CACA-TOCSY with alternate 13Câ??12C labeling: a 13Cα direct detection experiment for
Abstract We present a 13C direct detection CACA-TOCSY experiment for samples with alternate 13Câ??12C labeling. It provides inter-residue correlations between 13Cα resonances of residue i and adjacent Cαs at positions i â?? 1 and i + 1. Furthermore, longer mixing times yield correlations to Cα nuclei separated by more than one residue. The experiment also provides Cα-to-sidechain correlations, some amino acid type identifications and estimates for Ï? dihedral angles. The power of the experiment derives from the alternate 13Câ??12C labeling with [1,3-13C] glycerol or [2-13C] glycerol, which allows utilizing the small scalar 3JCC couplings that are masked by strong 1JCC couplings in uniformly 13C labeled samples.
Content Type Journal Article
DOI 10.1007/s10858-010-9410-3
Authors
Koh Takeuchi, Harvard Medical School Department of Biological Chemistry and Molecular Pharmacology 240 Longwood Avenue Boston MA 02115 USA
Dominique P. Frueh, Harvard Medical School Department of Biological Chemistry and Molecular Pharmacology 240 Longwood Avenue Boston MA 02115 USA
Zhen-Yu J. Sun, Harvard Medical School Department of Biological Chemistry and Molecular Pharmacology 240 Longwood Avenue Boston MA 02115 USA
Sebastian Hiller, Harvard Medical School Department of Biological Chemistry and Molecular Pharmacology 240 Longwood Avenue Boston MA 02115 USA
Gerhard Wagner, Harvard Medical School Department of Biological Chemistry and Molecular Pharmacology 240 Longwood Avenue Boston MA 02115 USA
Speeding up direct 15N detection: hCaN 2D NMR experiment
Speeding up direct 15N detection: hCaN 2D NMR experiment
Abstract Experiments detecting low gyromagnetic nuclei have recently been proposed to utilize the relatively slow relaxation properties of these nuclei in comparison to 1H. Here we present a new type of 15N direct-detection experiment. Like the previously proposed CaN experiment (Takeuchi et al. in J Biomol NMR 47:271â??282, 2010), the hCaN experiment described here sequentially connects amide 15N resonances, but utilizes the initial high polarization and the faster recovery of the 1H nucleus to shorten the recycling delay. This...
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11-14-2011 08:45 AM
13C direct-detection biomolecular NMR spectroscopy in living cells.
13C direct-detection biomolecular NMR spectroscopy in living cells.
13C direct-detection biomolecular NMR spectroscopy in living cells.
Angew Chem Int Ed Engl. 2011 Mar 1;50(10):2339-41
Authors: Bertini I, Felli IC, Gonnelli L, Kumar M V V, Pierattelli R
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06-18-2011 01:10 PM
Direct Detection of Nitrogen-14 in Solid-State NMR Spectroscopy
Direct Detection of Nitrogen-14 in Solid-State NMR Spectroscopy
Publication year: 2011
Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 20 April 2011</br>
Luke A., O’Dell</br>
*Highlights:*? Experimental methods for the direct detection of 14N are surveyed ? Advantages, disadvantages and practicalities of each technique are discussed ? Includes single-crystal, ultra-wideline, MAS and overtone spectroscopy</br></br>
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04-21-2011 03:00 PM
HNCA-TOCSY-CANH experiments with alternate 13C-12C labeling: a set of 3D experiment w
HNCA-TOCSY-CANH experiments with alternate 13C-12C labeling: a set of 3D experiment with unique supra-sequential information for mainchain resonance assignment
Abstract Described here is a set of three-dimensional (3D) NMR experiments that rely on CACA-TOCSY magnetization transfer via the weak
3 \textJ\textCa\textCa coupling. These pulse sequences, which resemble recently described 13C detected CACA-TOCSY (Takeuchi et al. 2010) experiments, are recorded in 1H2O, and use 1H excitation and detection. These experiments require alternate 13C-12C labeling together with perdeuteration,...
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11-27-2010 07:34 PM
[Question from NMRWiki Q&A forum] How to set up 15N direct detection on UnityInova?
How to set up 15N direct detection on UnityInova?
Has anyone recorded directly detected 15N spectra on UnityInova? We have a triple resonance HCN probe. The sample I've used was natural abundance 1M NaNO3 - sodium nitrate.
I've tried to set up cable analogously to 13C detection - through the second preamp, but could not see any signal at all.
Check if somebody has answered this question on NMRWiki QA forum
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08-22-2010 02:30 AM
[Question from NMRWiki Q&A forum] What has been done with direct detection of Deuterium?
What has been done with direct detection of Deuterium?
Hello, I am trying to investigate what has been done in NMR using direct detection of deuterium in liquids. - Could you suggest any citations?
What kind of sensitivity gain there is in using broadband probe vs. lock channel?
Has anything been done/can be done to detect scalar couplings with protons like in COSY or TOCSY? Are HD couplings other than geminal detectable?
Thanks.
Simultaneous detection of amide and methyl correlations using a time shared NMR experiment: application to binding epitope mapping
Simultaneous detection of amide and methyl correlations using a time shared NMR experiment: application to binding epitope mapping
Peter Würtz, Olli Aitio, Maarit Hellman and Perttu Permi
Journal of Biomolecular NMR; 2007; 39(2) pp 97 - 105
Abstract:
Simultaneous recording of different NMR parameters is an efficient way to reduce the overall experimental time and speed up structural studies of biological macromolecules. This can especially be beneficial in the case of fast NMR-based drug screening applications or for collecting NOE restraints, where prohibitively long data collection...
CACA-TOCSY with alternate 13Câ??12C labeling: a 13Cα direct detection experiment for
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