NMR paper Assignment of amide proton signals by combined evaluation of HN, NN and HNCA MAS-NMR

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[NMR paper] Assignment of amide proton signals by combined evaluation of HN, NN and HNCA MAS-NMR

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11-24-2010, 09:01 PM

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Assignment of amide proton signals by combined evaluation of HN, NN and HNCA MAS-NMR

Assignment of amide proton signals by combined evaluation of HN, NN and HNCA MAS-NMR correlation spectra.

Related Articles Assignment of amide proton signals by combined evaluation of HN, NN and HNCA MAS-NMR correlation spectra.

J Biomol NMR. 2003 Mar;25(3):217-23

Authors: van Rossum BJ, Castellani F, Pauli J, Rehbein K, Hollander J, de Groot HJ, Oschkinat H

In this paper, we present a strategy for the (1)H(N) resonance assignment in solid-state magic-angle spinning (MAS) NMR, using the alpha-spectrin SH3 domain as an example. A novel 3D triple resonance experiment is presented that yields intraresidue H(N)-N-C(alpha) correlations, which was essential for the proton assignment. For the observable residues, 52 out of the 54 amide proton resonances were assigned from 2D ((1)H-(15)N) and 3D ((1)H-(15)N-(13)C) heteronuclear correlation spectra. It is demonstrated that proton-driven spin diffusion (PDSD) experiments recorded with long mixing times (4 s) are helpful for confirming the assignment of the protein backbone (15)N resonances and as an aid in the amide proton assignment.

PMID: 12652133 [PubMed - indexed for MEDLINE]

Source: PubMed

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