Recent advances in bioorthogonal catalysis promise to deliver new chemical tools for performing chemoselective transformations in complex biological environments. Herein we report how FAD (flavin adenine dinucleotide), FMN (flavin mononucleotide) and four flavoproteins behave as unconventional photocatalysts capable of converting PtIV and RuII complexes into potentially toxic PtII or RuII-OH2 species. Using electron donors and low doses of visible light, the flavoproteins mini Singlet Oxygen Generator (miniSOG) and NADH oxidase (NOX) catalytically activate PtIV prodrugs with bioorthogonal selectivity. In the presence of NADH, NOX catalyzes PtIV activation in the dark as well, indicating for the first time that flavoenzymes may contribute to initiate the activity of PtIV chemotherapeutic agents.
[NMR paper] Protein-Observed Fluorine NMR: A Bioorthogonal Approach for Small Molecule Discovery.
Protein-Observed Fluorine NMR: A Bioorthogonal Approach for Small Molecule Discovery.
Protein-Observed Fluorine NMR: A Bioorthogonal Approach for Small Molecule Discovery.
J Med Chem. 2015 Nov 24;
Authors: Arntson KE, Pomerantz WC
Abstract
The 19F isotope is 100% naturally abundant and is the second most sensitive and stable NMR-active nucleus. Unlike the ubiquitous hydrogen atom, fluorine is nearly absent in biological systems, making it a unique bioorthogonal atom for probing molecular interactions in biology. Over 73...
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11-26-2015 12:13 AM
TheQuest for Anticancer Vaccines: Deciphering theFine-Epitope Specificity of Cancer-Related Monoclonal Antibodies byCombining Microarray Screening and Saturation Transfer DifferenceNMR
TheQuest for Anticancer Vaccines: Deciphering theFine-Epitope Specificity of Cancer-Related Monoclonal Antibodies byCombining Microarray Screening and Saturation Transfer DifferenceNMR
Helena Coelho, Takahiko Matsushita, Gerard Artigas, Hiroshi Hinou, F. Javier Can?ada, Richard Lo-Man, Claude Leclerc, Eurico J. Cabrita, Jesu?s Jime?nez-Barbero, Shin-Ichiro Nishimura, Fayna Garcia-Marti?n and Filipa Marcelo
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.5b06787/20150922/images/medium/ja-2015-06787g_0004.gif
Journal of the...
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09-23-2015 11:52 AM
[NMR paper] Real-Time Monitoring of Cancer Cell Metabolism and Effects of an Anticancer Agent using 2D In-Cell NMR Spectroscopy.
Real-Time Monitoring of Cancer Cell Metabolism and Effects of an Anticancer Agent using 2D In-Cell NMR Spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary_FullTextOnline_120x27.gif Related Articles Real-Time Monitoring of Cancer Cell Metabolism and Effects of an Anticancer Agent using 2D In-Cell NMR Spectroscopy.
Angew Chem Int Ed Engl. 2015 Mar 5;
Authors: Wen H, An YJ, Xu WJ, Kang KW, Park S
Abstract
Altered metabolism is a critical part of...
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03-11-2015 09:59 PM
Diels-Alder reactionâ??triggered bioorthogonal protein decaging in living cells - Nature.com
Diels-Alder reactionâ??triggered bioorthogonal protein decaging in living cells - Nature.com
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Diels-Alder reactionâ??triggered bioorthogonal protein decaging in living cells
Nature.com
A generally applicable strategy, however, remains elusive. Herein we describe a small moleculeâ??triggered bioorthogonal protein decaging technique that relies on the inverse electron-demand Diels-Alder reaction for eliminating a chemically caged protein ...
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11-04-2014 02:32 PM
[NMR paper] Solid-state NMR of flavins and flavoproteins.
Solid-state NMR of flavins and flavoproteins.
Related Articles Solid-state NMR of flavins and flavoproteins.
Methods Mol Biol. 2014;1146:307-40
Authors: Miller AF
Abstract
Why apply solid-state NMR (SSNMR) to flavins and flavoproteins? NMR provides information on an atom-specific basis about chemical functionality, structure, proximity to other groups, and dynamics of the system. Thus, it has become indispensable to the study of chemicals, materials, catalysts, and biomolecules. It is no surprise then that NMR has a great deal to...
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04-26-2014 05:46 PM
[NMR paper] Insights into the Reactivity of Gold-Dithiocarbamato Anticancer Agents toward Model Biomolecules by Using Multinuclear NMR Spectroscopy.
Insights into the Reactivity of Gold-Dithiocarbamato Anticancer Agents toward Model Biomolecules by Using Multinuclear NMR Spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary-Button_120x27px_FullText.gif Related Articles Insights into the Reactivity of Gold-Dithiocarbamato Anticancer Agents toward Model Biomolecules by Using Multinuclear NMR Spectroscopy.
Chemistry. 2013 Sep 3;
Authors: Boscutti G, Marchiò L, Ronconi L, Fregona D
Abstract
Some...
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09-17-2013 11:36 PM
Mouthwash: An anticancer solution
Mouthwash: An anticancer solution
http://www.spectroscopynow.com/common/images/thumbnails/13da7b35fbf.jpgThe active ingredients in some commercially available mouthwash solution, the bisbiguanide oral disinfectants chlorhexidine (Chlorhexamed, Chlorhexal, Periogard, Corsodyl, and Chlorohex) and alexidine (Esemdent) have been in widespread use for many years. Now, 1H,15N-HSQC NMR spectroscopy has been used in studies to investigate the structural changes these compounds can induce that lead to apoptosis in oral cancer cells.
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04-01-2013 03:38 AM
[NMR paper] 19F NMR studies on 8-fluoroflavins and 8-fluoro flavoproteins.
19F NMR studies on 8-fluoroflavins and 8-fluoro flavoproteins.
Related Articles 19F NMR studies on 8-fluoroflavins and 8-fluoro flavoproteins.
Biochemistry. 1990 Mar 20;29(11):2670-9
Authors: Macheroux P, Kojiro CL, Schopfer LM, Chakraborty S, Massey V
The 19F NMR spectra of the oxidized and reduced forms of 8-fluororiboflavin, 8-fluoro-FAD, and the 8-fluoroflavin-reconstituted flavoproteins flavodoxin, riboflavin binding protein, D-amino acid oxidase, p-hydroxybenzoate hydroxylase, Old Yellow Enzyme, anthranilate hydroxylase, general acyl-CoA...
Bioorthogonal Catalytic Activation Of Pt And Ru Anticancer Complexes By FAD And Flavoproteins
Linear Mode
