Carbohydrates are essential to various life activities in living organisms and serve as the central component in many biomaterials. As an emerging technique with steadily improving resolution, solid-state Nuclear Magnetic Resonance (NMR) spectroscopy has the unique capability in revealing the polymorphic structure and heterogeneous dynamics of insoluble complex carbohydrates. Here, we report the first solid-state NMR database for complex carbohydrates, Complex Carbohydrates Magnetic Resonance Database (CCMRD). This database currently holds the chemical shift information of more than four hundred solid-state NMR compounds and expects rapid expansion. CCMRD provides open portals for data deposition and supports search options based on NMR chemical shifts, carbohydrate names, and compound classes. With the timely implementation, this platform will facilitate spectral analysis and structure determination of carbohydrates and promote software development to benefit the research community. The database is freely accessible at www.ccmrd.org.
(3, 2)D 1 H, 13 C BIRD r,X -HSQC-TOCSY for NMR structure elucidation of mixtures: application to complex carbohydrates
(3, 2)D 1 H, 13 C BIRD r,X -HSQC-TOCSY for NMR structure elucidation of mixtures: application to complex carbohydrates
Abstract
Overlap of NMR signals is the major cause of difficulties associated with NMR structure elucidation of molecules contained in complex mixtures. A 2D homonuclear correlation spectroscopy in particular suffers from low dispersion of 1H chemical shifts; larger dispersion of 13C chemical shifts is often used to reduce this overlap, while still providing the protonâ??proton correlation information e.g. in the form of a 2D 1H, 13C...
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01-11-2018 04:07 PM
[NMR paper] (2)H-(13)C correlation solid-state NMR for investigating dynamics and water accessibilities of proteins and carbohydrates.
(2)H-(13)C correlation solid-state NMR for investigating dynamics and water accessibilities of proteins and carbohydrates.
(2)H-(13)C correlation solid-state NMR for investigating dynamics and water accessibilities of proteins and carbohydrates.
J Biomol NMR. 2017 Jul 03;:
Authors: Gelenter MD, Wang T, Liao SY, O'Neill H, Hong M
Abstract
Site-specific determination of molecular motion and water accessibility by indirect detection of (2)H NMR spectra has advantages over dipolar-coupling based techniques due to the large...
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07-05-2017 10:27 AM
2 Hâ?? 13 C correlation solid-state NMR for investigating dynamics and water accessibilities of proteins and carbohydrates
2 Hâ?? 13 C correlation solid-state NMR for investigating dynamics and water accessibilities of proteins and carbohydrates
Abstract
Site-specific determination of molecular motion and water accessibility by indirect detection of 2H NMR spectra has advantages over dipolar-coupling based techniques due to the large quadrupolar couplings and the ensuing high angular resolution. Recently, a Rotor Echo Short Pulse IRrAdiaTION mediated cross polarization (RESPIRATIONCP) technique was developed, which allowed efficient transfer of 2H magnetization to 13C at...
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07-03-2017 03:18 PM
[NMR paper] Solid-state NMR of a protein in a precipitated complex with a full-length antibody.
Solid-state NMR of a protein in a precipitated complex with a full-length antibody.
Solid-state NMR of a protein in a precipitated complex with a full-length antibody.
J Am Chem Soc. 2014 Nov 10;
Authors: Lamley JM, Iuga D, Oster C, Sass HJ, Rogowski M, Oss A, Past J, Reinhold A, Grzesiek S, Samoson A, Lewandowski JR
Abstract
NMR is a prime technique for characterizing atomic resolution structures and dynamics of biomolecular complexes but, for such systems, faces challenges of sensitivity and spectral resolution. We...
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11-11-2014 11:57 AM
[NMR paper] Solid-State NMR Study of a 41 kDa Membrane Protein Complex DsbA/DsbB.
Solid-State NMR Study of a 41 kDa Membrane Protein Complex DsbA/DsbB.
Related Articles Solid-State NMR Study of a 41 kDa Membrane Protein Complex DsbA/DsbB.
J Phys Chem B. 2013 Mar 25;
Authors: Sperling LJ, Tang M, Berthold DA, Nesbitt AE, Gennis RB, Rienstra CM
Abstract
The disulfide bond generation system in E. coli is led by a periplasmic protein DsbA and an integral membrane protein DsbB. Here we present a solid-state NMR (SSNMR) study of a 41 kDa membrane protein complex DsbA/DsbB precipitated in the presence of native lipids to...
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03-27-2013 03:33 PM
Solid-state NMR spectroscopy on complex biomolecules.
Solid-state NMR spectroscopy on complex biomolecules.
Solid-state NMR spectroscopy on complex biomolecules.
Angew Chem Int Ed Engl. 2010 Nov 2;49(45):8346-57
Authors: Renault M, Cukkemane A, Baldus M
Biomolecular applications of NMR spectroscopy are often merely associated with soluble molecules or magnetic resonance imaging. However, since the late 1970s, solid-state NMR (ssNMR) spectroscopy has demonstrated its ability to provide atomic-level insight into complex biomolecular systems ranging from lipid bilayers to complex biomaterials. In the...
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02-15-2011 07:17 PM
[NMR paper] NMR and molecular modeling of complex carbohydrates and carbohydrate-protein interact
NMR and molecular modeling of complex carbohydrates and carbohydrate-protein interactions. Applications to anti-bacteria vaccines.
Related Articles NMR and molecular modeling of complex carbohydrates and carbohydrate-protein interactions. Applications to anti-bacteria vaccines.
Adv Exp Med Biol. 2001;491:543-50
Authors: Brisson JR, Jennings HJ
In order to characterize the conformational epitope of the group B meningococcal polysaccharide and of the type III group B Streptococcus capsular polysaccharide NMR measurements were done on a wide...
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11-19-2010 08:32 PM
[NMR paper] Solid state 15N NMR evidence for a complex Schiff base counterion in the visual G-pro
Solid state 15N NMR evidence for a complex Schiff base counterion in the visual G-protein-coupled receptor rhodopsin.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles Solid state 15N NMR evidence for a complex Schiff base counterion in the visual G-protein-coupled receptor rhodopsin.
Biochemistry. 1999 Jun 1;38(22):7195-9
Authors: Creemers AF, Klaassen CH, Bovee-Geurts PH, Kelle R, Kragl U, Raap J, de Grip WJ, Lugtenburg J, de Groot HJ
Using the baculovirus/Sf9 cell expression system, we...
CCMRD: a solid-state NMR database for complex carbohydrates
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