Parallel acquisition of multi-dimensional spectra in protein NMR
Parallel acquisition of multi-dimensional spectra in protein NMR
Abstract We introduce the use of multiple receivers applied in parallel for simultaneously recording multi-dimensional data sets of proteins in a single experiment. The utility of the approach is established through the introduction of the 2D 15N,1HN||13CO HSQC experiment in which a pair of two-dimensional 15N,1HN and 15N,13CO spectra are recorded. The methodology is further extended to higher dimensionality via the 3D 1HN||13CO HNCA in which a pair of data sets recording 13Cα,15N,1HN and 13Cα,15N,13CO chemical shifts...
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07-20-2012 11:13 PM
4D Non-uniformly sampled HCBCACON and 1J(NCα)-selective HCBCANCO experiments for the sequential assignment and chemical shift analysis of intrinsically disordered proteins
4D Non-uniformly sampled HCBCACON and 1J(NCα)-selective HCBCANCO experiments for the sequential assignment and chemical shift analysis of intrinsically disordered proteins
Abstract A pair of 4D NMR experiments for the backbone assignment of disordered proteins is presented. The experiments exploit 13C direct detection and non-uniform sampling of the indirectly detected dimensions, and provide correlations of the aliphatic proton (Hα, and Hβ) and carbon (Cα, Cβ) resonance frequencies to the protein backbone. Thus, all the chemical shifts regularly used to map the transient...
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05-17-2012 08:40 AM
Analysis of non-uniformly sampled spectra with multi-dimensional decomposition
Analysis of non-uniformly sampled spectra with multi-dimensional decomposition
Publication year: 2011
Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 59, Issue 3</br>
Vladislav Yu. Orekhov, Victor A. Jaravine</br>
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03-09-2012 09:16 AM
Application of iterative soft thresholding for fast reconstruction of NMR data non-uniformly sampled with multidimensional Poisson Gap scheduling
Application of iterative soft thresholding for fast reconstruction of NMR data non-uniformly sampled with multidimensional Poisson Gap scheduling
Abstract The fast Fourier transformation has been the gold standard for transforming data from time to frequency domain in many spectroscopic methods, including NMR. While reliable, it has as a drawback that it requires a grid of uniformly sampled data points. This needs very long measuring times for sampling in multidimensional experiments in all indirect dimensions uniformly and even does not allow reaching optimal evolution times that would...
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02-16-2012 05:24 AM
Analysis of non-uniformly sampled spectra with Multi-Dimensional Decomposition
Analysis of non-uniformly sampled spectra with Multi-Dimensional Decomposition
Publication year: 2011
Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 24 February 2011</br>
Vladislav Yu., Orekhov , Victor A., Jaravine</br>
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02-26-2011 01:07 PM
FM reconstruction of non-uniformly sampled protein NMR data at higher dimensions and optimization by distillation
FM reconstruction of non-uniformly sampled protein NMR data at higher dimensions and optimization by distillation
Abstract Non-uniform sampling (NUS) enables recording of multidimensional NMR data at resolutions matching the resolving power of modern instruments without using excessive measuring time. However, in order to obtain satisfying results, efficient reconstruction methods are needed. Here we describe an optimized version of the Forward Maximum entropy (FM) reconstruction method, which can reconstruct up to three indirect dimensions. For complex datasets, such as NOESY spectra,...
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01-09-2011 12:46 PM
PRODECOMP - program for decomposition of NMR spectra with coupled evolution periods
Multiway Decomposition of NMR Spectra with Coupled Evolution Periods
Daniel Malmodin and Martin Billeter
J. Am. Chem. Soc.; 2005; 127(39), pp 13486 - 13487
http://pubs.acs.org/isubscribe/journals/jacsat/127/i39/figures/ja0545822n00001.gif
Abstract:
Coupling evolution periods in NMR experiments on proteins has recently attracted much attention for its substantial savings in measurement time. Using the concept of multiway decomposition, which already proved useful in many types of NMR applications, the novel tool PRODECOMP decomposes sets of spectra with coupled evolution periods...
Analysis of non-uniformly sampled spectra with multi-dimensional decomposition
Linear Mode
