Inorganic pyrophosphatase catalyzes the conversion of pyrophosphate to phosphate and is often critical for driving reactions forward in cellular processes such as nucleic acid and protein synthesis. Commonly used methods for quantifying pyrophosphatase enzyme activity employ reacting liberated phosphate with a second molecule to produce absorbance changes or employing a second enzyme in coupled reactions to produce a product with a detectable absorbance. In this investigation, a novel...
[NMR paper] An NMR-based Assay for the ex vivo Determination of Soluble CD73 Activity in Serum.
An NMR-based Assay for the ex vivo Determination of Soluble CD73 Activity in Serum.
Related Articles An NMR-based Assay for the ex vivo Determination of Soluble CD73 Activity in Serum.
Anal Chem. 2020 Sep 28;:
Authors: He Y, Zhao Y, Nelson DM, Klippel A, Reily MD
Abstract
Extracellular adenosine, produced through the activity of the ecto-5'-nucleotidase CD73, elicits potent immune-suppressive effects and its upregulation in tumor cells as well as in stromal and immune cell subsets within the tumor microenvironment is hypothesized...
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[NMR paper] Characterization of LP-Z Lipoprotein Particles and Quantification in Subjects with Liver Disease Using a Newly Developed NMR-Based Assay.
Characterization of LP-Z Lipoprotein Particles and Quantification in Subjects with Liver Disease Using a Newly Developed NMR-Based Assay.
Related Articles Characterization of LP-Z Lipoprotein Particles and Quantification in Subjects with Liver Disease Using a Newly Developed NMR-Based Assay.
J Clin Med. 2020 Sep 10;9(9):
Authors: Bedi S, Garcia E, Jeyarajah EJ, Shalaurova I, Perez-Matos MC, Jiang ZG, Dullaart RPF, Matyus SP, Kirk WJ, Otvos JD, Davidson WS, Connelly MA
Abstract
BACKGROUND: Lipoprotein particles with abnormal...
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09-17-2020 07:46 AM
[NMR paper] HOPPI-NMR: Hot-Peptide-Based Screening Assay for Inhibitors of Protein-Protein Interactions by NMR.
HOPPI-NMR: Hot-Peptide-Based Screening Assay for Inhibitors of Protein-Protein Interactions by NMR.
HOPPI-NMR: Hot-Peptide-Based Screening Assay for Inhibitors of Protein-Protein Interactions by NMR.
ACS Med Chem Lett. 2020 May 14;11(5):1047-1053
Authors: Brancaccio D, Di Maro S, Cerofolini L, Giuntini S, Fragai M, Luchinat C, Tomassi S, Limatola A, Russomanno P, Merlino F, Novellino E, Carotenuto A
Abstract
Protein-protein interactions (PPIs) contribute to the onset and/or progression of several diseases, especially cancer, and...
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05-22-2020 03:58 PM
[NMR paper] Fluorine NMR-based assay in living mammalian cells.
Fluorine NMR-based assay in living mammalian cells.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Fluorine NMR-based assay in living mammalian cells.
Anal Biochem. 2015 Dec 11;
Authors: Veronesi M, Giacomina F, Romeo E, Castellani B, Ottonello G, Lambruschini C, Garau G, Scarpelli R, Bandiera T, Piomelli D, Dalvit C
Abstract
Nuclear Magnetic Resonance (NMR)-based screening has been recognized as a powerful approach for the identification...
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[NMR paper] Applications of NMR-based PRE and EPR-based DEER spectroscopy to homodimer chain exchange characterization and structure determination.
Applications of NMR-based PRE and EPR-based DEER spectroscopy to homodimer chain exchange characterization and structure determination.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles Applications of NMR-based PRE and EPR-based DEER spectroscopy to homodimer chain exchange characterization and structure determination.
Methods Mol Biol. 2014;1091:215-27
Authors: Yang Y, Ramelot TA, Ni S, McCarrick RM, Kennedy MA
Abstract
Cracking the molecular weight barrier: Fragment screening of an aminotransferase using an NMR-based functional assay.
Cracking the molecular weight barrier: Fragment screening of an aminotransferase using an NMR-based functional assay.
Cracking the molecular weight barrier: Fragment screening of an aminotransferase using an NMR-based functional assay.
Bioorg Med Chem Lett. 2011 Jul 21;
Authors: Mendoza R, Petros AM, Liu Y, Thimmapaya R, Surowy CS, Leise WF, Pereda-Lopez A, Panchal SC, Sun C
NMR-based screening of protein targets has become a well established part of the drug discovery process especially with respect to fragments. However, as target size increases...
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[NMR paper] NMR structure of an archaeal homologue of ribonuclease P protein Rpp29.
NMR structure of an archaeal homologue of ribonuclease P protein Rpp29.
Related Articles NMR structure of an archaeal homologue of ribonuclease P protein Rpp29.
Biochemistry. 2003 Nov 25;42(46):13541-50
Authors: Sidote DJ, Hoffman DW
A protein component of the Archaeoglobus fulgidus RNase P was expressed in Escherichia coli, purified, and structurally characterized using multidimensional NMR methods. The dominant structural feature of this 11 kDa protein is a sheet of six antiparallel beta-strands, wrapped around a core of conserved...
![Characterization of an archaeal inorganic pyrophosphatase from Sulfolobus islandicus using a [(31)P]-NMR-based assay](http://www.bionmr.com/forum/iconimages/journal-club-9/characterization-archaeal-inorganic-pyrophosphatase-sulfolobus-islandicus-using-%5B-31-p%5D-nmr-based-assay_ltr.gif)
Characterization of an archaeal inorganic pyrophosphatase from Sulfolobus islandicus using a [(31)P]-NMR-based assay
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