Many RNA architectures were discovered to be involved in a wide range of essential biological processes in all organisms from carrying genetic information to gene expression regulation. The remarkable ability of RNAs to adopt various architectures depending on their environment enables the achievement of their myriads of biological functions. Nuclear Magnetic Resonance (NMR) is a powerful technique to investigate both their structure and dynamics. NMR is also a key tool for studying interactions...
A tailored multi-frequency EPR approach to accurately determine the magnetic resonance parameters of dynamic nuclear polarization agents: application to AMUPol #DNPNMR
From The DNP-NMR Blog:
A tailored multi-frequency EPR approach to accurately determine the magnetic resonance parameters of dynamic nuclear polarization agents: application to AMUPol #DNPNMR
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This is a very nice article illustrating the importance of understanding the EPR parameters of a polarizing agent used in DNP-NMR spectroscopy. Here the 9, 95 and 275 GHz EPR spectroscopy is used to characterize AMUPol and predict its performance in high-field DNP.
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06-05-2017 03:59 PM
[NMR paper] Characterization of G-Quadruplex/Hairpin Transitions of RNAs by (19) F NMR Spectroscopy.
Characterization of G-Quadruplex/Hairpin Transitions of RNAs by (19) F NMR Spectroscopy.
Related Articles Characterization of G-Quadruplex/Hairpin Transitions of RNAs by (19) F NMR Spectroscopy.
Chemistry. 2016 Sep 7;
Authors: Granqvist L, Virta P
Abstract
2'-O- reporter groups have been incorporated into guanosine-rich RNA models (including a known bistable Qd/Hp RNA and two G-rich regions of mRNA of human prion protein, PrP) and applied for the (19) F NMR spectroscopic characterization of plausible G-quadruplex/hairpin (Qd/Hp)...
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09-22-2016 06:31 AM
Using FTIR Spectroscopy to Determine Protein Secondary Structure - News-Medical.net
Using FTIR Spectroscopy to Determine Protein Secondary Structure - News-Medical.net
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Using FTIR Spectroscopy to Determine Protein Secondary Structure
News-Medical.net
Another high-resolution method is nuclear magnetic resonance (NMR) spectroscopy, which can be used to characterize protein structure in liquids. However, this method is only practical when studying small proteins, measuring 15 to 25kDa. Furthermore ...
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06-21-2016 01:09 AM
19 F-labeling of the adenine H2-site to study large RNAs by NMR spectroscopy
19 F-labeling of the adenine H2-site to study large RNAs by NMR spectroscopy
Abstract
In comparison to proteins and protein complexes, the size of RNA amenable to NMR studies is limited despite the development of new isotopic labeling strategies including deuteration and ligation of differentially labeled RNAs. Due to the restricted chemical shift dispersion in only four different nucleotides spectral resolution remains limited in larger RNAs. Labeling RNAs with the NMR-active nucleus 19F has previously been introduced for small RNAs up to 40...
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12-28-2015 12:26 AM
[NMR paper] (19)F-labeling of the adenine H2-site to study large RNAs by NMR spectroscopy.
(19)F-labeling of the adenine H2-site to study large RNAs by NMR spectroscopy.
Related Articles (19)F-labeling of the adenine H2-site to study large RNAs by NMR spectroscopy.
J Biomol NMR. 2015 Dec 24;
Authors: Sochor F, Silvers R, Müller D, Richter C, Fürtig B, Schwalbe H
Abstract
In comparison to proteins and protein complexes, the size of RNA amenable to NMR studies is limited despite the development of new isotopic labeling strategies including deuteration and ligation of differentially labeled RNAs. Due to the restricted...
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12-28-2015 12:26 AM
Stable isotope labeling strategy based on coding theory
Stable isotope labeling strategy based on coding theory
Abstract
We describe a strategy for stable isotope-aided protein nuclear magnetic resonance (NMR) analysis, called stable isotope encoding. The basic idea of this strategy is that amino-acid selective labeling can be considered as â??encoding and decodingâ?? processes, in which the information of amino acid type is encoded by the stable isotope labeling ratio of the corresponding residue and it is decoded by analyzing NMR spectra. According to the idea, the strategy can diminish the...
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08-21-2015 09:52 AM
[NMR paper] Combining NMR and EPR to Determine Structures of Large RNAs and Protein-RNA Complexes in Solution.
Combining NMR and EPR to Determine Structures of Large RNAs and Protein-RNA Complexes in Solution.
Related Articles Combining NMR and EPR to Determine Structures of Large RNAs and Protein-RNA Complexes in Solution.
Methods Enzymol. 2015;558:279-331
Authors: Duss O, Yulikov M, Allain FH, Jeschke G
Abstract
Although functional significance of large noncoding RNAs and their complexes with proteins is well recognized, structural information for this class of systems is very scarce. Their inherent flexibility causes problems in...
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06-13-2015 11:09 PM
[NMR paper] Use of a combination of the RDC method and NOESY NMR spectroscopy to determine the structure of Alzheimer's amyloid A?10-35 peptide in solution and in SDS micelles.
Use of a combination of the RDC method and NOESY NMR spectroscopy to determine the structure of Alzheimer's amyloid A?10-35 peptide in solution and in SDS micelles.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles Use of a combination of the RDC method and NOESY NMR spectroscopy to determine the structure of Alzheimer's amyloid A?10-35 peptide in solution and in SDS micelles.
Eur Biophys J. 2013 Dec;42(11-12):803-10
Authors: Usachev KS, Filippov AV,...
Application of NMR Spectroscopy to Determine the 3D Structure of Small Non-Coding RNAs
Linear Mode
