Related ArticlesCalmodulin discriminates between the two enantiomers of the receptor-operated calcium channel blocker SK&F 96365: a study using 1H-NMR and chiral HPLC.
Chirality. 1990;2(4):229-32
Authors: Reid DG, MacLachlan LK, Robinson SP, Camilleri P, Dyke CA, Thorpe CJ
1H nuclear magnetic resonance at 360 MHz shows that SK&F 96365 (1-(beta-[3-(p-methoxyphenyl)-propyloxy]-p-methoxyphenethyl)-1H- imidazole hydrochloride), an antagonist of mammalian receptor-operated calcium channels, interacts with the calcium-binding regulatory protein calmodulin (CaM). This may be inferred by a number of chemical shift changes in the spectrum of the calcium-saturated protein induced by addition of the compound. Moreover, two well-resolved singlets corresponding to the 2-proton of the SK&F 96365 imidazolium moiety are observed in the spectrum over a wide range of protein:compound ratios. Separation of rac SK&F 96365 into its two enantiomers by high-performance liquid chromatography on a cellulose tris (4-methylbenzoate) column enabled us to show that the doubling of this NMR signal in the presence of CaM is due to a propensity of the protein to distinguish between the two optical isomers of the compound.
[NMR images] Bruker 500 MHz NMR operated by
http://www.bama.ua.edu/~chem/facilities/instruments/bruker_500.jpg
bama.ua.edu
27/10/2011 11:34:04 AM GMT
Bruker 500 MHz NMR operated by
More...
nmrlearner
NMR pictures
0
11-03-2011 02:52 PM
Naturally occurring biodegradable polymers as the basis of chiral gels for the distinction of enantiomers by partially oriented NMR spectroscopy.
Naturally occurring biodegradable polymers as the basis of chiral gels for the distinction of enantiomers by partially oriented NMR spectroscopy.
Naturally occurring biodegradable polymers as the basis of chiral gels for the distinction of enantiomers by partially oriented NMR spectroscopy.
Int J Artif Organs. 2011 Feb;34(2):134-8
Authors: Büchler SS, Kummerlöwe G, Luy B
In modern, high resolution NMR spectroscopy, anisotropic parameters play an important role. They can be measured with the help of liquid crystalline mesophases or stretched...
nmrlearner
Journal club
0
07-14-2011 04:40 PM
NMR structural study of the intracellular loop 3 of the serotonin 5-HT(1A) receptor and its interaction with calmodulin.
NMR structural study of the intracellular loop 3 of the serotonin 5-HT(1A) receptor and its interaction with calmodulin.
NMR structural study of the intracellular loop 3 of the serotonin 5-HT(1A) receptor and its interaction with calmodulin.
Biochim Biophys Acta. 2011 May 24;
Authors: Chen AS, Kim YM, Gayen S, Huang Q, Raida M, Kang C
The serotonin (5-HT(1A)) receptor, a G-protein-coupled receptor (GPCR), plays important roles in serotonergic signaling in the central nervous system. The third intracellular loop (ICL3) of the 5-HT(1A) receptor has...
Fast methionine-based solution structure determination of calcium-calmodulin complexes
Fast methionine-based solution structure determination of calcium-calmodulin complexes
Abstract Here we present a novel NMR method for the structure determination of calcium-calmodulin (Ca2+-CaM)-peptide complexes from a limited set of experimental restraints. A comparison of solved CaM-peptide structures reveals invariability in CaMâ??s backbone conformation and a structural plasticity in CaMâ??s domain orientation enabled by a flexible linker. Knowing this, the collection and analysis of an extensive set of NOESY spectra is redundant. Although RDCs can define CaM domain orientation in...
nmrlearner
Journal club
0
03-03-2011 02:06 AM
[NMR paper] Calcium-induced refolding of the calmodulin V136G mutant studied by NMR spectroscopy:
Calcium-induced refolding of the calmodulin V136G mutant studied by NMR spectroscopy: evidence for interaction between the two globular domains.
Related Articles Calcium-induced refolding of the calmodulin V136G mutant studied by NMR spectroscopy: evidence for interaction between the two globular domains.
Biochemistry. 2000 Dec 26;39(51):15920-31
Authors: Fefeu S, Biekofsky RR, McCormick JE, Martin SR, Bayley PM, Feeney J
The Ca(2+) titration of the (15)N-labeled mutant V136G calmodulin has been monitored using (1)H-(15)N HSQC NMR spectra. Up...
nmrlearner
Journal club
0
11-19-2010 08:29 PM
[NMR paper] Synthesis and analysis of the enantiomers of calmidazolium, and a 1H NMR demonstratio
Synthesis and analysis of the enantiomers of calmidazolium, and a 1H NMR demonstration of a chiral interaction with calmodulin.
Related Articles Synthesis and analysis of the enantiomers of calmidazolium, and a 1H NMR demonstration of a chiral interaction with calmodulin.
Chirality. 1996;8(8):545-50
Authors: Edwards AJ, Sweeney PJ, Reid DG, Walker JM, Elshourbagy N, Egwuagu CE, Young JF, Patton CL
Calmidazolium -3-ethyl]-1H-imidazolium chloride] is a potent calmodulin inhibitor. This paper describes the synthesis and properties of the...
nmrlearner
Journal club
0
08-22-2010 02:27 PM
[NMR paper] Rotational dynamics of calcium-free calmodulin studied by 15N-NMR relaxation measurem
Rotational dynamics of calcium-free calmodulin studied by 15N-NMR relaxation measurements.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Rotational dynamics of calcium-free calmodulin studied by 15N-NMR relaxation measurements.
Eur J Biochem. 1995 Jun 15;230(3):1014-24
Authors: Tjandra N, Kuboniwa H, Ren H, Bax A
The backbone motions of calcium-free Xenopus calmodulin have been characterized by measurements of the 15N...
Calmodulin discriminates between the two enantiomers of the receptor-operated calcium
Linear Mode
