We demonstrate an atom-efficient and easy to use H(2)-driven biocatalytic platform for the enantioselective incorporation of ˛H-atoms into amino acids. By combining the biocatalytic deuteration catalyst with amino acid dehydrogenase enzymes capable of reductive amination, we synthesised a library of multiply isotopically labelled amino acids from low-cost isotopic precursors, such as ˛H(2)O and ^(15)NH(4)^(+). The chosen approach avoids the use of pre-labeled ˛H-reducing agents, and therefore...
Improved spectral resolution of [13C,1H]-HSQC spectra of aromatic amino acid residues in proteins produced by cell-free synthesis from inexpensive 13C-labelled precursors
Improved spectral resolution of -HSQC spectra of aromatic amino acid residues in proteins produced by cell-free synthesis from inexpensive 13C-labelled precursors
Abstract
Cell-free protein synthesis using eCells allows production of amino acids from inexpensive 13C-labelled precursors. We show that the metabolic pathway converting pyruvate, glucose and erythrose into aromatic amino acids is maintained in eCells. Judicious choice of 13C-labelled starting material leads to proteins, where the sidechains of aromatic amino acids display -HSQC cross-peaks...
Revealing the role of the mysterious small proteins: Classifying proteins with less than 100 amino acids - Science Daily
Revealing the role of the mysterious small proteins: Classifying proteins with less than 100 amino acids - Science Daily
Revealing the role of the mysterious small proteins: Classifying proteins with less than 100 amino acids Science DailyInvestigators develop a technique to identify and classify proteins with less than 100 amino acids. These types of proteins account for only 16 percent of a ...
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[NMR paper] Synthesis of Isotopically Labelled All-Trans Retinals for DNP-Enhanced Solid State NMR Studies of Retinylidene Proteins.
Synthesis of Isotopically Labelled All-Trans Retinals for DNP-Enhanced Solid State NMR Studies of Retinylidene Proteins.
Related Articles Synthesis of Isotopically Labelled All-Trans Retinals for DNP-Enhanced Solid State NMR Studies of Retinylidene Proteins.
J Labelled Comp Radiopharm. 2017 Oct 28;:
Authors: Leeder AJ, Brown LJ, Becker-Baldus J, Mehler M, Glaubitz C, Brown RCD
Abstract
Three all-trans retinals containing multiple (13) C labels have been synthesised to enable DNP enhanced solid-state MAS NMR studies of novel...
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10-29-2017 02:06 PM
[NMR paper] Photo-CIDNP NMR Spectroscopy of Amino Acids and Proteins.
Photo-CIDNP NMR Spectroscopy of Amino Acids and Proteins.
Related Articles Photo-CIDNP NMR Spectroscopy of Amino Acids and Proteins.
Top Curr Chem. 2013 May 14;
Authors: Kuhn LT
Abstract
Photo-chemically induced dynamic nuclear polarization (CIDNP) is a nuclear magnetic resonance (NMR) phenomenon which, among other things, is exploited to extract information on biomolecular structure via probing solvent-accessibilities of tryptophan (Trp), tyrosine (Tyr), and histidine (His) amino acid side chains both in polypeptides and proteins in...
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[NMR paper] Assigning the NMR spectra of aromatic amino acids in proteins: analysis of two Ets po
Assigning the NMR spectra of aromatic amino acids in proteins: analysis of two Ets pointed domains.
Related Articles Assigning the NMR spectra of aromatic amino acids in proteins: analysis of two Ets pointed domains.
Biochem Cell Biol. 1998;76(2-3):379-90
Authors: Slupsky CM, Gentile LN, McIntosh LP
The measurement of interproton nuclear Overhauser enhancements (NOEs) and dihedral angle restraints of aromatic amino acids is a critical step towards determining the structure of a protein. The complete assignment of the resonances from aromatic...
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Production of recombinant isotopically labelled peptide by fusion to an insoluble par
Production of recombinant isotopically labelled peptide by fusion to an insoluble partner protein: generation of integrin ?v?6 binding peptides for NMR.
Related Articles Production of recombinant isotopically labelled peptide by fusion to an insoluble partner protein: generation of integrin ?v?6 binding peptides for NMR.
Mol Biosyst. 2010 Oct 18;
Authors: Wagstaff JL, Howard MJ, Williamson RA
The integrin ?v?6 is up-regulated in several cancers and has clinical potential for both tumour imaging and therapy. Peptide ligands have been developed...
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[NMR paper] Determination of de novo synthesized amino acids in cellular proteins revisited by 13
Determination of de novo synthesized amino acids in cellular proteins revisited by 13C NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_120x27.gif Related Articles Determination of de novo synthesized amino acids in cellular proteins revisited by 13C NMR spectroscopy.
NMR Biomed. 1997 Apr;10(2):50-8
Authors: Flögel U, Willker W, Leibfritz D
13C nuclear magnetic resonance spectroscopy was used to determine the absolute amounts to de novo...
Biocatalytic reductive amination as a route to isotopically labelled amino acids suitable for analysis of large proteins by NMR
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