Related ArticlesCephaloridine-induced nephrotoxicity in the Fischer 344 rat: proton NMR spectroscopic studies of urine and plasma in relation to conventional clinical chemical and histopathological assessments of nephronal damage.
Arch Toxicol. 1992;66(8):525-37
Authors: Anthony ML, Gartland KP, Beddell CR, Lindon JC, Nicholson JK
The acute toxicological effects of the nephrotoxic antibiotic cephaloridine (CPH, 0-1500 mg/kg) in male Fischer 344 (F344) rats, have been investigated over 48 h using clinical chemistry, histopathology and proton nuclear magnetic resonance (1H NMR) spectroscopy of urine and plasma. High field (400 and 600 MHz)1H NMR urinalysis revealed increased excretion of lactic acid, acetoacetate, alanine, valine, lysine, glutamine and glutamate and a severe, time-dependent glycosuria. A major change observed in urine of CPH-treated animals was the dose-dependent increase in HB which may relate to altered energy metabolism. CPH also caused dose-dependent decreases in the urinary excretion of hippurate, allantoin and protein (conventional assay). This abnormal metabolic profile is consistent with a functional defect in the S1/S2 regions of the proximal tubule, and was confirmed by histology post mortem. Functional changes observed included elevations in blood urea nitrogen (BUN) and urine flow rate (UFR) and dose-related decreases in urine osmolality. Spin-echo 1H NMR spectroscopic analysis of lyophilised plasma, reconstituted with 2H2O revealed an abnormal phase modulation of the methyl signal from free alanine and it is postulated that this is due to the release of transaminases from damaged tissue which via a reversible conversion to pyruvate, cause variable deuteration of alanine at the alpha-CH position. This observation suggests that 1H NMR spectral patterns are also dependent on the level of plasma transaminases and this may provide a novel indicator of tissue damage.
NMR Detection of pH-Dependent Histidine–Water Proton Exchange Reveals the Conduction Mechanism of a Transmembrane Proton Channel
NMR Detection of pH-Dependent Histidine–Water Proton Exchange Reveals the Conduction Mechanism of a Transmembrane Proton Channel
Fanghao Hu, Klaus Schmidt-Rohr and Mei Hong
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja2081185/aop/images/medium/ja-2011-081185_0008.gif
Journal of the American Chemical Society
DOI: 10.1021/ja2081185
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/C3pPoB5_PR8
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[NMR paper] Proton NMR spectroscopy shows lipids accumulate in skeletal muscle in response to burn trauma-induced apoptosis.
Proton NMR spectroscopy shows lipids accumulate in skeletal muscle in response to burn trauma-induced apoptosis.
Related Articles Proton NMR spectroscopy shows lipids accumulate in skeletal muscle in response to burn trauma-induced apoptosis.
FASEB J. 2005 Sep;19(11):1431-40
Authors: Astrakas LG, Goljer I, Yasuhara S, Padfield KE, Zhang Q, Gopalan S, Mindrinos MN, Dai G, Yu YM, Martyn JA, Tompkins RG, Rahme LG, Tzika AA
Burn trauma triggers hypermetabolism and muscle wasting via increased cellular protein degradation and apoptosis. Proton...
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[NMR paper] Direct refinement against proton-proton dipolar couplings in NMR structure determinat
Direct refinement against proton-proton dipolar couplings in NMR structure determination of macromolecules.
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J Magn Reson. 2000 Feb;142(2):393-6
Authors: Tjandra N, Marquardt J, Clore GM
The computational tools necessary for making use of (1)H-(1)H dipolar couplings in macromolecular structure refinement are presented. Potentials are described for direct refinement against (1)H-(1)H dipolar couplings of known sign as well...
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[NMR paper] In vitro proton and phosphorus NMR spectroscopic analysis of murine (C57Bl/6J) brain
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NMR Biomed. 1999 Nov;12(7):463-70
Authors: Yao FS, Caserta MT, Wyrwicz AM
We report for the first time in vitro proton and phosphorus NMR spectroscopic analyses of murine brain development from fetal to adult stages. Chloroform-methanol extracts from C57B16/J mouse brain, at ages ranging from 15 days in utero (F15) to adult, permitted...
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Mechanisms of proton conduction and gating in influenza m2 proton channels from solid
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Science. 2010 Oct 22;330(6003):505-8
Authors: Hu F, Luo W, Hong M
The M2 protein of influenza viruses forms an acid-activated tetrameric proton channel. We used solid-state nuclear magnetic resonance spectroscopy to determine the structure and functional dynamics of the pH-sensing and proton-selective histidine-37 in M2 bound to a...
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[NMR paper] NMR spectroscopic analysis of the DNA conformation induced by the human testis determ
NMR spectroscopic analysis of the DNA conformation induced by the human testis determining factor SRY.
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Biochemistry. 1995 Sep 19;34(37):11998-2004
Authors: Werner MH, Bianchi ME, Gronenborn AM, Clore GM
The conformation of an eight base pair DNA oligonucleotide duplex bound to the human testis determining factor SRY and the orientation of the protein domain within the complex have been analyzed by a variety of NMR methods...
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[NMR paper] 1H-NMR spectroscopy as a means of monitoring nephrotoxicity as exemplified by studies
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Related Articles 1H-NMR spectroscopy as a means of monitoring nephrotoxicity as exemplified by studies with cephaloridine.
Hum Exp Toxicol. 1992 Jan;11(1):35-41
Authors: Murgatroyd LB, Pickford RJ, Smith IK, Wilson ID, Middleton BJ
1. Male albino rats were dosed intravenously with either 0.9% saline or cephaloridine in saline at doses of 650, 750 or 950 mg kg-1 d-1 for 7 d. 2. Urine analysis on day 3, after two doses of cephaloridine...
Cephaloridine-induced nephrotoxicity in the Fischer 344 rat: proton NMR spectroscopic
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