[NMR paper] Activation of adenosine A2A receptor by lipids from docosahexaenoic acid revealed by NMR.
Activation of adenosine A2A receptor by lipids from docosahexaenoic acid revealed by NMR.
Related Articles Activation of adenosine A2A receptor by lipids from docosahexaenoic acid revealed by NMR.
Sci Adv. 2020 Mar;6(12):eaay8544
Authors: Mizumura T, Kondo K, Kurita M, Kofuku Y, Natsume M, Imai S, Shiraishi Y, Ueda T, Shimada I
Abstract
The lipid composition of the plasma membrane is a key parameter in controlling signal transduction through G protein-coupled receptors (GPCRs). Adenosine A2A receptor (A2AAR) is located in the...
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03-25-2020 10:28 PM
[NMR paper] (19)F-NMR screening of unrelated antimicrobial peptides shows that membrane interactions are largely governed by lipids.
(19)F-NMR screening of unrelated antimicrobial peptides shows that membrane interactions are largely governed by lipids.
(19)F-NMR screening of unrelated antimicrobial peptides shows that membrane interactions are largely governed by lipids.
Biochim Biophys Acta. 2014 Mar 31;
Authors: Afonin S, Glaser RW, Sachse C, Salgado J, Wadhwani P, Ulrich AS
Abstract
Many amphiphilic antimicrobial peptides permeabilize bacterial membranes via successive steps of binding, re-alignment and/or oligomerization. Here, we have systematically...
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04-06-2014 02:01 AM
[NMR paper] Preparation of Multiple Site-Specific Mutant Proteins for NMR Studies by PCR-Directed Cell-Free Protein Synthesis.
Preparation of Multiple Site-Specific Mutant Proteins for NMR Studies by PCR-Directed Cell-Free Protein Synthesis.
Preparation of Multiple Site-Specific Mutant Proteins for NMR Studies by PCR-Directed Cell-Free Protein Synthesis.
Methods Mol Biol. 2014;1118:169-87
Authors: Ozawa K, Qi R
Abstract
Cell-free protein synthesis (CFPS) offers a fast and inexpensive approach to selectively label proteins with isotopes that can then be detected by nuclear magnetic resonance (NMR) spectroscopy directly in the translation mixture. We describe...
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01-08-2014 11:23 AM
[NMR paper] Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins.
Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins.
Related Articles Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins.
J Vis Exp. 2013;(82)
Authors: Roberson KJ, Brady PN, Sweeney MM, Macnaughtan MA
Abstract
Nuclear magnetic resonance (NMR) spectroscopy is a proven technique for protein structure and dynamic studies. To study proteins with NMR, stable magnetic isotopes are typically incorporated metabolically to...
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01-01-2014 03:05 PM
[NMR paper] Bioconjugation of proteins with a paramagnetic NMR and fluorescent tag.
Bioconjugation of proteins with a paramagnetic NMR and fluorescent tag.
Related Articles Bioconjugation of proteins with a paramagnetic NMR and fluorescent tag.
Chemistry. 2013 Dec 9;19(50):17141-9
Authors: Huang F, Pei YY, Zuo HH, Chen JL, Yang Y, Su XC
Abstract
Site-specific labeling of proteins with lanthanide ions offers great opportunities for investigating the structure, function, and dynamics of proteins by virtue of the unique properties of lanthanides. Lanthanide-tagged proteins can be studied by NMR, X-ray, fluorescence, and EPR...
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12-07-2013 01:00 PM
Site-specific free energy changes in proteins upon ligand binding by NMR: Ca(2+) -displacement by Ln(3+) in a Ca(2+) -binding protein from Entamoeba histolytica.
Site-specific free energy changes in proteins upon ligand binding by NMR: Ca(2+) -displacement by Ln(3+) in a Ca(2+) -binding protein from Entamoeba histolytica.
Site-specific free energy changes in proteins upon ligand binding by NMR: Ca(2+) -displacement by Ln(3+) in a Ca(2+) -binding protein from Entamoeba histolytica.
Chem Biol Drug Des. 2011 Jan 14;
Authors: Chandra K, Mustafi SM, Muthukumar S, Chary KV
The study of protein-ligand interaction has been of a great interest in contemporary structural biology. The understanding of the nature...
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01-18-2011 10:22 PM
[NMR paper] Resolution of individual lipids in mixed phospholipid membranes and specific lipid-cy
Resolution of individual lipids in mixed phospholipid membranes and specific lipid-cytochrome c interactions by magic-angle spinning solid-state phosphorus-31 NMR.
Related Articles Resolution of individual lipids in mixed phospholipid membranes and specific lipid-cytochrome c interactions by magic-angle spinning solid-state phosphorus-31 NMR.
Biochemistry. 1994 Mar 8;33(9):2459-67
Authors: Pinheiro TJ, Watts A
A model of the inner mitochondrial membrane was constructed with dioleoyphosphatidylcholine (PC), dioleoylphosphatidylethanolamine...
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08-22-2010 03:33 AM
[NMR paper] Resolution of individual lipids in mixed phospholipid membranes and specific lipid-cy
Resolution of individual lipids in mixed phospholipid membranes and specific lipid-cytochrome c interactions by magic-angle spinning solid-state phosphorus-31 NMR.
Related Articles Resolution of individual lipids in mixed phospholipid membranes and specific lipid-cytochrome c interactions by magic-angle spinning solid-state phosphorus-31 NMR.
Biochemistry. 1994 Mar 8;33(9):2459-67
Authors: Pinheiro TJ, Watts A
A model of the inner mitochondrial membrane was constructed with dioleoyphosphatidylcholine (PC), dioleoylphosphatidylethanolamine...
Azaphilones as Activation-Free Primary-Amine-Specific Bioconjugation Reagents for Peptides, Proteins and Lipids
Linear Mode
