04:09 PM
[NMR paper] A new carbamidemethyl-linked lanthanoid chelating tag for PCS NMR spectroscopy of proteins in living HeLa cells.
A new carbamidemethyl-linked lanthanoid chelating tag for PCS NMR spectroscopy of proteins in living HeLa cells.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles A new carbamidemethyl-linked lanthanoid chelating tag for PCS NMR spectroscopy of proteins in living HeLa cells.
J Biomol NMR. 2016 Sep 8;
Authors: Hikone Y, Hirai G, Mishima M, Inomata K, Ikeya T, Arai S, Shirakawa M, Sodeoka M, Ito Y
Abstract
Structural analyses of...
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09-22-2016 06:31 AM
A new carbamidemethyl-linked lanthanoid chelating tag for PCS NMR spectroscopy of proteins in living HeLa cells
A new carbamidemethyl-linked lanthanoid chelating tag for PCS NMR spectroscopy of proteins in living HeLa cells
Abstract
Structural analyses of proteins under macromolecular crowding inside human cultured cells by in-cell NMR spectroscopy are crucial not only for explicit understanding of their cellular functions but also for applications in medical and pharmaceutical sciences. In-cell NMR experiments using human cultured cells however suffer from low sensitivity, thus pseudocontact shifts from protein-tagged paramagnetic lanthanoid ions, analysed...
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09-22-2016 06:26 AM
[NMR paper] A Two-Armed Lanthanoid-Chelating Paramagnetic NMR Probe Linked to Proteins via Thioether Linkages.
A Two-Armed Lanthanoid-Chelating Paramagnetic NMR Probe Linked to Proteins via Thioether Linkages.
Related Articles A Two-Armed Lanthanoid-Chelating Paramagnetic NMR Probe Linked to Proteins via Thioether Linkages.
Chemistry. 2014 Apr 15;
Authors: Liu WM, Skinner SP, Timmer M, Blok A, Hass MA, Filippov DV, Overhand M, Ubbink M
Abstract
Paramagnetic NMR probes provide valuable long-range structural information on proteins and protein complexes. A new, stable, two-armed lanthanoid probe is reported that can be attached to a protein...
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04-17-2014 12:03 PM
The application of paramagnetic lanthanoid ions in NMR spectroscopy on proteins
The application of paramagnetic lanthanoid ions in NMR spectroscopy on proteins
Publication date: Available online 4 November 2013
Source:Coordination Chemistry Reviews</br>
Author(s): Wei-Min Liu , Mark Overhand , Marcellus Ubbink</br>
Lanthanoids are gaining popularity as paramagnetic centers for high resolution nuclear magnetic resonance (NMR) spectroscopy. They provide valuable angular and long-distance restraints for structure calculations of proteins and protein complexes. The introduction of lanthanoids into a protein sample is complicated by the many...
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11-05-2013 06:34 AM
[NMR paper] Detection and characterization of xenon-binding sites in proteins by 129Xe NMR spectr
Detection and characterization of xenon-binding sites in proteins by 129Xe NMR spectroscopy.
Related Articles Detection and characterization of xenon-binding sites in proteins by 129Xe NMR spectroscopy.
J Mol Biol. 2002 Sep 13;322(2):425-40
Authors: Rubin SM, Lee SY, Ruiz EJ, Pines A, Wemmer DE
Xenon-binding sites in proteins have led to a number of applications of xenon in biochemical and structural studies. Here we further develop the utility of 129Xe NMR in characterizing specific xenon-protein interactions. The sensitivity of the 129Xe...
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11-24-2010 08:58 PM
Characterization of caged compounds binding to proteins by NMR spectroscopy.
Characterization of caged compounds binding to proteins by NMR spectroscopy.
Characterization of caged compounds binding to proteins by NMR spectroscopy.
Biochem Biophys Res Commun. 2010 Aug 27;
Authors: Bandorowicz-Pikula J, Buchet R, Cañada FJ, Clémancey M, Groves P, Jiménez-Barbero J, Lancelin JM, Marcillat O, Pikula S, Sekrecka-Belniak A, Strzelecka-Kiliszek A
Photolysable caged ligands are used to investigate protein function and activity. Here, we investigate the binding properties of caged nucleotides and their photo released...
Characterization of lanthanoid-binding proteins using NMR spectroscopy
Linear Mode
