NMR paper Biomimicry of artificial spider silk spinning assessed by NMR

		BioNMR > Educational resources > Journal club
[NMR paper] Biomimicry of artificial spider silk spinning assessed by NMR

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-09-2017, 09:26 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Biomimicry of artificial spider silk spinning assessed by NMR

Biomimicry of artificial spider silk spinning assessed by NMR

Biomimetic spinning of artificial spider silk requires that the terminal domains of designed minispidroins undergo specific structural changes in concert with the ?-sheet conversion of the repetitive region. Here, we combine solution and solid state NMR methods to probe domain-specific structural changes in the NT2RepCT minispidroin, which allows us to assess the degree of biomimicry of artificial silk spinning. In addition, we show that the structural effects of post-spinning procedures can be examined. By studying the impact of NT2RepCT fiber drying we observed a reversible beta-to-alpha conversion. We think that this approach will be useful for guiding the optimization of artificial spider silk fibers.

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Tracking Transitions in Spider Wrapping Silk Conformation and Dynamics by (19)F-NMR Spectroscopy. Tracking Transitions in Spider Wrapping Silk Conformation and Dynamics by (19)F-NMR Spectroscopy. Tracking Transitions in Spider Wrapping Silk Conformation and Dynamics by (19)F-NMR Spectroscopy. Biochemistry. 2016 May 6; Authors: Sarker M, Orrell KE, Xu L, Tremblay ML, Bak JJ, Liu XQ, Rainey JK Abstract Aciniform silk protein (AcSp1) is the primary component of wrapping silk, the toughest of the spider silks due to combined high tensile strength and extensibility. Argiope trifasciata AcSp1 contains a core repetitive domain with...	nmrlearner	Journal club	0	05-07-2016 06:32 PM
[NMR paper] Elucidating proline dynamics in spider dragline silk fibre using 2H-13C HETCOR MAS NMR. Elucidating proline dynamics in spider dragline silk fibre using 2H-13C HETCOR MAS NMR. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.rsc.org-images-entities-char_z_RSClogo.gif Related Articles Elucidating proline dynamics in spider dragline silk fibre using 2H-13C HETCOR MAS NMR. Chem Commun (Camb). 2014 May 14;50(37):4856-9 Authors: Shi X, Yarger JL, Holland GP Abstract (2)H-(13)C HETCOR MAS NMR is performed on (2)H/(13)C/(15)N-Pro enriched A. aurantia dragline silk. Proline dynamics are extracted...	nmrlearner	Journal club	0	04-22-2015 03:33 PM
Arachnid Rapunzel: Researchers spin spider silk proteins into artificial silk - (e) Science News Arachnid Rapunzel: Researchers spin spider silk proteins into artificial silk - (e) Science News http://www.bionmr.com//t0.gstatic.com/images?q=tbn:ANd9GcRr_N95y_3k4uVB81DfIFgrFibP4sVEYf3t9tZgG9j9dVXLKH5CbficPDLAVrQhoOLzuZFxsKhI (e) Science News <img alt="" height="1" width="1"> Arachnid Rapunzel: Researchers spin spider silk proteins into artificial silk (e) Science News Jan Rainey's group at Dalhousie University used nuclear magnetic resonance (NMR) spectroscopy to analyze the structure of AcSp1's repeat sequence at very high resolution, producing one of the first spider silk repeat...	nmrlearner	Online News	0	02-12-2015 06:10 AM
Arachnid Rapunzel: Researchers spin spider silk proteins into artificial silk - Nanowerk Arachnid Rapunzel: Researchers spin spider silk proteins into artificial silk - Nanowerk http://www.bionmr.com//t1.gstatic.com/images?q=tbn:ANd9GcQOWTtIYCuZDjceklf47iupyefX8WwW65a7h21uZE7UoWLopYGYitzsPaI0uLriPC5sDeCwkRY Nanowerk <img alt="" height="1" width="1"> Arachnid Rapunzel: Researchers spin spider silk proteins into artificial silk Nanowerk Jan Rainey's group at Dalhousie University used nuclear magnetic resonance (NMR) spectroscopy to analyze the structure of AcSp1's repeat sequence at very high resolution, producing one of the first spider silk repeat unit structure sequences...	nmrlearner	Online News	0	02-11-2015 12:26 AM
[NMR paper] Molecular Dynamics of Spider Dragline Silk Fiber Investigated by 2H MAS NMR. Molecular Dynamics of Spider Dragline Silk Fiber Investigated by 2H MAS NMR. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Molecular Dynamics of Spider Dragline Silk Fiber Investigated by 2H MAS NMR. Biomacromolecules. 2015 Jan 25; Authors: Shi X, Holland GP, Yarger JL Abstract	nmrlearner	Journal club	0	01-28-2015 03:22 AM
Exploring the Backbone Dynamics of Native Spider Silk Proteins in Black Widow Silk Glands with Solution-state NMR Spectroscopy Exploring the Backbone Dynamics of Native Spider Silk Proteins in Black Widow Silk Glands with Solution-state NMR Spectroscopy Publication date: Available online 13 June 2014 Source:Polymer</br> Author(s): Dian Xu , Jeffery L. Yarger , Gregory P. Holland</br> Spider dragline silk is an outstanding biopolymer with a strength that exceeds steel by weight and a toughness greater than high-performance fibers like Kevlar. For this reason, understanding how a spider converts the gel-like, aqueous protein spinning dope within the major ampullate (MA) gland into a super...	nmrlearner	Journal club	0	06-14-2014 12:53 AM
[NMR paper] Amino Acid Analysis of spider dragline silk using (1)H NMR. Amino Acid Analysis of spider dragline silk using (1)H NMR. Related Articles Amino Acid Analysis of spider dragline silk using (1)H NMR. Anal Biochem. 2013 May 30; Authors: Shi X, Holland GP, Yarger JL Abstract The amino acid composition of N. clavipes dragline silk fiber is determined by conducting (1)H Nuclear Magnetic Resonance (NMR) spectroscopy experiments on acid hydrolyzed material. N. clavipes dragline silk was found to consist of 43.0±0.6% Gly, 29.3±0.2% Ala, 9.1±0.1% Glx, 4.0±0.1% Leu, 3.3±0.1% Tyr, 3.4 ±0.2% Ser, 2.7±0.1%...	nmrlearner	Journal club	0	06-04-2013 06:31 PM
[NMR paper] NMR characterization of native liquid spider dragline silk from Nephila edulis. NMR characterization of native liquid spider dragline silk from Nephila edulis. Related Articles NMR characterization of native liquid spider dragline silk from Nephila edulis. Biomacromolecules. 2004 May-Jun;5(3):834-9 Authors: Hronska M, van Beek JD, Williamson PT, Vollrath F, Meier BH Solid spider dragline silk is well-known for its mechanical properties. Nonetheless a detailed picture of the spinning process is lacking. Here we report NMR studies on the liquid silk within the wide sac of the major ampullate (m.a.) gland from the spider...	nmrlearner	Journal club	0	11-24-2010 09:51 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off