We have used NMR experiments to explore binding of selected glycans and glycomimetics to the SARS CoV-2 spike glycoprotein (S-protein) and to its receptor binding domain (RBD). STD NMR experiments confirm binding of sialoglycans to the S-protein of the prototypic Wuhan strain virus and yield dissociation constants in the mM range. The absence of STD effects for sialoglycans in the presence of the Omicron/BA.1 S-protein reflects a loss of binding as a result of S-protein evolution. Likewise, no...
[ASAP] NMR Experiments Provide Insights into Ligand-Binding to the SARS-CoV-2 Spike Protein Receptor-Binding Domain
NMR Experiments Provide Insights into Ligand-Binding to the SARS-CoV-2 Spike Protein Receptor-Binding Domain
Robert Creutznacher, Thorben Maass, Barbora Veselkova, George Ssebyatika, Thomas Krey, Martin Empting, Norbert Tautz, Martin Frank, Knut Ko?lbel, Charlotte Uetrecht, and Thomas Peters
https://pubs.acs.org/cms/10.1021/jacs.2c05603/asset/images/medium/ja2c05603_0004.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.2c05603
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[NMR paper] NMR Experiments Provide Insights into Ligand-Binding to the SARS-CoV-2 Spike Protein Receptor-Binding Domain
NMR Experiments Provide Insights into Ligand-Binding to the SARS-CoV-2 Spike Protein Receptor-Binding Domain
We have used chemical shift perturbation (CSP) and saturation transfer difference (STD) NMR experiments to identify and characterize the binding of selected ligands to the receptor-binding domain (RBD) of the spike glycoprotein (S-protein) of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We also subjected full-length S-protein to STD NMR experiments, allowing correlations with RBD-based results. CSPs reveal the binding sites for heparin and fondaparinux, and...
[NMR paper] Structural characterization of the N-linked glycans in the receptor binding domain of the SARS-CoV-2 spike protein and*their interactions with human lectins using NMR spectroscopy.
Structural characterization of the N-linked glycans in the receptor binding domain of the SARS-CoV-2 spike protein and*their interactions with human lectins using NMR spectroscopy.
Structural characterization of the N-linked glycans in the receptor binding domain of the SARS-CoV-2 spike protein and*their interactions with human lectins using NMR spectroscopy.
Angew Chem Int Ed Engl. 2020 Sep 11;:
Authors: Lenza MP, Oyenarte I, Diercks T, Quintana JI, Gimeno A, Bosch A, Coelho H, Diniz A, Peccati F, Delgado S, Valle M, Millet O, Abrescia NGA,...
Binding of Glycans to the SARS CoV-2 Spike Protein, an Open Question: NMR Data on Binding Site Localization, Affinity, and Selectivity
Linear Mode
