Related ArticlesCapillary array electrophoretic NMR of proteins in biological buffer solutions.
J Magn Reson. 1999 Dec;141(2):355-9
Authors: He Q, Liu Y, Sun H, Li E
The capillary array electrophoretic NMR (CA-ENMR) was developed to study protein mixtures in biological buffer solutions of high ionic strength. By enhancing the strength of the effective electric field across the sample, the technique permits the detection of the electrophoretic motion of 1 mM lysozyme in 50 mM NaH(2)PO(4) aqueous solution, which was previously not achievable using conventional ENMR. Heat-induced convection was dramatically reduced by blocking convective current loops and by improving the efficiency of heat exchange. Thus, the capability of ENMR study of electrolyte solutions was extended from low to high ionic strength. In addition, capillary walls reduced rf-induced electrical eddy current, thereby maintaining good probe Q factors. Because of its parallel configuration to the static magnetic field, the capillary array chamber produced no susceptibility distortions of the ENMR signal. The technique offers great potential in characterizing multiple protein conformations and protein interactions in solution.
[NMR paper] Determination of regulatory phosphorylation sites in nanogram amounts of a synthetic fragment of ZAP-70 using microprobe NMR and on-line coupled capillary HPLC-NMR.
Determination of regulatory phosphorylation sites in nanogram amounts of a synthetic fragment of ZAP-70 using microprobe NMR and on-line coupled capillary HPLC-NMR.
Related Articles Determination of regulatory phosphorylation sites in nanogram amounts of a synthetic fragment of ZAP-70 using microprobe NMR and on-line coupled capillary HPLC-NMR.
Magn Reson Chem. 2005 Sep;43(9):747-54
Authors: Hentschel P, Krucker M, Grynbaum MD, Putzbach K, Bischoff R, Albert K
The protein kinase ZAP-70 is involved in T-cell activation and interacts with...
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[NMR paper] Trp repressor-operator binding: NMR and electrophoretic mobility shift studies of the
Trp repressor-operator binding: NMR and electrophoretic mobility shift studies of the effect of DNA sequence and corepressor binding on two Trp repressor-operator complexes.
Related Articles Trp repressor-operator binding: NMR and electrophoretic mobility shift studies of the effect of DNA sequence and corepressor binding on two Trp repressor-operator complexes.
Biochemistry. 2002 Dec 17;41(50):14866-78
Authors: Jaseja M, Jeeves M, Hyde EI
In Trp repressor-DNA complexes, most interactions either occur with phosphate groups or are...
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[NMR paper] Constant-time multidimensional electrophoretic NMR.
Constant-time multidimensional electrophoretic NMR.
Related Articles Constant-time multidimensional electrophoretic NMR.
J Magn Reson. 2002 Jun;156(2):181-6
Authors: Li E, He Q
Multidimensional electrophoretic NMR (ENMR) has been introduced to determine structures of coexisting proteins and protein conformations in solution. Signals of different proteins are separated in a new dimension of electrophoretic flow according to their characteristic electrophoretic mobilities. The electrophoretic interferograms have been generated in the flow...
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[NMR paper] Convection compensated electrophoretic NMR.
Convection compensated electrophoretic NMR.
Related Articles Convection compensated electrophoretic NMR.
J Magn Reson. 2001 Jun;150(2):126-31
Authors: He Q, Wei Z
A novel method of convection compensated ENMR (CC-ENMR) has been developed to detect electrophoretic motion of ionic species in the presence of bulk solution convection. This was accomplished using a gradient moment nulling technique to remove spectral artifacts from heat-induced convection and using the polarity switch of the applied electric field to retain spin phase modulations...
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11-19-2010 08:32 PM
[NMR paper] Three-dimensional electrophoretic NMR correlation spectroscopy.
Three-dimensional electrophoretic NMR correlation spectroscopy.
Related Articles Three-dimensional electrophoretic NMR correlation spectroscopy.
J Magn Reson. 2000 Dec;147(2):361-5
Authors: He Q, Lin W, Liu Y, Li E
A novel method of three-dimensional electrophoretic NMR correlation spectroscopy (3D EP-COSY) has been proposed, developed, and implemented. It has a demonstrated potential of facilitating simultaneous structural assignments of multiple proteins in mixtures. The principle is to add a pulsed DC electric field that introduces a new...
[NMR paper] The hydration of proteins in solutions by self-diffusion coefficients. NMR study.
The hydration of proteins in solutions by self-diffusion coefficients. NMR study.
Related Articles The hydration of proteins in solutions by self-diffusion coefficients. NMR study.
Biochim Biophys Acta. 1996 Apr 17;1289(3):312-4
Authors: Baranowska HM, Olszewski KJ
The hydration of the globular (lysozyme, albumin) and fibrillar (fibrinogen) proteins in solution has been determined from the measurements of the self-diffusion coefficient by NMR pulsed gradient method. It has been concluded that the concentration dependencies of the...
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[NMR paper] NMR spectroscopy of hydroxyl protons in aqueous solutions of peptides and proteins.
NMR spectroscopy of hydroxyl protons in aqueous solutions of peptides and proteins.
Related Articles NMR spectroscopy of hydroxyl protons in aqueous solutions of peptides and proteins.
J Biomol NMR. 1992 Sep;2(5):447-65
Authors: Liepinsh E, Otting G, Wüthrich K
Hydroxyl groups of serine and threonine, and to some extent also tyrosine are usually located on or near the surface of proteins. NMR observations of the hydroxyl protons is therefore of interest to support investigations of the protein surface in solution, and knowledge of the...
Capillary array electrophoretic NMR of proteins in biological buffer solutions.
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