NMR paper Characterization of fast-tumbling isotropic bicelles by PFG diffusion NMR.

		BioNMR > Educational resources > Journal club
[NMR paper] Characterization of fast-tumbling isotropic bicelles by PFG diffusion NMR.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

12-15-2015, 08:09 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Characterization of fast-tumbling isotropic bicelles by PFG diffusion NMR.

Characterization of fast-tumbling isotropic bicelles by PFG diffusion NMR.

Characterization of fast-tumbling isotropic bicelles by PFG diffusion NMR.

Magn Reson Chem. 2015 Dec 14;

Authors: Liebau J, Ye W, Mäler L

Abstract
Small isotropic bicelles are versatile membrane mimetics, which, in contrast to micelles, provide a lipid bilayer and are at the same time suitable for solution-state NMR studies. The lipid composition of the bilayer is flexible allowing for incorporation of various head groups and acyl chain types. In bicelles, lipids are solubilized by detergents, which are localized in the rim of the disk-shaped lipid bilayer. Bicelles have been characterized by a broad array of biophysical methods, pulsed-field gradient NMR (PFG NMR) being one of them. PFG NMR can readily be used to measure diffusion coefficients of macromolecules. It is thus employed to characterize bicelle size and morphology. Even more importantly, PFG NMR can be used to study the degree of protein association to membranes. Here, we present the advances that have been made in producing small, fast-tumbling isotropic bicelles from a variety of lipids and detergents, together with insights on the morphology of such mixtures gained from PFG NMR. Furthermore, we review approaches to study protein-membrane interaction by PFG NMR. Copyright © 2015 John Wiley & Sons, Ltd.

PMID: 26662467 [PubMed - as supplied by publisher]

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Membrane interactions in small fast-tumbling bicelles as studied by (31)P NMR. Membrane interactions in small fast-tumbling bicelles as studied by (31)P NMR. Membrane interactions in small fast-tumbling bicelles as studied by (31)P NMR. Biochim Biophys Acta. 2014 Dec 9; Authors: Bodor A, Kövér KE, Mäler L Abstract Small fast-tumbling bicelles are ideal for studies of membrane interactions at molecular level; they allow analysis of lipid properties using solution-state NMR. In the present study we used (31)P NMR relaxation to obtain detailed information on lipid head-group dynamics. We explored the effect...	nmrlearner	Journal club	0	12-17-2014 09:43 PM
Combined zero-quantum and spin-diffusion mixing for efficient homonuclear correlation spectroscopy under fast MAS: broadband recoupling and detection of long-range correlations Combined zero-quantum and spin-diffusion mixing for efficient homonuclear correlation spectroscopy under fast MAS: broadband recoupling and detection of long-range correlations Abstract Fast magic angle spinning (MAS) NMR spectroscopy is emerging as an essential analytical and structural biology technique. Large resolution and sensitivity enhancements observed under fast MAS conditions enable structural and dynamics analysis of challenging systems, such as large macromolecular assemblies and isotopically dilute samples, using only a fraction of...	nmrlearner	Journal club	0	11-25-2014 07:13 AM
[NMR paper] Local Isotropic Diffusion Approximation for Coupled Internal and Overall Molecular Motions in NMR Spin Relaxation. Local Isotropic Diffusion Approximation for Coupled Internal and Overall Molecular Motions in NMR Spin Relaxation. Related Articles Local Isotropic Diffusion Approximation for Coupled Internal and Overall Molecular Motions in NMR Spin Relaxation. J Phys Chem B. 2014 Aug 28; Authors: Gill ML, Palmer AG Abstract The present work demonstrates that NMR spin relaxation rate constants for molecules interconverting between states with different diffusion tensors can be modeled theoretically by combining orientational correlation...	nmrlearner	Journal club	0	08-29-2014 05:36 PM
Characterization of Mapcho Bicelles - Model Membranes for the NMR Study of Membrane Proteins and Peptides Characterization of Mapcho Bicelles - Model Membranes for the NMR Study of Membrane Proteins and Peptides Publication date: 28 January 2014 Source:Biophysical Journal, Volume 106, Issue 2, Supplement 1</br> Author(s): Maïwenn Beaugrand , Alexandre A. Arnold , Philip T.F. Williamson , Isabelle Marcotte</br> </br></br> </br></br> More...	nmrlearner	Journal club	0	01-29-2014 12:50 AM
Isotropic Bicelles Stabilize the Juxtamembrane Regionof the Influenza M2 Protein for Solution NMR Studies Isotropic Bicelles Stabilize the Juxtamembrane Regionof the Influenza M2 Protein for Solution NMR Studies http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/bi401035m/aop/images/medium/bi-2013-01035m_0009.gif Biochemistry DOI: 10.1021/bi401035m http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/vg4boQJ0_fk More...	nmrlearner	Journal club	0	11-15-2013 12:34 AM
[NMR paper] Isotropic bicelles stabilize the juxtamembrane region of the influenza M2 protein for solution NMR studies. Isotropic bicelles stabilize the juxtamembrane region of the influenza M2 protein for solution NMR studies. Related Articles Isotropic bicelles stabilize the juxtamembrane region of the influenza M2 protein for solution NMR studies. Biochemistry. 2013 Oct 29; Authors: Claridge JK, Aittoniemi J, Cooper DM, Schnell JR Abstract The protein M2 from influenza is a tetrameric membrane protein with several roles in the viral life cycle. The transmembrane helix (TMH) of M2 has proton channel activity that is required for unpackaging the viral...	nmrlearner	Journal club	0	10-31-2013 02:18 PM
Fast NMR Data Acquisition From Bicelles Containing a Membrane-Associated Peptide at Natural-Abundance. Fast NMR Data Acquisition From Bicelles Containing a Membrane-Associated Peptide at Natural-Abundance. Fast NMR Data Acquisition From Bicelles Containing a Membrane-Associated Peptide at Natural-Abundance. J Phys Chem B. 2011 Sep 22; Authors: Yamamoto K, Vivekanandan S, Ramamoorthy A Abstract In spite of recent technological advances in NMR spectroscopy, its low sensitivity continues to be a major limitation particularly for the structural studies of membrane proteins. The need for a large quantity of a membrane protein and acquisition of...	nmrlearner	Journal club	0	09-24-2011 04:11 PM
Li Ion Diffusion in the Anode Material Li12Si7: Ultrafast Quasi-1D Diffusion and Two Distinct Fast 3D Jump Processes Separately Revealed by 7Li NMR Relaxometry Li Ion Diffusion in the Anode Material Li12Si7: Ultrafast Quasi-1D Diffusion and Two Distinct Fast 3D Jump Processes Separately Revealed by 7Li NMR Relaxometry Alexander Kuhn, Puravankara Sreeraj, Rainer Po?ttgen, Hans-Dieter Wiemho?fer, Martin Wilkening and Paul Heitjans http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja2020108/aop/images/medium/ja-2011-020108_0005.gif Journal of the American Chemical Society DOI: 10.1021/ja2020108 http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA...	nmrlearner	Journal club	0	06-28-2011 04:32 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off