Cellular Assays for Ferredoxins: A Strategy for UnderstandingElectron Flow through Protein Carriers That Link Metabolic Pathways

		BioNMR > Educational resources > Journal club
Cellular Assays for Ferredoxins: A Strategy for UnderstandingElectron Flow through Protein Carriers That Link Metabolic Pathways

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

12-15-2016, 02:04 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Cellular Assays for Ferredoxins: A Strategy for UnderstandingElectron Flow through Protein Carriers That Link Metabolic Pathways

Cellular Assays for Ferredoxins: A Strategy for UnderstandingElectron Flow through Protein Carriers That Link Metabolic Pathways

Biochemistry
DOI: 10.1021/acs.biochem.6b00831

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Optimization of protein samples for NMR using thermal shift assays Optimization of protein samples for NMR using thermal shift assays Abstract Maintaining a stable fold for recombinant proteins is challenging, especially when working with highly purified and concentrated samples at temperatures >20Â*Â°C. Therefore, it is worthwhile to screen for different buffer components that can stabilize protein samples. Thermal shift assays or ThermoFluorÂ® provide a high-throughput screening method to assess the thermal stability of a sample under several conditions simultaneously. Here, we describe a thermal shift assay that...	nmrlearner	Journal club	0	03-22-2016 01:46 PM
[NMR paper] Optimization of protein samples for NMR using thermal shift assays. Optimization of protein samples for NMR using thermal shift assays. Optimization of protein samples for NMR using thermal shift assays. J Biomol NMR. 2016 Mar 17; Authors: Kozak S, Lercher L, Karanth MN, Meijers R, Carlomagno T, Boivin S Abstract Maintaining a stable fold for recombinant proteins is challenging, especially when working with highly purified and concentrated samples at temperatures >20*°C. Therefore, it is worthwhile to screen for different buffer components that can stabilize protein samples. Thermal shift assays...	nmrlearner	Journal club	0	03-18-2016 05:23 PM
Increasing Throughput in Protein Thermal Shift Assays - Genetic Engineering & Biotechnology News <img alt="" height="1" width="1"> Increasing Throughput in Protein Thermal Shift Assays Genetic Engineering & Biotechnology News Historically, protein interaction studies have been measured using an array of techniques, including nuclear magnetic resonance, differential scanning calorimetry, and surface plasma resonance imaging. Additionally, protein melt screening methods used ... Increasing Throughput in Protein Thermal Shift Assays - Genetic Engineering & Biotechnology News More...	nmrlearner	Online News	0	04-11-2015 12:04 AM
Sex Hormone Levels at Midlife Linked to Bad Cholesterol Carriers that Increase ... - Gant Daily http://www.bionmr.com//t3.gstatic.com/images?q=tbn:ANd9GcT4L3iPAkpxQbTj4nCSM4gd0T-SsIjdltF8wisSryyBT76uDgtBgwoX9lP9_wtERWVfXJHm-T8 Medical Daily <img alt="" height="1" width="1" /> Sex Hormone Levels at Midlife Linked to Bad Cholesterol Carriers that Increase ... Gant Daily El Khoudary and her colleagues used nuclear magnetic resonance spectroscopy to measure the size, distribution and concentration of lipoproteins that carry cholesterol in the blood. The Pitt Public Health team found that as estrogen levels fall, women ... Sex Hormones During Menopause Determine If Bad Cholesterol Will...	nmrlearner	Online News	0	06-30-2014 11:39 PM
[NMR paper] Metabolic changes during cellular senescence investigated by proton NMR-spectroscopy. Metabolic changes during cellular senescence investigated by proton NMR-spectroscopy. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Metabolic changes during cellular senescence investigated by proton NMR-spectroscopy. Mech Ageing Dev. 2013 Mar;134(3-4):130-8 Authors: Gey C, Seeger K Abstract Cellular senescence is of growing interest due to its role in tumour suppression and its contribution to organismic ageing. This cellular state can be...	nmrlearner	Journal club	0	10-01-2013 11:15 PM
Elucidating metabolic pathways for amino acid incorporation into dragline spider silk using 13C enrichment and solid state NMR. Elucidating metabolic pathways for amino acid incorporation into dragline spider silk using 13C enrichment and solid state NMR. Elucidating metabolic pathways for amino acid incorporation into dragline spider silk using 13C enrichment and solid state NMR. Comp Biochem Physiol A Mol Integr Physiol. 2011 Jul;159(3):219-24 Authors: Creager MS, Izdebski T, Brooks AE, Lewis RV Abstract Spider silk has been evolutionarily optimized for contextual mechanical performance over the last 400 Ma. Despite precisely balanced mechanical properties,...	nmrlearner	Journal club	0	09-02-2011 05:40 PM
[NMR paper] NMR and protein folding: equilibrium and stopped-flow studies. NMR and protein folding: equilibrium and stopped-flow studies. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles NMR and protein folding: equilibrium and stopped-flow studies. Protein Sci. 1993 Dec;2(12):2007-14 Authors: Frieden C, Hoeltzli SD, Ropson IJ NMR studies are now unraveling the structure of intermediates...	nmrlearner	Journal club	0	08-22-2010 03:01 AM
[NMR tweet] Is it Possible to Extract Brain Metabolic Pathways Information from In Vivo H Nuclear Is it Possible to Extract Brain Metabolic Pathways Information from In Vivo H Nuclear Magnetic Resonance Spectroscopy ? http://bit.ly/9fUMgB Published by PhysicsPaper (New Physics Papers) on 2010-08-17T02:45:59Z Source: Twitter	nmrlearner	Twitter NMR	0	08-16-2010 11:59 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off