Related ArticlesIn-Cell Solid-State NMR: An Emerging Technique for the Study of Biological Membranes.
Biophys J. 2015 Dec 15;109(12):2461-6
Authors: Warnet XL, Arnold AA, Marcotte I, Warschawski DE
Abstract
Biological molecular processes are often studied in model systems, which simplifies their inherent complexity but may cause investigators to lose sight of the effects of the molecular environment. Information obtained in this way must therefore be validated by experiments in the cell. NMR has been used to study biological cells since the early days of its development. The first NMR structural studies of a protein inside a cell (by solution-state NMR) and of a membrane protein (by solid-state NMR) were published in 2001 and 2011, respectively. More recently, dynamic nuclear polarization, which has been used to enhance the signal in solid-state NMR, has also been applied to the study of frozen cells. Much progress has been made in the past 5*years, and in this review we take stock of this new technique, which is particularly appropriate for the study of biological membranes.
[NMR paper] Solid-state NMR: An emerging technique in structural biology of self-assemblies.
Solid-state NMR: An emerging technique in structural biology of self-assemblies.
Related Articles Solid-state NMR: An emerging technique in structural biology of self-assemblies.
Biophys Chem. 2015 Jul 16;
Authors: Habenstein B, Loquet A
Abstract
Protein self-assemblies are ubiquitous biological systems involved in many cellular processes, ranging from bacterial and viral infection to the propagation of neurodegenerative disorders. Studying the atomic three-dimensional structures of protein self-assemblies is a particularly...
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[NMR paper] A 2H solid-state NMR study of lipid clustering by cationic antimicrobial and cell-penetrating peptides in model bacterial membranes.
A 2H solid-state NMR study of lipid clustering by cationic antimicrobial and cell-penetrating peptides in model bacterial membranes.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-cellhub.gif Related Articles A 2H solid-state NMR study of lipid clustering by cationic antimicrobial and cell-penetrating peptides in model bacterial membranes.
Biophys J. 2013 Nov 19;105(10):2333-42
Authors: Kwon B, Waring AJ, Hong M
Abstract
Domain formation in bacteria-mimetic membranes...
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[NMR paper] In-cell NMR: an emerging approach for monitoring metal-related events in living cells.
In-cell NMR: an emerging approach for monitoring metal-related events in living cells.
Related Articles In-cell NMR: an emerging approach for monitoring metal-related events in living cells.
Metallomics. 2013 Nov 8;
Authors: Li H, Sun H
Abstract
In-cell NMR, an isotope-assisted multi-dimensional NMR technique, has been proven to be successful in the investigation of protein dynamics, folding, conformational changes induced by binding events, posttranslational modification in the complex native environments, as well as in vivo drug...
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[NMR paper] Impact of oxidized phospholipids on the structural and dynamic organization of phospholipid membranes: a combined DSC and solid state NMR study.
Impact of oxidized phospholipids on the structural and dynamic organization of phospholipid membranes: a combined DSC and solid state NMR study.
Impact of oxidized phospholipids on the structural and dynamic organization of phospholipid membranes: a combined DSC and solid state NMR study.
Faraday Discuss. 2013;161:499-513; discussion 563-89
Authors: Wallgren M, Beranova L, Pham QD, Linh K, Lidman M, Procek J, Cyprych K, Kinnunen PK, Hof M, Gröbner G
Abstract
Membranes undergo severe changes under oxidative stress conditions due to the...
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06-29-2013 11:49 AM
[NMR paper] Interactions of lipopolysaccharide with lipid membranes, raft models - a solid state NMR study.
Interactions of lipopolysaccharide with lipid membranes, raft models - a solid state NMR study.
Related Articles Interactions of lipopolysaccharide with lipid membranes, raft models - a solid state NMR study.
Biochim Biophys Acta. 2013 Apr 5;
Authors: Ciesielski F, Griffin DC, Rittig M, Moriyón I, Bonev BB
Abstract
Lipopolysaccharide (LPS) is a major component of the external leaflet of bacterial outer membranes, key pro-inflammatory factor and an important mediator of host-pathogen interactions. In host cells it activates the complement...
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04-10-2013 07:21 PM
Solid-State (19)F-NMR of Peptides in Native Membranes.
Solid-State (19)F-NMR of Peptides in Native Membranes.
Solid-State (19)F-NMR of Peptides in Native Membranes.
Top Curr Chem. 2011 May 20;
Authors: Koch K, Afonin S, Ieronimo M, Berditsch M, Ulrich AS
To understand how membrane-active peptides (MAPs) function in vivo, it is essential to obtain structural information about them in their membrane-bound state. Most biophysical approaches rely on the use of bilayers prepared from synthetic phospholipids, i.e. artificial model membranes. A particularly successful structural method is solid-state NMR,...
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05-21-2011 07:51 PM
[NMR paper] Exploring the calcium-binding site in photosystem II membranes by solid-state (113)Cd
Exploring the calcium-binding site in photosystem II membranes by solid-state (113)Cd NMR.
Related Articles Exploring the calcium-binding site in photosystem II membranes by solid-state (113)Cd NMR.
Biochemistry. 2000 Jun 13;39(23):6751-5
Authors: Matysik J, Alia A, Nachtegaal G, van Gorkom HJ, Hoff AJ, de Groot HJ
Calcium (Ca(2+)) is an essential cofactor for photosynthetic oxygen evolution. Although the involvement of Ca(2+) at the oxidizing side of photosystem II of plants has been known for a long time, its ligand interactions and mode of...
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[NMR paper] Interaction of a type II myosin with biological membranes studied by 2H solid state N
Interaction of a type II myosin with biological membranes studied by 2H solid state NMR.
Related Articles Interaction of a type II myosin with biological membranes studied by 2H solid state NMR.
Biochemistry. 1998 Apr 21;37(16):5582-8
Authors: Arêas JA, Gröbner G, Glaubitz C, Watts A
Deuterium nuclear magnetic resonance spectroscopy (2H NMR) has been employed to investigate the interaction of lung type II myosin protein with neutral bilayers containing dimyristoylphosphatidylcholine (DMPC) as the only constituent and mixed bilayers containing...
In-Cell Solid-State NMR: An Emerging Technique for the Study of Biological Membranes.
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