[NMR paper] The C-terminal cytidine deaminase domain of APOBEC3G itself undergoes intersegmental transfer for a target search, as revealed by real-time NMR monitoring.
The C-terminal cytidine deaminase domain of APOBEC3G itself undergoes intersegmental transfer for a target search, as revealed by real-time NMR monitoring.
Related ArticlesThe C-terminal cytidine deaminase domain of APOBEC3G itself undergoes intersegmental transfer for a target search, as revealed by real-time NMR monitoring.
Phys Chem Chem Phys. 2018 Jan 31;20(5):2976-2981
Authors: Kamba K, Nagata T, Katahira M
Abstract
APOBEC3G (A3G), an anti-human immunodeficiency virus 1 factor, deaminates cytidines. We examined deamination of two cytidines located separately on substrate ssDNA by the C-terminal domain (CTD) of A3G using real-time NMR monitoring. The deamination preference between the two cytidines was lost when either the substrate or non-substrate ssDNA concentration increased. When the non-substrate ssDNA concentration increased, the deamination activity first increased, but then decreased. This indicates that even a single domain, A3G-CTD, undergoes intersegmental transfer for a target search.
Hyperpolarized 133Cs is a sensitive probe for real-time monitoring of biophysical environments
From The DNP-NMR Blog:
Hyperpolarized 133Cs is a sensitive probe for real-time monitoring of biophysical environments
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Karlsson, M., J.H. Ardenkjaer-Larsen, and M.H. Lerche, Hyperpolarized 133Cs is a sensitive probe for real-time monitoring of biophysical environments. Chemical Communications, 2017. 53(49): p. 6625-6628.
http://dx.doi.org/10.1039/C7CC02943H
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[NMR paper] In vivo NMR spectroscopy: toward real time monitoring of environmental stress.
In vivo NMR spectroscopy: toward real time monitoring of environmental stress.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-7315-19-Wiley_FullText_120x30_orange.png Related Articles In vivo NMR spectroscopy: toward real time monitoring of environmental stress.
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PMID: 25296400
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[NMR paper] Catalytic analysis of APOBEC3G involving real-time NMR spectroscopy reveals nucleic acid determinants for deamination.
Catalytic analysis of APOBEC3G involving real-time NMR spectroscopy reveals nucleic acid determinants for deamination.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.plosone.org-images-pone_120x30.png http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.ncbi.nlm.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Catalytic analysis of APOBEC3G involving real-time NMR spectroscopy reveals nucleic acid determinants for deamination.
PLoS One. 2015;10(4):e0124142
Authors: Kamba K, Nagata T,...
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Real-time NMR monitoring of biological activities in complex physiological environments.
Curr Opin Struct Biol. 2015 Feb 26;32C:39-47
Authors: Smith MJ, Marshall CB, Theillet FX, Binolfi A, Selenko P, Ikura M
Abstract
Biological reactions occur in a highly organized spatiotemporal context and with kinetics that are modulated by multiple environmental factors. To integrate these variables in our experimental investigations of 'native'...
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[NMR paper] Quantitative analysis of location- and sequence-dependent deamination by APOBEC3G using real-time NMR spectroscopy.
Quantitative analysis of location- and sequence-dependent deamination by APOBEC3G using real-time NMR spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary-Button_120x27px_FullText.gif Related Articles Quantitative analysis of location- and sequence-dependent deamination by APOBEC3G using real-time NMR spectroscopy.
Angew Chem Int Ed Engl. 2014 Feb 24;53(9):2349-52
Authors: Furukawa A, Sugase K, Morishita R, Nagata T, Kodaki T, Takaori-Kondo A, Ryo A, Katahira M
...
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Monitoring Mechanistic Details in the Synthesis of Pyrimidines via Real-Time, Ultrafast Multidimensional NMR Spectroscopy
Monitoring Mechanistic Details in the Synthesis of Pyrimidines via Real-Time, Ultrafast Multidimensional NMR Spectroscopy
Zulay D. Pardo, Gregory L. Olsen, Mari?a Encarnacio?n Ferna?ndez-Valle, Lucio Frydman, Roberto Marti?nez-A?lvarez and Antonio Herrera
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja210154g/aop/images/medium/ja-2011-10154g_0006.gif
Journal of the American Chemical Society
DOI: 10.1021/ja210154g
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/tEFGGh16-DU
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[NMR paper] Real time NMR monitoring of local unfolding of HIV-1 protease tethered dimer driven b
Real time NMR monitoring of local unfolding of HIV-1 protease tethered dimer driven by autolysis.
Related Articles Real time NMR monitoring of local unfolding of HIV-1 protease tethered dimer driven by autolysis.
FEBS Lett. 2001 May 18;497(1):59-64
Authors: Panchal SC, Bhavesh NS, Hosur RV
Structural studies in proteases have been hampered because of their inherent autolytic function. However, since autolysis is known to be mediated via protein unfolding, careful monitoring of the autolytic reaction has the potential to throw light on the...
The C-terminal cytidine deaminase domain of APOBEC3G itself undergoes intersegmental transfer for a target search, as revealed by real-time NMR monitoring.
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