NMR paper A Bioorthogonal Click Chemistry Toolbox for Targeted Synthesis of Branched and Well-Defined Protein–Protein Conjugates

		BioNMR > Educational resources > Journal club
[NMR paper] A Bioorthogonal Click Chemistry Toolbox for Targeted Synthesis of Branched and Well-Defined Protein–Protein Conjugates

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

05-27-2020, 02:44 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

A Bioorthogonal Click Chemistry Toolbox for Targeted Synthesis of Branched and Well-Defined Protein–Protein Conjugates

A Bioorthogonal Click Chemistry Toolbox for Targeted Synthesis of Branched and Well-Defined Protein–Protein Conjugates

Angewandte Chemie International Edition, EarlyView.

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Liver-Targeted Small Molecule Inhibitors of Proprotein Convertase Subtilisin/Kexin Type 9 Synthesis Liver-Targeted Small Molecule Inhibitors of Proprotein Convertase Subtilisin/Kexin Type 9 Synthesis Targeting of the human ribosome is an unprecedented therapeutic modality with a genome-wide selectivity challenge. Herein we describe a liver-targeted drug candidate that inhibits ribosomal synthesis of PCSK9, a lipid regulator considered undruggable by small molecules. Key to the concept was the identification of pharmacologically active zwitterions designed to be retained in the liver. Oral delivery of the poorly permeable zwitterions was achieved via prodrugs susceptible to cleavage by...	nmrlearner	Journal club	0	10-27-2017 02:54 AM
[NMR paper] Characterization of conjugation pattern in large polysaccharide-protein conjugates by NMR. Characterization of conjugation pattern in large polysaccharide-protein conjugates by NMR. Related Articles Characterization of conjugation pattern in large polysaccharide-protein conjugates by NMR. Angew Chem Int Ed Engl. 2017 Oct 10;: Authors: Giuntini S, Balducci E, Cerofolini L, Ravera E, Fragai M, Berti F, Luchinat C Abstract Carbohydrate-based vaccines are among the safest and most effective vaccines and represent potent tools for prevention of life-threatening bacterial infectious diseases, like meningitis and pneumonia....	nmrlearner	Journal club	0	10-14-2017 02:04 AM
[NMR paper] Characterization of conjugation pattern in large polysaccharide-protein conjugates by NMR Characterization of conjugation pattern in large polysaccharide-protein conjugates by NMR Carbohydrate-based vaccines are among the safest and most effective vaccines and represent potent tools for prevention of life-threatening bacterial infectious diseases, like meningitis and pneumonia. The chemical conjugation of a weak antigen to protein as a source of T-cell epitopes generates a glycoconjugate vaccine, that results more immunogenic. Several methods have been used so far to characterize the resulting polysaccharide-protein conjugates. However, a reduced number of methodologies has...	nmrlearner	Journal club	0	10-10-2017 09:37 PM
[NMR paper] Orthogonal spin labeling using click chemistry for in vitro and in vivo applications Orthogonal spin labeling using click chemistry for in vitro and in vivo applications Publication date: Available online 2 December 2016 Source:Journal of Magnetic Resonance</br> Author(s): Svetlana Kucher, Sergei Korneev, Swati Tyagi, Ronja Apfelbaum, Dina Grohmann, Edward A. Lemke, Johann P. Klare, Heinz-Jürgen Steinhoff, Daniel Klose</br> Site-directed spin labeling for EPR- and NMR spectroscopy has mainly been achieved exploiting the specific reactivity of cysteines. For proteins with native cysteines or for in vivo applications, an alternative...	nmrlearner	Journal club	0	12-03-2016 02:09 PM
[NMR paper] Protein-Observed Fluorine NMR: A Bioorthogonal Approach for Small Molecule Discovery. Protein-Observed Fluorine NMR: A Bioorthogonal Approach for Small Molecule Discovery. Protein-Observed Fluorine NMR: A Bioorthogonal Approach for Small Molecule Discovery. J Med Chem. 2015 Nov 24; Authors: Arntson KE, Pomerantz WC Abstract The 19F isotope is 100% naturally abundant and is the second most sensitive and stable NMR-active nucleus. Unlike the ubiquitous hydrogen atom, fluorine is nearly absent in biological systems, making it a unique bioorthogonal atom for probing molecular interactions in biology. Over 73...	nmrlearner	Journal club	0	11-26-2015 12:13 AM
Diels-Alder reactionâ??triggered bioorthogonal protein decaging in living cells - Nature.com Diels-Alder reactionâ??triggered bioorthogonal protein decaging in living cells - Nature.com <img alt="" height="1" width="1" /> Diels-Alder reactionâ??triggered bioorthogonal protein decaging in living cells Nature.com A generally applicable strategy, however, remains elusive. Herein we describe a small moleculeâ??triggered bioorthogonal protein decaging technique that relies on the inverse electron-demand Diels-Alder reaction for eliminating a chemically caged protein ... Read here	nmrlearner	Online News	0	11-04-2014 02:32 PM
Â¿Â¿PT: a comprehensive toolbox for the analysis of protein motion - 7thSpace Interactive (press release) Â¿Â¿PT: a comprehensive toolbox for the analysis of protein motion - 7thSpace Interactive (press release) <img alt="" height="1" width="1" /> Â¿Â¿PT: a comprehensive toolbox for the analysis of protein motion 7thSpace Interactive (press release) Normal Mode Analysis is one of the most successful techniques for studying motions in proteins and macromolecules. It can provide information on the mechanism of protein functions, used to aid crystallography and NMR data reconstruction, and calculate ... Read here	nmrlearner	Online News	0	06-07-2013 10:42 PM
[NMR paper] Characterization of covalent protein conjugates using solid-state 13C NMR spectroscop Characterization of covalent protein conjugates using solid-state 13C NMR spectroscopy. Related Articles Characterization of covalent protein conjugates using solid-state 13C NMR spectroscopy. Biochemistry. 1991 Jul 23;30(29):7057-62 Authors: Garbow JR, Fujiwara H, Sharp CR, Logusch EW Cross-polarization magic-angle spinning (CPMAS) 13C NMR spectroscopy has been used to characterize covalent conjugates of alachlor, an alpha-chloroacetamide hapten, with glutathione (GSH) and bovine serum albumin (BSA). The solid-state NMR method demonstrates...	nmrlearner	Journal club	0	08-21-2010 11:12 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off