[NMR paper] NMR and EPR reveal a compaction of the RNA-binding protein FUS upon droplet formation
NMR and EPR reveal a compaction of the RNA-binding protein FUS upon droplet formation
Many RNA-binding proteins undergo liquid-liquid phase separation, which underlies the formation of membraneless organelles, such as stress granules and P-bodies. Studies of the molecular mechanism of phase separation in vitro are hampered by the coalescence and sedimentation of organelle-sized droplets interacting with glass surfaces. Here, we demonstrate that liquid droplets of fused in sarcoma (FUS)-a protein found in cytoplasmic aggregates of amyotrophic lateral sclerosis and frontotemporal...
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03-09-2021 02:17 PM
[ASAP] CRISPR-Cas13-Based RNA-Interacting Protein Detection in Living Cells
CRISPR-Cas13-Based RNA-Interacting Protein Detection in Living Cells
https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.0c00281/20200429/images/medium/bi0c00281_0001.gif
Biochemistry
DOI: 10.1021/acs.biochem.0c00281
http://feeds.feedburner.com/~r/acs/bichaw/~4/NV3_snWqzIU
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04-30-2020 02:58 PM
Tiny Protein Has Huge Potential in CRISPR - Technology Networks
Tiny Protein Has Huge Potential in CRISPR - Technology Networks
Tiny Protein Has Huge Potential in CRISPR Technology NetworksAn array of CRISPR proteins now exist, each presenting with their own unique properties. Last year the smallest CRISPR protein, Cas14 was discovered.
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04-16-2019 06:39 PM
[NMR paper] Optical control of protein pattern formation
Optical control of protein pattern formation
Patterns formed by protein reactions and diffusion are the foundation for many phenomena in biology. Yet, the experimental study of reaction-diffusion (R-D) systems has so far been dominated by chemical oscillators, for which many manipulation tools are available. Here, we developed a photoswitch for the Min system of Escherichia coli, a versatile biological in vitro R-D system consisting of the antagonistic proteins MinD and MinE. A MinE-derived peptide of 19 amino acids is covalently modified with a photoisomerizable crosslinker based on...
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12-23-2017 06:08 PM
[NMR paper] Peptide-Directed Binding for the Discovery of Modulators of ?-Helix-Mediated Protein–Protein Interactions: Proof-of-Concept Studies with the Apoptosis Regulator Mcl-1
Peptide-Directed Binding for the Discovery of Modulators of ?-Helix-Mediated Protein–Protein Interactions: Proof-of-Concept Studies with the Apoptosis Regulator Mcl-1
Targeting PPIs with small molecules can be challenging owing to large, hydrophobic binding surfaces. Herein, we describe a strategy that exploits selective ?-helical PPIs, transferring these characteristics to small molecules. The proof of concept is demonstrated with the apoptosis regulator Mcl-1, commonly exploited by cancers to avoid cell death. Peptide-directed binding uses few synthetic transformations, requires the...
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07-25-2017 07:46 PM
[NMR paper] Peptide directed binding; a novel approach for the discovery of modulators of alpha-helix mediated protein-protein interactions demonstrated with apoptosis regulating Mcl-1
Peptide directed binding; a novel approach for the discovery of modulators of alpha-helix mediated protein-protein interactions demonstrated with apoptosis regulating Mcl-1
Targeting PPIs with small molecules can be challenging due to large, hydrophobic binding surfaces. Here, we describe a strategy that exploits selective alpha-helical PPIs, transferring these characteristics to small molecules. The proof-of-concept is exemplified with the apoptosis regulator Mcl-1, commonly exploited by cancers to avoid cell death. Peptide directed binding uses few synthetic transformations, requires...
[NMR paper] Solution properties of the archaeal CRISPR DNA repeat-binding homeodomain protein Cbp2.
Solution properties of the archaeal CRISPR DNA repeat-binding homeodomain protein Cbp2.
Related Articles Solution properties of the archaeal CRISPR DNA repeat-binding homeodomain protein Cbp2.
Nucleic Acids Res. 2013 Jan 15;
Authors: Kenchappa CS, Heidarsson PO, Kragelund BB, Garrett RA, Poulsen FM
Abstract
Clustered regularly interspaced short palindromic repeats (CRISPR) form the basis of diverse adaptive immune systems directed primarily against invading genetic elements of archaea and bacteria. Cbp1 of the crenarchaeal...
Binding-Mediated Formation of Ribonucleoprotein Corona for Efficient Delivery and Control of CRISPR/Cas9
Linear Mode
