Related ArticlesAssignment of amide proton signals by combined evaluation of HN, NN and HNCA MAS-NMR correlation spectra.
J Biomol NMR. 2003 Mar;25(3):217-23
Authors: van Rossum BJ, Castellani F, Pauli J, Rehbein K, Hollander J, de Groot HJ, Oschkinat H
In this paper, we present a strategy for the (1)H(N) resonance assignment in solid-state magic-angle spinning (MAS) NMR, using the alpha-spectrin SH3 domain as an example. A novel 3D triple resonance experiment is presented that yields intraresidue H(N)-N-C(alpha) correlations, which was essential for the proton assignment. For the observable residues, 52 out of the 54 amide proton resonances were assigned from 2D ((1)H-(15)N) and 3D ((1)H-(15)N-(13)C) heteronuclear correlation spectra. It is demonstrated that proton-driven spin diffusion (PDSD) experiments recorded with long mixing times (4 s) are helpful for confirming the assignment of the protein backbone (15)N resonances and as an aid in the amide proton assignment.
A simplified recipe for assigning amide NMR signals using combinatorial (14)N amino acid inverse-labeling.
A simplified recipe for assigning amide NMR signals using combinatorial (14)N amino acid inverse-labeling.
A simplified recipe for assigning amide NMR signals using combinatorial (14)N amino acid inverse-labeling.
J Struct Funct Genomics. 2011 Aug 25;
Authors: Hiroaki H, Umetsu Y, Nabeshima YI, Hoshi M, Kohda D
Abstract
Assignment of backbone amide proton resonances is one of the most time-consuming stages of any protein NMR study when the protein samples behave non-ideally. A robust and convenient NMR procedure for analyzing spectra of...
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08-26-2011 04:22 PM
[NMR paper] Proton NMR visible mobile lipid signals in sensitive and multidrug-resistant K562 cel
Proton NMR visible mobile lipid signals in sensitive and multidrug-resistant K562 cells are modulated by rafts.
Related Articles Proton NMR visible mobile lipid signals in sensitive and multidrug-resistant K562 cells are modulated by rafts.
Cancer Cell Int. 2005 Feb 9;5(1):2
Authors: Mannechez A, Reungpatthanaphong P, de Certaines JD, Leray G, Le Moyec L
BACKGROUND: Most cancer cells are characterized by mobile lipids visible on proton NMR (1H-NMR), these being comprised mainly of methyl and methylene signals from lipid acyl chains....
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[NMR paper] 3D TROSY-HNCA(coded)CB and TROSY-HNCA(coded)CO experiments: triple resonance NMR expe
3D TROSY-HNCA(coded)CB and TROSY-HNCA(coded)CO experiments: triple resonance NMR experiments with two sequential connectivity pathways and high sensitivity.
Related Articles 3D TROSY-HNCA(coded)CB and TROSY-HNCA(coded)CO experiments: triple resonance NMR experiments with two sequential connectivity pathways and high sensitivity.
J Biomol NMR. 2004 Mar;28(3):289-94
Authors: Ritter C, Lührs T, Kwiatkowski W, Riek R
The concept of chemical shift-coding monitors chemical shifts in multi-dimensional NMR experiments without additional polarization...
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11-24-2010 09:25 PM
[NMR paper] Evaluation of backbone proton positions and dynamics in a small protein by liquid cry
Evaluation of backbone proton positions and dynamics in a small protein by liquid crystal NMR spectroscopy.
Related Articles Evaluation of backbone proton positions and dynamics in a small protein by liquid crystal NMR spectroscopy.
J Am Chem Soc. 2003 Jul 30;125(30):9179-91
Authors: Ulmer TS, Ramirez BE, Delaglio F, Bax A
NMR measurements of a large set of protein backbone one-bond dipolar couplings have been carried out to refine the structure of the third IgG-binding domain of Protein G (GB3), previously solved by X-ray crystallography at a...
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[NMR paper] A sequential HNCA NMR pulse sequence for protein backbone assignment.
A sequential HNCA NMR pulse sequence for protein backbone assignment.
Related Articles A sequential HNCA NMR pulse sequence for protein backbone assignment.
J Magn Reson. 2001 May;150(1):100-4
Authors: Meissner A, Sĝrensen OW
The conventional HNCA pulse sequence suffers from the ambiguity that it cannot distinguish inter- and intraresidue correlations because the one-bond and two-bond J(NC(alpha)) coupling constants are of similar magnitude. This paper presents a novel pulse sequence, sequential HNCA, that leads to a spectrum exhibiting...
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11-19-2010 08:32 PM
[NMR paper] Assignment of 1H, 13C and 15N NMR signals in the toluene 4-monooxygenase effector pro
Assignment of 1H, 13C and 15N NMR signals in the toluene 4-monooxygenase effector protein.
Related Articles Assignment of 1H, 13C and 15N NMR signals in the toluene 4-monooxygenase effector protein.
J Biomol NMR. 2000 Apr;16(4):359-60
Authors: Hemmi H, Studts JM, Chae YK, Markley JL, Fox BG
Assignment of amide proton signals by combined evaluation of HN, NN and HNCA MAS-NMR
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