Automated protein resonance assignments of magic angle spinning solid-state NMR spect

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Automated protein resonance assignments of magic angle spinning solid-state NMR spectra of β1 immunoglobulin binding domain of protein G (GB1)


Abstract Magic-angle spinning solid-state NMR (MAS SSNMR) represents a fast developing experimental technique with great potential to provide structural and dynamics information for proteins not amenable to other methods. However, few automated analysis tools are currently available for MAS SSNMR. We present a methodology for automating protein resonance assignments of MAS SSNMR spectral data and its application to experimental peak lists of the β1 immunoglobulin binding domain of protein G (GB1) derived from a uniformly 13C- and 15N-labeled sample. This application to the 56 amino acid GB1 produced an overall 84.1% assignment of the N, CO, CA, and CB resonances with no errors using peak lists from NCACX 3D, CANcoCA 3D, and CANCOCX 4D experiments. This proof of concept demonstrates the tractability of this problem.

• Content Type Journal Article
• DOI 10.1007/s10858-010-9448-2
• Authors
  • Hunter N. B. Moseley, Department of Chemistry, University of Louisville, 2320 South Brook Street, Louisville, KY 40292, USA
  • Lindsay J. Sperling, Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
  • Chad M. Rienstra, Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA

• • Journal Journal of Biomolecular NMR
  • Online ISSN 1573-5001
  • Print ISSN 0925-2738

Source: Journal of Biomolecular NMR