In-cell structural biology aims at extracting structural information about proteins or nucleic acids in their native, cellular environment. This emerging field holds great promise and is already providing new facts and outlooks of interest at both fundamental and applied levels. NMR spectroscopy has important contributions on this stage: It brings information on a broad variety of nuclei at the atomic scale, which ensures its great versatility and uniqueness. Here, we detail the methods, the...
Magic-angle-spinning NMR used to probe protein/microtubule assembly at atomic scale - UD Daily
Magic-angle-spinning NMR used to probe protein/microtubule assembly at atomic scale - UD Daily
Magic-angle-spinning NMR used to probe protein/microtubule assembly at atomic scale UD Daily5:30 p.m., Nov. 24, 2015--A latticework of tiny tubes called microtubules gives your cells their shape and also acts like a railroad track that essential proteins ...
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Magic-angle-spinning NMR used to probe protein/microtubule assembly at atomic scale - Phys.Org
Magic-angle-spinning NMR used to probe protein/microtubule assembly at atomic scale - Phys.Org
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Magic-angle-spinning NMR used to probe protein/microtubule assembly at atomic scale
Phys.Org
"Just as we are always moving our arms and legs about, proteins are very dynamic. They do not stand still," Polenova says. "These motions are essential to their biological function, and NMR spectroscopy is the only technique...
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12-02-2017 02:54 PM
[NMR paper] Beyond Structural Biology to Functional Biology: Solid-State NMR Experiments and Strategies for Understanding the M2 Proton Channel Conductance.
Beyond Structural Biology to Functional Biology: Solid-State NMR Experiments and Strategies for Understanding the M2 Proton Channel Conductance.
Related Articles Beyond Structural Biology to Functional Biology: Solid-State NMR Experiments and Strategies for Understanding the M2 Proton Channel Conductance.
J Phys Chem B. 2017 Apr 20;:
Authors: Qin H, Miao Y, Cross TA, Fu R
Abstract
In terms of structural biology, solid-state NMR experiments and strategies have been well established for resonance assignments leading to the...
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04-21-2017 03:35 PM
Magic-angle-spinning NMR used to probe protein/microtubule assembly at atomic scale - UD Daily
Magic-angle-spinning NMR used to probe protein/microtubule assembly at atomic scale - UD Daily
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UD Daily
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Magic-angle-spinning NMR used to probe protein/microtubule assembly at atomic scale
UD Daily
5:30 p.m., Nov. 24, 2015--A latticework of tiny tubes called microtubules gives your cells their shape and also acts like a railroad track that essential proteins travel on. But if there is a glitch in the connection between...
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07-18-2016 03:40 AM
[NMR paper] Micro-scale NMR Experiments for Monitoring the Optimization of Membrane Protein Solutions for Structural Biology.
Micro-scale NMR Experiments for Monitoring the Optimization of Membrane Protein Solutions for Structural Biology.
Micro-scale NMR Experiments for Monitoring the Optimization of Membrane Protein Solutions for Structural Biology.
Bio Protoc. 2015 Jul 20;5(14)
Authors: Horst R, Wüthrich K
Abstract
Reconstitution of integral membrane proteins (IMP) in aqueous solutions of detergent micelles has been extensively used in structural biology, using either X-ray crystallography or NMR in solution. Further progress could be achieved by...
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04-15-2016 05:06 PM
[NMR paper] A Unique Tool for Cellular Structural Biology: In-cell NMR.
A Unique Tool for Cellular Structural Biology: In-cell NMR.
Related Articles A Unique Tool for Cellular Structural Biology: In-cell NMR.
J Biol Chem. 2015 Dec 16;
Authors: Luchinat E, Banci L
Abstract
Conventional structural and chemical biology approaches are applied to macromolecules extrapolated from their native context. When doing so, important structural and functional features of macromolecules may be lost, which depend on their native network of interactions within the cell. In-cell nuclear magnetic resonance is a branch...
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12-28-2015 12:26 AM
Collagen atomic scale molecular disorder in ochronotic cartilage from an alkaptonuria patient, observed by solid state NMR.
Collagen atomic scale molecular disorder in ochronotic cartilage from an alkaptonuria patient, observed by solid state NMR.
Collagen atomic scale molecular disorder in ochronotic cartilage from an alkaptonuria patient, observed by solid state NMR.
J Inherit Metab Dis. 2011 Jul 7;
Authors: Chow WY, Taylor AM, Reid DG, Gallagher JA, Duer MJ
In pilot studies of the usefulness of solid state nuclear magnetic resonance spectroscopy in characterizing chemical and molecular structural effects of alkaptonuria on connective tissue, we have obtained...
In-Cell Structural Biology by NMR: The Benefits of the Atomic Scale
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