[NMR paper] Probing Cell-Surface Interactions in Fungal Cell Walls by High-Resolution 1H-detected Solid-State NMR Spectroscopy
Probing Cell-Surface Interactions in Fungal Cell Walls by High-Resolution 1H-detected Solid-State NMR Spectroscopy
Solid-state NMR (ssNMR) spectroscopy facilitates the non-destructive characterization of structurally heterogeneous biomolecules in their native setting, e.g., comprising proteins, lipids and polysaccharides. Here we demonstrate the utility of high and ultra-high field 1H-detected fast MAS ssNMR spectroscopy, which exhibits increased sensitivity and spectral resolution to further elucidate the atomic-level composition and structural arrangement of the cell wall of...
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10-02-2022 07:47 AM
[NMR paper] Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor
Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor
In-cell NMR is a unique approach to observe the structural and dynamic properties of biological macromolecules at atomic resolution directly in living cells. Protein folding, chemical modifications, and conformational changes induced by ligand binding can be observed. Therefore, this method has great potential in the context of drug development. However, the short lifetime of human cells confined in the NMR spectrometer limits the application range of in-cell...
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03-30-2021 08:29 AM
[NMR paper] 15N isotopic labelling for in-cell protein studies by NMR spectroscopy and single-cell IR synchrotron radiation FTIR microscopy: a correlative study.
15N isotopic labelling for in-cell protein studies by NMR spectroscopy and single-cell IR synchrotron radiation FTIR microscopy: a correlative study.
15N isotopic labelling for in-cell protein studies by NMR spectroscopy and single-cell IR synchrotron radiation FTIR microscopy: a correlative study.
Analyst. 2018 Feb 06;:
Authors: Mitri E, Barbieri L, Vaccari L, Luchinat E
Abstract
The ultimate goal of modern structural biology is to probe protein structures and dynamics in their physiological microenvironment. In-cell NMR...
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02-07-2018 03:41 PM
MIT researchers gain new insight on how flu spreads from cell to cell in the body - The Boston Globe
http://www.bionmr.com//t1.gstatic.com/images?q=tbn:ANd9GcT3p5CIt93IQf_uUwbrb3sh9ojlfvedHGRL5Ay3c-AyIjbJakEnyAAMVEaltCP7tI5mSTrJ9oG-
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MIT researchers gain new insight on how flu spreads from cell to cell in the body
The Boston Globe
Cholesterol molecules (green) binding to M2 influenza protein molecules (grey and purple). The shaded grey shapes represent what the configuration looks like on the surface. By Alyssa Meyers Globe Correspondent November 20, 2017. MIT researchers say ...
Cholesterol helps flu virus escape through host cell's...
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11-22-2017 02:01 PM
[NMR paper] Glycosaminoglycan Binding and Non-Endocytic Membrane Translocation of Cell-Permeable Octaarginine Monitored by Real-Time In-Cell NMR Spectroscopy.
Glycosaminoglycan Binding and Non-Endocytic Membrane Translocation of Cell-Permeable Octaarginine Monitored by Real-Time In-Cell NMR Spectroscopy.
Related Articles Glycosaminoglycan Binding and Non-Endocytic Membrane Translocation of Cell-Permeable Octaarginine Monitored by Real-Time In-Cell NMR Spectroscopy.
Pharmaceuticals (Basel). 2017 Apr 15;10(2):
Authors: Takechi-Haraya Y, Aki K, Tohyama Y, Harano Y, Kawakami T, Saito H, Okamura E
Abstract
Glycosaminoglycans (GAGs), which are covalently-linked membrane proteins at the cell...
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04-20-2017 06:14 PM
[NMR paper] Bacterial cell wall composition and the influence of antibiotics by cell-wall and whole-cell NMR.
Bacterial cell wall composition and the influence of antibiotics by cell-wall and whole-cell NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--highwire.stanford.edu-icons-externalservices-pubmed-roysocjnls_full.gif Related Articles Bacterial cell wall composition and the influence of antibiotics by cell-wall and whole-cell NMR.
Philos Trans R Soc Lond B Biol Sci. 2015 Oct 5;370(1679)
Authors: Romaniuk JA, Cegelski L
Abstract
The ability to characterize bacterial cell-wall composition and structure is...
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07-07-2016 05:58 PM
[NMR paper] Spectral snapshots of bacterial cell-wall composition and the influence of antibiotics by whole-cell NMR.
Spectral snapshots of bacterial cell-wall composition and the influence of antibiotics by whole-cell NMR.
Spectral snapshots of bacterial cell-wall composition and the influence of antibiotics by whole-cell NMR.
Biophys J. 2015 Mar 24;108(6):1380-9
Authors: Nygaard R, Romaniuk JA, Rice DM, Cegelski L
Abstract
Gram-positive bacteria surround themselves with a thick cell wall that is essential to cell survival and is a major target of antibiotics. Quantifying alterations in cell-wall composition are crucial to evaluating drug...
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03-27-2015 11:59 PM
[NMR paper] Real-Time Monitoring of Cancer Cell Metabolism and Effects of an Anticancer Agent using 2D In-Cell NMR Spectroscopy.
Real-Time Monitoring of Cancer Cell Metabolism and Effects of an Anticancer Agent using 2D In-Cell NMR Spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary_FullTextOnline_120x27.gif Related Articles Real-Time Monitoring of Cancer Cell Metabolism and Effects of an Anticancer Agent using 2D In-Cell NMR Spectroscopy.
Angew Chem Int Ed Engl. 2015 Mar 5;
Authors: Wen H, An YJ, Xu WJ, Kang KW, Park S
Abstract
Altered metabolism is a critical part of...
In-cell NMR: Why and how?
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