NMR paper Biomolecular magic-angle spinning solid-state NMR: recent methods and applications.

		BioNMR > Educational resources > Journal club
[NMR paper] Biomolecular magic-angle spinning solid-state NMR: recent methods and applications.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

03-14-2013, 10:05 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Biomolecular magic-angle spinning solid-state NMR: recent methods and applications.

Biomolecular magic-angle spinning solid-state NMR: recent methods and applications.

Related Articles Biomolecular magic-angle spinning solid-state NMR: recent methods and applications.

Curr Opin Biotechnol. 2013 Mar 4;

Authors: Goldbourt A

Abstract
The link of structure and dynamics of biomolecules and their complexes to their function and to many cellular processes has driven the quest for their detailed characterization by a variety of biophysical techniques. Magic-angle spinning solid-state nuclear magnetic resonance spectroscopy provides detailed information on the structural properties of such systems and in particular contributes invaluable information on non-soluble, large molecular-weight and non-crystalline biomolecules. This review summarizes the recent progress that has been made in the characterization of macromolecular assemblies, viruses, membrane proteins, amyloid fibrils, protein aggregates and more by magic-angle spinning solid-state NMR.

PMID: 23481376 [PubMed - as supplied by publisher]

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
3D DUMAS: Simultaneous acquisition of three-dimensional magic angle spinning solid-state NMR experiments of proteins 3D DUMAS: Simultaneous acquisition of three-dimensional magic angle spinning solid-state NMR experiments of proteins July 2012 Publication year: 2012 Source:Journal of Magnetic Resonance, Volume 220</br> </br> Using the DUMAS (Dual acquisition Magic Angle Spinning) solid-state NMR approach, we created new pulse schemes that enable the simultaneous acquisition of three dimensional (3D) experiments on uniformly 13C, 15N labeled proteins. These new experiments exploit the simultaneous cross-polarization (SIM-CP) from 1H to 13C and 15N to acquire two 3D experiments...	nmrlearner	Journal club	0	02-03-2013 10:13 AM
Solid-state magic-angle spinning NMR of membrane proteins and protein–ligand interactions Solid-state magic-angle spinning NMR of membrane proteins and protein–ligand interactions April 2012 Publication year: 2012 Source:European Journal of Cell Biology, Volume 91, Issue 4</br> </br> Structural biology is developing into a universal tool for visualizing biological processes in space and time at atomic resolution. The field has been built by established methodology like X-ray crystallography, electron microscopy and solution NMR and is now incorporating new techniques, such as small-angle X-ray scattering, electron tomography, magic-angle-spinning solid-state...	nmrlearner	Journal club	0	02-03-2013 10:13 AM
3D DUMAS: Simultaneous Acquisition of Three-Dimensional Magic Angle Spinning Solid-State NMR Experiments of Proteins 3D DUMAS: Simultaneous Acquisition of Three-Dimensional Magic Angle Spinning Solid-State NMR Experiments of Proteins Publication year: 2012 Source:Journal of Magnetic Resonance</br> T. Gopinath, Gianluigi Veglia</br> Using the DUMAS (Dual acquisition Magic Angle Spinning) solid-state NMR approach, we created new pulse schemes that enable the simultaneous acquisition of three dimensional (3D) experiments on uniformly 13C, 15N labeled proteins. These new experiments exploit the simultaneous cross-polarization (SIM-CP) from 1H to 13C and 15N to acquire two 3D experiments...	nmrlearner	Journal club	0	04-26-2012 08:10 PM
[NMR paper] Solid-state magic-angle spinning NMR of outer-membrane protein G from Escherichia coli. Solid-state magic-angle spinning NMR of outer-membrane protein G from Escherichia coli. Related Articles Solid-state magic-angle spinning NMR of outer-membrane protein G from Escherichia coli. Chembiochem. 2005 Sep;6(9):1679-84 Authors: Hiller M, Krabben L, Vinothkumar KR, Castellani F, van Rossum BJ, Kühlbrandt W, Oschkinat H Uniformly 13C-,15N-labelled outer-membrane protein G (OmpG) from Escherichia coli was expressed for structural studies by solid-state magic-angle spinning (MAS) NMR. Inclusion bodies of the recombinant, labelled protein...	nmrlearner	Journal club	0	12-01-2010 06:56 PM
[NMR paper] Magic angle spinning solid-state NMR spectroscopy for structural studies of protein i Magic angle spinning solid-state NMR spectroscopy for structural studies of protein interfaces. resonance assignments of differentially enriched Escherichia coli thioredoxin reassembled by fragment complementation. Related Articles Magic angle spinning solid-state NMR spectroscopy for structural studies of protein interfaces. resonance assignments of differentially enriched Escherichia coli thioredoxin reassembled by fragment complementation. J Am Chem Soc. 2004 Dec 22;126(50):16608-20 Authors: Marulanda D, Tasayco ML, McDermott A, Cataldi M, Arriaran V,...	nmrlearner	Journal club	0	11-24-2010 10:03 PM
[NMR paper] Structure of a protein determined by solid-state magic-angle-spinning NMR spectroscop Structure of a protein determined by solid-state magic-angle-spinning NMR spectroscopy. Related Articles Structure of a protein determined by solid-state magic-angle-spinning NMR spectroscopy. Nature. 2002 Nov 7;420(6911):98-102 Authors: Castellani F, van Rossum B, Diehl A, Schubert M, Rehbein K, Oschkinat H The determination of a representative set of protein structures is a chief aim in structural genomics. Solid-state NMR may have a crucial role in structural investigations of those proteins that do not easily form crystals or are not...	nmrlearner	Journal club	0	11-24-2010 08:58 PM
Automated protein resonance assignments of magic angle spinning solid-state NMR spect Automated protein resonance assignments of magic angle spinning solid-state NMR spectra of Î²1 immunoglobulin binding domain of protein G (GB1) Abstract Magic-angle spinning solid-state NMR (MAS SSNMR) represents a fast developing experimental technique with great potential to provide structural and dynamics information for proteins not amenable to other methods. However, few automated analysis tools are currently available for MAS SSNMR. We present a methodology for automating protein resonance assignments of MAS SSNMR spectral data and its application to experimental peak lists of the...	nmrlearner	Journal club	0	10-15-2010 05:16 PM
Automated protein resonance assignments of magic angle spinning solid-state NMR spect Automated protein resonance assignments of magic angle spinning solid-state NMR spectra of ?1 immunoglobulin binding domain of protein G (GB1). Related Articles Automated protein resonance assignments of magic angle spinning solid-state NMR spectra of ?1 immunoglobulin binding domain of protein G (GB1). J Biomol NMR. 2010 Oct 8; Authors: Moseley HN, Sperling LJ, Rienstra CM Magic-angle spinning solid-state NMR (MAS SSNMR) represents a fast developing experimental technique with great potential to provide structural and dynamics information for...	nmrlearner	Journal club	0	10-12-2010 02:52 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off