In-cell NMR [Commentary]

		BioNMR > Educational resources > Journal club
In-cell NMR [Commentary]

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

03-27-2012, 08:41 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,733

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

In-cell NMR [Commentary]

In-cell NMR [Commentary]

Pielak, G. J., Tian, F....
Date: 2012-03-27

In PNAS, Renault et al. (1) describe an advance that will help reveal the atomic details of membrane proteins in living cells. Membrane proteins comprise one-third of all cellular proteins, including biologically crucial molecules such as ion channels, G protein-coupled receptors (GPCRs), and transporters. The GPCR superfamily alone comprises nearly half of our current drug targets. Despite their importance and prevalence, relatively few membrane protein structures are known. Of the more than 20,000 unique high-resolution protein structures in the Protein Data Bank (www.pdb.org) (2), less than 0.5% are membrane proteins. The paucity of membrane protein structures reflects difficulties in preparing... Read More

PNAS:
Number: 13
Volume: 109

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
In-cell NMR spectroscopy In-cell NMR spectroscopy Publication year: 2011 Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 59, Issue 3</br> Andres Y. Maldonado, David S. Burz, Alexander Shekhtman</br> </br> </br></br>	nmrlearner	Journal club	0	03-09-2012 09:16 AM
Anin situelectrochemical cell for Q- and W-band EPR spectroscopy Anin situelectrochemical cell for Q- and W-band EPR spectroscopy Publication year: 2011 Source: Journal of Magnetic Resonance, Available online 22 September 2011</br> Paul R.Murray, DavidCollison, SimonDaff, NicolaAustin, Ruth*Edge, ...</br> A simple design for anin situ, three-electrode spectroelectrochemical cell is reported that can be used in commercial Q- and W-band (ca. 34 and 94 GHz, respectively) electron paramagnetic resonance (EPR) spectrometers, using standard sample tubing (1.0 and 0.5 mm inner diameter, respectively) and within variable temperature cryostat systems....	nmrlearner	Journal club	0	09-24-2011 06:04 AM
[NMR paper] NMR structure and regulated expression in APL cell of human SH3BGRL3. NMR structure and regulated expression in APL cell of human SH3BGRL3. Related Articles NMR structure and regulated expression in APL cell of human SH3BGRL3. FEBS Lett. 2005 May 23;579(13):2788-94 Authors: Xu C, Zheng P, Shen S, Xu Y, Wei L, Gao H, Wang S, Zhu C, Tang Y, Wu J, Zhang Q, Shi Y SH3 domain binding glutamic acid-rich protein like 3 (SH3BGRL3) is the new member of thioredoxin (TRX) super family, whose posttranslational modified form was identified as tumor necrosis factor alpha (TNF-alpha) inhibitory protein, TIP-B1. In this paper,...	nmrlearner	Journal club	0	11-25-2010 08:21 PM
[NMR paper] In-cell NMR spectroscopy. In-cell NMR spectroscopy. Related Articles In-cell NMR spectroscopy. Methods Enzymol. 2005;394:17-41 Authors: Serber Z, Corsini L, Durst F, Dötsch V The role of a protein inside a cell is determined by both its location and its conformational state. Although fluorescence techniques are widely used to determine the cellular localization of proteins in vivo, these approaches cannot provide detailed information about a protein's three-dimensional state. This gap, however, can be filled by NMR spectroscopy, which can be used to investigate both...	nmrlearner	Journal club	0	11-24-2010 11:14 PM
In-Cell NMR Spectroscopy In-Cell NMR Spectroscopy Publication year: 2010 Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 17 November 2010</br> Andres Y., Maldonado , David S., Burz , Alexander, Shekhtman</br> More...	nmrlearner	Journal club	0	11-18-2010 06:16 PM
[BMNRC community] In-cell NMR: three-dimensional protein structures In-cell NMR: three-dimensional protein structures http://www.nature.com/nature/journal/v458/n7234/edsumm/e090305-13.html Go to BMNRC community to find more info about this topic.	nmrlearner	News from other NMR forums	0	08-27-2010 07:58 PM
[KPWU blog] [IDP] Cytoplasmic Domain of the T Cell Receptor zeta Cytoplasmic Domain of the T Cell Receptor zeta Title:* The Intrinsically Disordered Cytoplasmic Domain of the T Cell Receptor ? Chain Binds to the Nef Protein of Simian Immunodeficiency Virus without a Disorder-to-Order Transition Authors: Alexander B. Sigalov, Walter M. Kim, Maria Saline and Lawrence J. Stern Journal: Biochemistry, 2008, 47 (49), pp 12942–12944 Not fully labeled in HSQChttp://stats.wordpress.com/b.gif?host=kpwu.wordpress.com&blog=76132&post=197&subd=kpwu&ref=&feed=1 Go to KPWU blog to read complete post.	nmrlearner	News from NMR blogs	0	08-22-2010 01:33 AM
[Nature network NMR forum] New in-cell NMR group (0 replies) New in-cell NMR group (0 replies) There is a new in-cell NMR group that has just started and needs new members for discussions, etc Here is the link More...	nmrlearner	News from other NMR forums	0	08-21-2010 03:29 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off