Small molecules that bind to oligomeric protein species such as membrane proteins and fibrils are of clinical interest for development of therapeutics and diagnostics. Definition of the binding site at atomic resolution via NMR is often challenging due to low binding stoichiometry of the small molecule. For fibrils and aggregation intermediates grown in the presence of lipids, we report atomic-resolution contacts to the small molecule at sub nm distance via solid-state NMR using dynamic nuclear...
[NMR paper] Evolution of ?-synuclein conformation ensemble toward amyloid fibril via liquid-liquid phase separation (LLPS) as investigated by dynamic nuclear polarization-enhanced solid-state MAS NMR
Evolution of ?-synuclein conformation ensemble toward amyloid fibril via liquid-liquid phase separation (LLPS) as investigated by dynamic nuclear polarization-enhanced solid-state MAS NMR
Protein fibrillation and human neurodegenerative diseases, with a profound underlying connection suggested between them, have been the subject of intense investigations in the medical, biophysical and bio-engineering sciences. For gaining the molecular mechanistic insights into such connection, i.e., the cause and effect, atomic-resolution molecular structure information especially on the initial...
Electron decoupling with cross polarization and dynamic nuclear polarization below 6 K #DNPNMR
From The DNP-NMR Blog:
Electron decoupling with cross polarization and dynamic nuclear polarization below 6 K #DNPNMR
Sesti, Erika L., Edward P. Saliba, Nicholas Alaniva, and Alexander B. Barnes. “Electron Decoupling with Cross Polarization and Dynamic Nuclear Polarization below 6 K.” Journal of Magnetic Resonance 295 (October 2018): 1–5.
https://doi.org/10.1016/j.jmr.2018.07.016.
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03-24-2019 10:41 PM
Hyperpolarization of nitrogen-15 nuclei by cross polarization and dissolution dynamic nuclear polarization
From The DNP-NMR Blog:
Hyperpolarization of nitrogen-15 nuclei by cross polarization and dissolution dynamic nuclear polarization
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Milani, J., et al., Hyperpolarization of nitrogen-15 nuclei by cross polarization and dissolution dynamic nuclear polarization. Review of Scientific Instruments, 2017. 88(1): p. 015109.
http://dx.doi.org/10.1063/1.4973777
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09-04-2017 03:11 PM
Dynamic Nuclear Polarization enhanced NMR at 187GHz/284MHz using an Extended Interaction Klystron amplifier #DNPNMR
From The DNP-NMR Blog:
Dynamic Nuclear Polarization enhanced NMR at 187GHz/284MHz using an Extended Interaction Klystron amplifier #DNPNMR
Kemp, T.F., et al., Dynamic Nuclear Polarization enhanced NMR at 187GHz/284MHz using an Extended Interaction Klystron amplifier. J Magn Reson, 2016. 265: p. 77-82.
http://www.ncbi.nlm.nih.gov/pubmed/26867091
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05-18-2016 09:53 PM
PRESTO polarization transfer to quadrupolar nuclei: implications for dynamic nuclear polarization
From The DNP-NMR Blog:
PRESTO polarization transfer to quadrupolar nuclei: implications for dynamic nuclear polarization
Perras, F.A., T. Kobayashi, and M. Pruski, PRESTO polarization transfer to quadrupolar nuclei: implications for dynamic nuclear polarization. Phys Chem Chem Phys, 2015. 17(35): p. 22616-22.
http://www.ncbi.nlm.nih.gov/pubmed/26266874
The Role of the Interaction Frame in the Theoretical Description of Solid Effect Dynamic Nuclear Polarization
From The DNP-NMR Blog:
The Role of the Interaction Frame in the Theoretical Description of Solid Effect Dynamic Nuclear Polarization
Kwiatkowski, G., A. Karabanov, and W. Köckenberger, The Role of the Interaction Frame in the Theoretical Description of Solid Effect Dynamic Nuclear Polarization. Israel Journal of Chemistry, 2014. 54(1-2): p. 184-195.
http://dx.doi.org/10.1002/ijch.201300125
Anle138b interaction in ?-synuclein aggregates by dynamic nuclear polarization NMR
Linear Mode
