?-arrestins (?arrs) play multifaceted roles in the function of G protein-coupled receptors (GPCRs). ?arrs typically interact with phosphorylated C-terminal tail (C tail) and transmembrane core (TM core) of GPCRs. However, the effects of the C tail- and TM core-mediated interactions on the conformational activation of ?arrs have remained elusive. Here, we show the conformational changes for ?arr activation upon the C tail- and TM core-mediated interactions with a prototypical GPCR by nuclear...
[NMR paper] GPCR Activation States Induced by Nanobodies and Mini-G Proteins Compared by NMR Spectroscopy.
GPCR Activation States Induced by Nanobodies and Mini-G Proteins Compared by NMR Spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--www.ncbi.nlm.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.png Related Articles GPCR Activation States Induced by Nanobodies and Mini-G Proteins Compared by NMR Spectroscopy.
Molecules. 2020 Dec 17;25(24):
Authors: Rößler P, Mayer D, Tsai CJ, Veprintsev DB, Schertler GFX, Gossert AD
Abstract
In this work, we examine methyl nuclear magnetic resonance (NMR) spectra of the...
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[NMR paper] Structural Insights into ?-arrestin/CB1 Receptor Interaction: NMR and CD Studies on Model Peptides.
Structural Insights into ?-arrestin/CB1 Receptor Interaction: NMR and CD Studies on Model Peptides.
Related Articles Structural Insights into ?-arrestin/CB1 Receptor Interaction: NMR and CD Studies on Model Peptides.
Int J Mol Sci. 2020 Oct 30;21(21):
Authors: Morales P, Bruix M, Jiménez MA
Abstract
Activation of the cannabinoid CB1 receptor induces different cellular signaling cascades through coupling to different effector proteins (G-proteins and ?-arrestins), triggering numerous therapeutic effects. Conformational changes and...
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[NMR paper] Phosphorylation-induced conformation of ?2-adrenoceptor related to arrestin recruitment revealed by NMR.
Phosphorylation-induced conformation of ?2-adrenoceptor related to arrestin recruitment revealed by NMR.
Related Articles Phosphorylation-induced conformation of ?2-adrenoceptor related to arrestin recruitment revealed by NMR.
Nat Commun. 2018 Jan 15;9(1):194
Authors: Shiraishi Y, Natsume M, Kofuku Y, Imai S, Nakata K, Mizukoshi T, Ueda T, Iwaï H, Shimada I
Abstract
The C-terminal region of G-protein-coupled receptors (GPCRs), stimulated by agonist binding, is phosphorylated by GPCR kinases, and the phosphorylated GPCRs bind to...
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01-18-2018 12:41 PM
CH3] Labeled Proteins for Methyl-TROSY NMR - SelectScience
CH3] Labeled Proteins for Methyl-TROSY NMR - SelectScience
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CH3] Labeled Proteins for Methyl-TROSY NMR
SelectScience
Isotope labelling has revolutionized the use of biomolecular NMR spectroscopy, allowing the exploration of molecular interactions with high sensitivity and resolution. Introducing labelled Thr, a protein often found at molecular interfaces and involved ...
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[NMR paper] Phospho-selective mechanisms of arrestin conformations and functions revealed by unnatural amino acid incorporation and (19)F-NMR.
Phospho-selective mechanisms of arrestin conformations and functions revealed by unnatural amino acid incorporation and (19)F-NMR.
Phospho-selective mechanisms of arrestin conformations and functions revealed by unnatural amino acid incorporation and (19)F-NMR.
Nat Commun. 2015;6:8202
Authors: Yang F, Yu X, Liu C, Qu CX, Gong Z, Liu HD, Li FH, Wang HM, He DF, Yi F, Song C, Tian CL, Xiao KH, Wang JY, Sun JP
Abstract
Specific arrestin conformations are coupled to distinct downstream effectors, which underlie the functions of many...
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09-09-2015 11:49 AM
[NMR paper] Uncovering the triggers for GPCR activation using solid-state NMR spectroscopy.
Uncovering the triggers for GPCR activation using solid-state NMR spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Uncovering the triggers for GPCR activation using solid-state NMR spectroscopy.
J Magn Reson. 2015 Apr;253:111-8
Authors: Kimata N, Reeves PJ, Smith SO
Abstract
G protein-coupled receptors (GPCRs) span cell membranes with seven transmembrane helices and respond to a diverse array of extracellular signals. Crystal structures of...
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03-24-2015 09:58 PM
[NMR paper] Uncovering the triggers for GPCR activation using solid-state NMR spectroscopy
Uncovering the triggers for GPCR activation using solid-state NMR spectroscopy
Publication date: April 2015
Source:Journal of Magnetic Resonance, Volume 253</br>
Author(s): Naoki Kimata , Philip J. Reeves , Steven O. Smith</br>
G protein-coupled receptors (GPCRs) span cell membranes with seven transmembrane helices and respond to a diverse array of extracellular signals. Crystal structures of GPCRs have provided key insights into the architecture of these receptors and the role of conserved residues. However, the question of how ligand binding induces the...
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03-22-2015 06:36 PM
[NMR paper] Conformational Ensembles in GPCR Activation.
Conformational Ensembles in GPCR Activation.
Related Articles Conformational Ensembles in GPCR Activation.
Cell. 2013 Jan 31;152(3):385-6
Authors: Vardy E, Roth BL
Abstract
Recent advances in G-protein-coupled receptor structural biology have provided only limited insight into the active conformations of these key signaling molecules. A paper from Nygaard et*al. reveals the dynamic nature of GPCRs along the activation pathway by complementing NMR experiments with ultralong-timescale molecular dynamics simulations.
Biphasic activation of beta-arrestin 1 upon interaction with a GPCR revealed by methyl-TROSY NMR
Linear Mode
