Monoclonal antibodies (mAbs) represent the largest class of therapeutic protein drug products. mAb glycosylation produces a heterogeneous, analytically challenging distribution of glycoforms that typically should be adequately characterized because glycosylation-based product quality attributes (PQAs) can impact product quality, immunogenicity, and efficacy. In this study, two products were compared using a panel of analytical methods. Two high-resolution mass spectrometry (HRMS) workflows were...
[NMR paper] Solid-State NMR Characterization of Lyophilized Formulations of Monoclonal Antibody Therapeutics
Solid-State NMR Characterization of Lyophilized Formulations of Monoclonal Antibody Therapeutics
Monoclonal antibodies (mAbs) are an important and growing class of biotherapeutic drugs. Method development for the characterization of critical quality attributes, including higher-order structure (HOS), of mAbs remains an area of active inquiry. Recently, solution-state nuclear magnetic resonance (NMR) spectroscopy has received increased attention and is a means for reliable, high-resolution HOS characterization of aqueous-based preparations of mAbs. While mAbs are predominantly formulated...
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[NMR paper] Assessment of the Higher-Order Structure of Formulated Monoclonal Antibody Therapeutics by 2D Methyl Correlated NMR and Principal Component Analysis.
Assessment of the Higher-Order Structure of Formulated Monoclonal Antibody Therapeutics by 2D Methyl Correlated NMR and Principal Component Analysis.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-7388-69-wiley-full-text.png Assessment of the Higher-Order Structure of Formulated Monoclonal Antibody Therapeutics by 2D Methyl Correlated NMR and Principal Component Analysis.
Curr Protoc Protein Sci. 2020 Jun;100(1):e105
Authors: Arbogast LW, Delaglio F, Brinson RG, Marino JP
Abstract
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[NMR paper] Comparative Analysis of 1D PROFILE and 2D 1H,13C Methyl NMR Methods for Characterization of the Higher Order Structure of IgG1 Monoclonal Antibodies.
Comparative Analysis of 1D PROFILE and 2D 1H,13C Methyl NMR Methods for Characterization of the Higher Order Structure of IgG1 Monoclonal Antibodies.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Comparative Analysis of 1D PROFILE and 2D 1H,13C Methyl NMR Methods for Characterization of the Higher Order Structure of IgG1 Monoclonal Antibodies.
Anal Chem. 2020 Apr 08;:
Authors: Elliott KW, Ghasriani H, Wikstrom M, Giddens JP, Aubin Y, Delaglio F, Marino JP, Arbogast L
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[NMR paper] Structural insights into the interaction of a monoclonal antibody and Nodal peptides by STD-NMR spectroscopy.
Structural insights into the interaction of a monoclonal antibody and Nodal peptides by STD-NMR spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Structural insights into the interaction of a monoclonal antibody and Nodal peptides by STD-NMR spectroscopy.
Bioorg Med Chem. 2017 Dec 15;25(24):6589-6596
Authors: Calvanese L, Focà A, Sandomenico A, Focà G, Caporale A, Doti N, Iaccarino E, Leonardi A, D'Auria G, Ruvo M, Falcigno L
Abstract
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[NMR paper] Characterizing monoclonal antibody formulations in arginine glutamate solutions using (1)H NMR spectroscopy.
Characterizing monoclonal antibody formulations in arginine glutamate solutions using (1)H NMR spectroscopy.
Related Articles Characterizing monoclonal antibody formulations in arginine glutamate solutions using (1)H NMR spectroscopy.
MAbs. 2016 Aug 11;:1-14
Authors: Kheddo P, Cliff MJ, Uddin S, van der Walle CF, Golovanov AP
Abstract
Assessing how excipients affect the self-association of monoclonal antibodies (mAbs) requires informative and direct in situ measurements for highly concentrated solutions, without sample dilution...
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09-03-2016 05:38 PM
[NMR paper] Mapping monoclonal antibody structure by 2D 13C NMR at natural abundance.
Mapping monoclonal antibody structure by 2D 13C NMR at natural abundance.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Mapping monoclonal antibody structure by 2D 13C NMR at natural abundance.
Anal Chem. 2015 Apr 7;87(7):3556-61
Authors: Arbogast LW, Brinson RG, Marino JP
Abstract
Monoclonal antibodies (mAbs) represent an important and rapidly growing class of biotherapeutics. Correct folding of a mAb is critical for drug efficacy, while misfolding...
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09-19-2015 02:53 PM
[NMR paper] Unambiguous characterization of N-glycans of monoclonal antibody cetuximab by integration of LC-MS/MS and ¹H NMR spectroscopy.
Unambiguous characterization of N-glycans of monoclonal antibody cetuximab by integration of LC-MS/MS and ¹H NMR spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Unambiguous characterization of N-glycans of monoclonal antibody cetuximab by integration of LC-MS/MS and ¹H NMR spectroscopy.
Anal Chem. 2014 May 20;86(10):4807-14
Authors: Wiegandt A, Meyer B
Abstract
Monoclonal antibodies are most rapidly emerging as therapeutic drugs for...
Assessment of monoclonal antibody glycosylation: a comparative study using HRMS, NMR, and HILIC-FLD
Linear Mode
