[NMR paper] Behavioral, neurochemical and morphological changes induced by the overexpression of munc18-1a in brain of mice: relevance to schizophrenia.
Related ArticlesBehavioral, neurochemical and morphological changes induced by the overexpression of munc18-1a in brain of mice: relevance to schizophrenia.
Transl Psychiatry. 2013;3:e221
Authors: Urigüen L, Gil-Pisa I, Munarriz-Cuezva E, Berrocoso E, Pascau J, Soto-Montenegro ML, Gutiérrez-Adán A, Pintado B, Madrigal JL, Castro E, Sánchez-Blázquez P, Ortega JE, Guerrero MJ, Ferrer-Alcon M, García-Sevilla JA, Micó JA, Desco M, Leza JC, Pazos A, Garzón J, Meana JJ
Abstract
Overexpression of the mammalian homolog of the unc-18 gene (munc18-1) has been described in the brain of subjects with schizophrenia. Munc18-1 protein is involved in membrane fusion processes, exocytosis and neurotransmitter release. A transgenic mouse strain that overexpresses the protein isoform munc18-1a in the brain was characterized. This animal displays several schizophrenia-related behaviors, supersensitivity to hallucinogenic drugs and deficits in prepulse inhibition that reverse after antipsychotic treatment. Relevant brain areas (that is, cortex and striatum) exhibit reduced expression of dopamine D(1) receptors and dopamine transporters together with enhanced amphetamine-induced in vivo dopamine release. Magnetic resonance imaging demonstrates decreased gray matter volume in the transgenic animal. In conclusion, the mouse overexpressing brain munc18-1a represents a new valid animal model that resembles functional and structural abnormalities in patients with schizophrenia. The animal could provide valuable insights into phenotypic aspects of this psychiatric disorder.
Metabolic profiling of vitamin C deficiency in Guloâ??/â?? mice using proton NMR spectroscopy
Metabolic profiling of vitamin C deficiency in Guloâ??/â?? mice using proton NMR spectroscopy
Abstract Nutrient deficiencies are an ongoing problem in many populations and ascorbic acid is a key vitamin whose mild or acute absence leads to a number of conditions including the famously debilitating scurvy. As such, the biochemical effects of ascorbate deficiency merit ongoing scrutiny, and the Gulo knockout mouse provides a useful model for the metabolomic examination of vitamin C deficiency. Like humans, these animals are incapable of synthesizing ascorbic acid but with dietary...
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[NMR paper] Overexpression, purification, and characterization of recombinant Ca-ATPase regulator
Overexpression, purification, and characterization of recombinant Ca-ATPase regulators for high-resolution solution and solid-state NMR studies.
Related Articles Overexpression, purification, and characterization of recombinant Ca-ATPase regulators for high-resolution solution and solid-state NMR studies.
Protein Expr Purif. 2003 Aug;30(2):253-61
Authors: Buck B, Zamoon J, Kirby TL, DeSilva TM, Karim C, Thomas D, Veglia G
Phospholamban (PLB) and Sarcolipin (SLN) are integral membrane proteins that regulate muscle contractility via direct...
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[NMR paper] Overexpression and purification of isotopically labeled Escherichia coli MutH for NMR
Overexpression and purification of isotopically labeled Escherichia coli MutH for NMR studies.
Related Articles Overexpression and purification of isotopically labeled Escherichia coli MutH for NMR studies.
Protein Expr Purif. 2003 Jun;29(2):252-8
Authors: Dutta A, Rao BJ, Chary KV
MutH is one of the enzymes involved in the methyl directed -GATC-based DNA repair system. We report a significantly optimized protocol to prepare isotopically (15N and/or 13C) labeled MutH in minimal medium with high yields for NMR studies. Under the various...
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[NMR paper] NMR investigations of protein-carbohydrate interactions: studies on the relevance of
NMR investigations of protein-carbohydrate interactions: studies on the relevance of Trp/Tyr variations in lectin binding sites as deduced from titration microcalorimetry and NMR studies on hevein domains. Determination of the NMR structure of the complex between pseudohevein and N,N',N"-triacetylchitotriose.
Related Articles NMR investigations of protein-carbohydrate interactions: studies on the relevance of Trp/Tyr variations in lectin binding sites as deduced from titration microcalorimetry and NMR studies on hevein domains. Determination of the NMR structure of the complex...
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[NMR paper] Bacterial overexpression, isotope enrichment, and NMR analysis of the N-terminal doma
Bacterial overexpression, isotope enrichment, and NMR analysis of the N-terminal domain of human apolipoprotein E.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.nrc-cnrc.gc.ca-cisti-journals-rp-gifs-PubMed_logo_e.gif Related Articles Bacterial overexpression, isotope enrichment, and NMR analysis of the N-terminal domain of human apolipoprotein E.
Biochem Cell Biol. 1997;75(1):45-53
Authors: Fisher CA, Wang J, Francis GA, Sykes BD, Kay CM, Ryan RO
The nucleotide sequence encoding the N-terminal domain (residues 1-183) of human...
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[NMR paper] Overexpression of Desulfovibrio vulgaris Hildenborough cytochrome c553 in Desulfovibr
Overexpression of Desulfovibrio vulgaris Hildenborough cytochrome c553 in Desulfovibrio desulfuricans G200. Evidence of conformational heterogeneity in the oxidized protein by NMR.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Overexpression of Desulfovibrio vulgaris Hildenborough cytochrome c553 in Desulfovibrio desulfuricans G200. Evidence of conformational heterogeneity in the oxidized protein by NMR.
Eur J Biochem. 1993 Dec 1;218(2):293-301
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[NMR paper] NMR detection of creatine kinase expressed in liver of transgenic mice: determination
NMR detection of creatine kinase expressed in liver of transgenic mice: determination of free ADP levels.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles NMR detection of creatine kinase expressed in liver of transgenic mice: determination of free ADP levels.
Proc Natl Acad Sci U S A. 1990 Apr;87(8):3112-6
Authors: Koretsky AP, Brosnan MJ, Chen LH, Chen JD, Van Dyke T
To use the equilibrium established by creatine kinase (CK) to determine hepatic free ADP...
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Trends in solid-state NMR spectroscopy and their relevance for bioanalytics.
Trends in solid-state NMR spectroscopy and their relevance for bioanalytics.
Related Articles Trends in solid-state NMR spectroscopy and their relevance for bioanalytics.
Anal Bioanal Chem. 2010 Aug 14;
Authors: Paasch S, Brunner E
Based on continuous methodical advances and developments, solid-state NMR spectroscopy has become a powerful tool for the investigation of various materials, including polymers, glasses, zeolites, fullerenes, and many others. During the past decade, solid-state NMR spectroscopy also found increasing interest for the...
Behavioral, neurochemical and morphological changes induced by the overexpression of munc18-1a in brain of mice: relevance to schizophrenia.
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