Related ArticlesCell Metabolic Profiling of Colorectal Cancer via 1H NMR.
Clin Chim Acta. 2020 Jul 21;:
Authors: Ge S, Zhang Q, Tian Y, Hao L, Duan J, Zhang B
Abstract
BACKGROUND: Protein arginine methyltransferase 5 (PRMT5) belongs to a large family of protein arginine methyltransferases (PRMTs) that play essential role in gene transcription and regulate tumorigenesis. However, the role of PRMT5 in the regulation of cancer cell metabolism remains unclear.
METHODS: Cell metabolomic analysis was performed on SW480 cells transfected with small interfering RNA (siRNA) specifically targeting PRMT5, followed by metabolomic pathway analysis.
RESULTS: PRMT5 was overexpressed in colorectal cancer (CRC) tissues, and downregulation of PRMT5 suppressed CRC cell proliferation and the levels of PRMT5 and symmetric dimethylation of histone H3 (H3R8me2s). In addition, we found distinct differences in metabolite classification and function in PRMT5 knockdown SW480 cells compared to control SW480 cells. PRMT5 knockdown increased the levels of amino acids and carbohydrates, particularly related to the arginine metabolism such as glutamate, glutamine (Gln), proline, creatine, creatinine and phosphocreatine (PCr).
CONCLUSIONS: These findings revealed a key role for PRMT5 as a regulator of CRC cell metabolism to mediate arginine methylation in CRC cells.
PMID: 32707041 [PubMed - as supplied by publisher]
[NMR paper] Anti-cancer effects of emodin on HepG2 cells as revealed by 1H-NMR based metabolic profiling.
Anti-cancer effects of emodin on HepG2 cells as revealed by 1H-NMR based metabolic profiling.
Related Articles Anti-cancer effects of emodin on HepG2 cells as revealed by 1H-NMR based metabolic profiling.
J Proteome Res. 2018 Apr 20;:
Authors: Xing YX, Li MH, Tao L, Ruan LY, Hong W, Chen C, Zhao WL, Xu H, Chen JF, Wang JS
Abstract
Hepatic carcinoma is one of the most common cancers in the world with a high incidence. Emodin is an anthraquinone derived from Polygonum multiflorum Thunb, possessing anti-cancer activity. The purpose...
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04-21-2018 10:14 AM
[NMR paper] Impact of chemotherapy on metabolic reprogramming: Characterization of the metabolic profile of breast cancer MDA-MB-231 cells using (1)H HR-MAS NMR spectroscopy.
Impact of chemotherapy on metabolic reprogramming: Characterization of the metabolic profile of breast cancer MDA-MB-231 cells using (1)H HR-MAS NMR spectroscopy.
Related Articles Impact of chemotherapy on metabolic reprogramming: Characterization of the metabolic profile of breast cancer MDA-MB-231 cells using (1)H HR-MAS NMR spectroscopy.
J Pharm Biomed Anal. 2017 Sep 12;146:324-328
Authors: Maria RM, Altei WF, Selistre-de-Araujo HS, Colnago LA
Abstract
Doxorubicin, cisplatin, and tamoxifen are part of many chemotherapeutic...
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09-16-2017 09:58 PM
[NMR paper] Profiling sulfation/epimerization pattern of full-length heparan sulfate by NMR following cell culture 13C-glucose metabolic labeling.
Profiling sulfation/epimerization pattern of full-length heparan sulfate by NMR following cell culture 13C-glucose metabolic labeling.
Related Articles Profiling sulfation/epimerization pattern of full-length heparan sulfate by NMR following cell culture 13C-glucose metabolic labeling.
Glycobiology. 2014 Oct 21;
Authors: Pegeot M, Sadir R, Eriksson I, Kjellen L, Simorre JP, Gans P, Lortat-Jacob H
Abstract
Through its ability to interact with proteins, heparan sulfate (HS) fulfills a large variety of functions. Protein binding...
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10-23-2014 05:27 PM
[NMR paper] Malignancy-associated metabolic profiling of human glioma cell lines using 1H NMR spectroscopy.
Malignancy-associated metabolic profiling of human glioma cell lines using 1H NMR spectroscopy.
Related Articles Malignancy-associated metabolic profiling of human glioma cell lines using 1H NMR spectroscopy.
Mol Cancer. 2014 Aug 27;13(1):197
Authors: Shao W, Gu J, Huang C, Liu D, Huang H, Huang Z, Lin Z, Yang W, Liu K, Lin D, Ji T
Abstract
BACKGROUND: Ambiguity in malignant transformation of glioma has made prognostic diagnosis very challenging. Tumor malignant transformation is closely correlated with specific alterations...
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08-29-2014 05:36 PM
Colon cancer calculated: Metabolic profiling
Colon cancer calculated: Metabolic profiling
http://www.spectroscopynow.com/common/images/thumbnails/14077d20717.jpgNuclear magnetic resonance (NMR) spectroscopy can be used to obtain a metabolic fingerprint of cancer of the bowel and so determine how far advanced the disease is, according to UK researchers.
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08-15-2013 07:22 AM
[NMR paper] Quantitative NMR analysis of Erk activity and inhibition by U0126 in a panel of patient-derived colorectal cancer cell lines.
Quantitative NMR analysis of Erk activity and inhibition by U0126 in a panel of patient-derived colorectal cancer cell lines.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Quantitative NMR analysis of Erk activity and inhibition by U0126 in a panel of patient-derived colorectal cancer cell lines.
Biochim Biophys Acta. 2013 Jan 26;
Authors: Rose HM, Stuiver M, Thongwichian R, Theillet FX, Feller SM, Selenko P
Abstract
We comparatively analyzed the basal activity...
Cell Metabolic Profiling of Colorectal Cancer via 1H NMR.
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