Related ArticlesBead-linked proteoliposomes: a reconstitution method for nmr analyses of membrane protein-ligand interactions.
J Am Chem Soc. 2005 Aug 31;127(34):12021-7
Authors: Yokogawa M, Takeuchi K, Shimada I
Structural information about the interactions between membrane proteins and their ligands provides insights into the membrane protein functions. A variety of surfactants have been used for structural analyses of membrane proteins, and in some cases, they yielded successful results. However, the use of surfactants frequently increases the conformational instability of membrane proteins and distorts their normal function. Here, we propose a new strategy of membrane protein reconstitution into lipid bilayers on affinity beads, which maintains the native conformation and function of the protein for NMR studies. The reconstituted membrane proteins are suitable for NMR analyses of interactions, by using the transferred cross-saturation method. The strategy was successfully applied to the interaction between a potassium ion channel, KcsA, and a pore-blocker, agitoxin2 (AgTx2). This strategy would be useful for analyzing the interactions between various membrane proteins and their ligands.
[Question from NMRWiki Q&A forum] protein-ligand interactions 2D NMR
protein-ligand interactions 2D NMR
I want to judge ligand protein interactions. Mine one is a dimer protein. Other than HSQC perturbation which other 2DNMR experiments useful to know the interaction?
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05-18-2011 08:51 PM
Bacterial expression, purification, and model membrane reconstitution of the transmembrane and cytoplasmic domains of the human APP binding protein LR11/SorLA for NMR studies.
Bacterial expression, purification, and model membrane reconstitution of the transmembrane and cytoplasmic domains of the human APP binding protein LR11/SorLA for NMR studies.
Bacterial expression, purification, and model membrane reconstitution of the transmembrane and cytoplasmic domains of the human APP binding protein LR11/SorLA for NMR studies.
Protein Expr Purif. 2011 Feb 11;
Authors: Wang X, Gill Jr RL, Zhu Q, Tian F
LR11 (SorLA) is a recently identified neuronal protein that interacts with amyloid precursor protein (APP), a central player...
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02-16-2011 07:40 PM
[NMR paper] Studies of protein-ligand interactions by NMR.
Studies of protein-ligand interactions by NMR.
Related Articles Studies of protein-ligand interactions by NMR.
Biochem Soc Trans. 2003 Oct;31(Pt 5):1006-9
Authors: Clarkson J, Campbell ID
Solution-state NMR has become an accepted method for studying the structure of small proteins in solution. This has resulted in over 3000 NMR-based co-ordinate sets being deposited in the Protein Databank. It is becoming increasingly apparent, however, that NMR is also a very powerful tool for accessing interactions between macromolecules and various ligands....
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11-24-2010 09:16 PM
[NMR paper] NMR analyses of the interactions of human annexin I with ATP, Ca2+, and Mg2+.
NMR analyses of the interactions of human annexin I with ATP, Ca2+, and Mg2+.
Related Articles NMR analyses of the interactions of human annexin I with ATP, Ca2+, and Mg2+.
FEBS Lett. 1998 Apr 3;425(3):523-7
Authors: Han HY, Lee YH, Oh JY, Na DS, Lee BJ
Human annexin I is a member of the annexin family of calcium-dependent phospholipid binding proteins. The structure of an N-terminally truncated human annexin I (delta-annexin I) and its interactions with Ca2+, Mg2+, and ATP were studied at the atomic level using nuclear magnetic resonance...
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11-17-2010 11:06 PM
The STINT-NMR Method for Studying In-cell Protein-Protein Interactions.
The STINT-NMR Method for Studying In-cell Protein-Protein Interactions.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_120x27.gif Related Articles The STINT-NMR Method for Studying In-cell Protein-Protein Interactions.
Curr Protoc Protein Sci. 2010 Aug;Chapter 17:Unit17.11
Authors: Burz DS, Shekhtman A
This unit describes critical components and considerations required to study protein-protein structural interactions inside a living cell by using NMR...
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09-05-2010 05:53 AM
[NMR paper] Studies of protein-ligand interactions by NMR.
Studies of protein-ligand interactions by NMR.
Related Articles Studies of protein-ligand interactions by NMR.
Methods Mol Biol. 1997;60:195-232
Authors: Craik DJ, Wilce JA
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08-22-2010 03:31 PM
[NMR paper] Studies of protein-ligand interactions by NMR.
Studies of protein-ligand interactions by NMR.
Related Articles Studies of protein-ligand interactions by NMR.
Methods Mol Biol. 1997;60:195-232
Authors: Craik DJ, Wilce JA
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08-22-2010 03:03 PM
[NMR paper] On the ligand-protein and ligand-flavin interactions in NADPH-adrenodoxin reductase a
On the ligand-protein and ligand-flavin interactions in NADPH-adrenodoxin reductase as studied by 31P- and 13C-NMR. Use of 13C-enriched FAD as a probe.
Related Articles On the ligand-protein and ligand-flavin interactions in NADPH-adrenodoxin reductase as studied by 31P- and 13C-NMR. Use of 13C-enriched FAD as a probe.
J Biochem. 1991 Jan;109(1):144-9
Authors: Fujii S, Nonaka Y, Okamoto M, Miura R
The interaction between 2',5'-ADP and NADPH-adrenodoxin reductase from bovine adrenocortical mitochondria was examined by titrating the enzyme with...
Bead-linked proteoliposomes: a reconstitution method for nmr analyses of membrane protein-ligand interactions.
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