Related ArticlesArginine interactions with anatase TiO(2) (100) surface and the perturbation of (49)Ti NMR chemical shifts - a DFT investigation: relevance to Renu-Seeram bio solar cell.
J Mol Model. 2010 Sep 21;
Authors: Koch R, Lipton AS, Filipek S, Renugopalakrishnan V
Density functional theoretical calculations have been utilized to investigate the interaction of the amino acid arginine with the (100) surface of anatase and the reproduction of experimentally measured (49)Ti NMR chemical shifts of anatase. Significant binding of arginine through electrostatic interaction and hydrogen bonds of the arginine guanidinium protons to the TiO(2) surface oxygen atoms is observed, allowing attachment of proteins to titania surfaces in the construction of bio-sensitized solar cells. GIAO-B3LYP/6-31G(d) NMR calculation of a three-layer model based on the experimental structure of this TiO(2) modification gives an excellent reproduction of the experimental value (-927 ppm) within +/- 7 ppm, however, the change in relative chemical shifts, EFGs and CSA suggest that the effect of the electrostatic arginine binding might be too small for experimental detection.
PMID: 20853182 [PubMed - as supplied by publisher]
[Question from NMRWiki Q&A forum] HSQC perturbation
HSQC perturbation
Dear All,
I am trying to determine the interaction between 2 proteins. Is there any reference or literature which report the determination of binding constant between 2 proteins in which intensity drop of HSQC cross peaks can be used as the measuring parameter. As when I titrate these 2 proteins only change which I observe is intensity drop. I tried other biophysical techniques to determine binding constant, but results are not satisfactory.
Any suggestion would be useful
Regards
nmrlearner
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