NMR has the resolution and specificity to determine atomic-level protein structures of isotopically labeled proteins in complex environments, and with the sensitivity gains conferred by dynamic nuclear polarization (DNP), NMR has the sensitivity to detect proteins at their endogenous concentrations. However, DNP sensitivity enhancements are critically dependent on experimental conditions and sample composition. While some of these conditions are theoretically compatible with cellular viability,...
[ASAP] In-Cell Sensitivity-Enhanced NMR of Intact Viable Mammalian Cells
In-Cell Sensitivity-Enhanced NMR of Intact Viable Mammalian Cells
Rupam Ghosh, Yiling Xiao, Jaka Kragelj, and Kendra K. Frederick
https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.1c06680/20211101/images/medium/ja1c06680_0007.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.1c06680
http://feeds.feedburner.com/~r/acs/jacsat/~4/Px8tejOwLAA
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11-02-2021 07:28 AM
[NMR paper] In-Cell NMR of Intrinsically Disordered Proteins in Mammalian Cells.
In-Cell NMR of Intrinsically Disordered Proteins in Mammalian Cells.
Related Articles In-Cell NMR of Intrinsically Disordered Proteins in Mammalian Cells.
Methods Mol Biol. 2020;2141:873-893
Authors: Gerez JA, Prymaczok NC, Riek R
Abstract
In-cell NMR enables structural insights at atomic resolution of proteins in their natural environment. To date, very few methods have been developed to study proteins by in-cell NMR in mammalian systems. Here we describe a detailed protocol to conduct in-cell NMR on the intrinsically...
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07-23-2020 11:23 PM
[NMR paper] Recording In-Cell NMR-Spectra in Living Mammalian Cells.
Recording In-Cell NMR-Spectra in Living Mammalian Cells.
Related Articles Recording In-Cell NMR-Spectra in Living Mammalian Cells.
Methods Mol Biol. 2020;2141:857-871
Authors: Mate?ko-Burmann I, Burmann BM
Abstract
At the foundation of many cellular processes as well as a large number of diseases is the (mis)folding of important intrinsically disordered proteins (IDPs). Despite tremendous scientific efforts, the factors driving their structural changes within the cellular context remain poorly understood. In-cell NMR...
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07-23-2020 11:23 PM
Dynamic Nuclear Polarization with Electron Decoupling in Intact Human Cells and Cell Lysates #DNPNMR
From The DNP-NMR Blog:
Dynamic Nuclear Polarization with Electron Decoupling in Intact Human Cells and Cell Lysates #DNPNMR
Judge, Patrick T., Erika L. Sesti, Lauren E. Price, Brice J. Albert, Nicholas Alaniva, Edward P. Saliba, Thomas Halbritter, Snorri Th. Sigurdsson, George B. Kyei, and Alexander B. Barnes. “Dynamic Nuclear Polarization with Electron Decoupling in Intact Human Cells and Cell Lysates.” The Journal of Physical Chemistry B, February 21, 2020.
https://doi.org/10.1021/acs.jpcb.9b10494.
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06-13-2020 05:51 PM
[NMR paper] Spectral Comparisons of Mammalian Cells and Intact Organelles by Solid-State NMR.
Spectral Comparisons of Mammalian Cells and Intact Organelles by Solid-State NMR.
Related Articles Spectral Comparisons of Mammalian Cells and Intact Organelles by Solid-State NMR.
J Struct Biol. 2018 May 30;:
Authors: Werby SH, Cegelski L
Abstract
Whole-cell protein profiling, spatial localization, and quantification of activities such as gene transcription and protein translation are possible with modern biochemical and biophysical techniques. Yet, addressing questions of overall compositional changes within a cell - capturing...
[NMR paper] Characterization of proteins by in-cell NMR spectroscopy in cultured mammalian cells.
Characterization of proteins by in-cell NMR spectroscopy in cultured mammalian cells.
Characterization of proteins by in-cell NMR spectroscopy in cultured mammalian cells.
Nat Protoc. 2016 Jun;11(6):1101-1111
Authors: Barbieri L, Luchinat E, Banci L
Abstract
In-Cell Sensitivity-Enhanced NMR of Intact Viable Mammalian Cells
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