Broadband 15Nâ??13C dipolar recoupling via symmetry-based RF pulse schemes at high MA
Abstract An approach for generating efficient
RNnnS, nk symmetry-based dual channel RF pulse schemes for γ-encoded broadband 15Nâ??13C dipolar recoupling at high magic angle spinning frequencies is presented. The method involves the numerical optimisation of the RF phase-modulation profile of the basic â??Râ?? element so as to obtain heteronuclear double quantum dipolar recoupling sequences with satisfactory magnetisation transfer characteristics. The basic â??Râ?? element was implemented as a sandwich of a small number of short pulses of equal duration with each pulse characterised by a RF phase and amplitude values. The performance characteristics of the sequences were evaluated via numerical simulations and 15Nâ??13C chemical shift correlation experiments. Employing such 13Câ??15N double-quantum recoupling sequences and the multiple receiver capabilities available in the current generation of NMR spectrometers, the possibility to simultaneously acquire 3D NCC and CNH chemical shift correlation spectra is also demonstrated.
Content Type Journal Article
DOI 10.1007/s10858-010-9406-z
Authors
Christian Herbst, Fritz Lipmann Institute Research Group Biomolecular NMR Spectroscopy, Leibniz Institute for Age Research 07745 Jena Germany
Jirada Herbst, Fritz Lipmann Institute Research Group Biomolecular NMR Spectroscopy, Leibniz Institute for Age Research 07745 Jena Germany
Michela Carella, Fritz Lipmann Institute Research Group Biomolecular NMR Spectroscopy, Leibniz Institute for Age Research 07745 Jena Germany
Jörg Leppert, Fritz Lipmann Institute Research Group Biomolecular NMR Spectroscopy, Leibniz Institute for Age Research 07745 Jena Germany
Oliver Ohlenschläger, Fritz Lipmann Institute Research Group Biomolecular NMR Spectroscopy, Leibniz Institute for Age Research 07745 Jena Germany
Matthias Görlach, Fritz Lipmann Institute Research Group Biomolecular NMR Spectroscopy, Leibniz Institute for Age Research 07745 Jena Germany
Ramadurai Ramachandran, Fritz Lipmann Institute Research Group Biomolecular NMR Spectroscopy, Leibniz Institute for Age Research 07745 Jena Germany
High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins
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Abstract Four novel 5D (HACA(N)CONH, HNCOCACB, (HACA)CON(CA)CONH, (H)NCO(NCA)CONH), and one 6D ((H)NCO(N)CACONH) NMR pulse sequences are proposed. The new experiments employ non-uniform sampling that enables achieving high resolution in indirectly detected dimensions. The experiments facilitate resonance assignment of intrinsically disordered proteins. The novel pulse sequences were successfully tested using δ subunit (20 kDa) of Bacillus subtilis RNA polymerase...
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[Question from NMRWiki Q&A forum] Looking for hsqc-based ZZ exchange bruker pulse program
Looking for hsqc-based ZZ exchange bruker pulse program
hi nmrwikier !
Anyone know if the hsqc-based zz exchange pulse prog developed by Farrow et al. (1994) is available on standart bruker pulse prog library and what is its name ?
Many thanks !
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07-06-2011 06:14 AM
Chemical shift correlation at high MAS frequencies employing low-power symmetry-based mixing schemes
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Abstract An approach for conveniently implementing low-power CN n ν and RN n ν symmetry-based band-selective mixing sequences for generating homo- and heteronuclear chemical shift correlation NMR spectra of low γ nuclei in biological solids is demonstrated. Efficient magnetisation transfer characteristics are achieved by selecting appropriate symmetries requiring the application of basic RF elements of relatively long duration and numerically tailoring the RF field modulation profile...
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Radio frequency assisted homonuclear recoupling - A Floquet description of homonuclear recoupling via surrounding heteronuclei in fully protonated to fully deuterated systems
Radio frequency assisted homonuclear recoupling - A Floquet description of homonuclear recoupling via surrounding heteronuclei in fully protonated to fully deuterated systems
Publication year: 2011
Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 18 January 2011</br>
Michal, Leskes , Ümit, Akbey , Hartmut, Oschkinat , Barth-Jan, van Rossum , Shimon, Vega</br>
We present a Floquet theory approach for the analysis of homonuclear recoupling assisted by radio frequency (RF) irradiation of surrounding heteronuclear spins. This description covers a...
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Authors: Wan X, Tegos T, Lin G
In NMR protein structure determination, after the resonance peaks have been identified and chemical shifts from peaks across multiple spectra have been grouped into spin systems, associating these spin systems to their host residues is the key toward the success of structural information extraction and thus the...
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J Chem Phys. 2010 Aug 14;133(6):064501
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Broadband homonuclear TOCSY with amplitude and phase-modulated RF mixing schemes
Broadband homonuclear TOCSY with amplitude and phase-modulated RF mixing schemes
Anika Kirschstein, Christian Herbst, Kerstin Riedel, Michela Carella, Jörg Leppert, Oliver Ohlenschläger, Matthias Görlach and Ramadurai Ramachandran
Journal of Biomolecular NMR; 2008; 40(4); pp 227-237
Abstract:
We have explored the design of broadband scalar coupling mediated 13C–13C and cross-relaxation suppressed 1H–1H TOCSY sequences employing phase/amplitude modulated inversion pulses. Considering a variety of supercycles, pulsewidths and a RF field strength of 10 kHz, the Fourier coefficients...
Broadband 15Nâ??13C dipolar recoupling via symmetry-based RF pulse schemes at high MA
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