?-Methylacyl-CoA racemase (AMACR; P504S) catalyzes the conversion of R-2-methylacyl-CoA esters into their corresponding S-2-methylacyl-CoA epimers enabling their degradation by ?-oxidation. The enzyme also catalyzes the key epimerization reaction in the pharmacological activation pathway of ibuprofen and related drugs. AMACR protein levels and enzymatic activity are increased in prostate cancer, and the enzyme is a recognized drug target. Key to the development of novel treatments based on AMACR...
[NMR paper] Glycan analysis: scope and limitations of different techniques--a case for integrated use of LC-MS(/MS) and NMR techniques.
Glycan analysis: scope and limitations of different techniques--a case for integrated use of LC-MS(/MS) and NMR techniques.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles Glycan analysis: scope and limitations of different techniques--a case for integrated use of LC-MS(/MS) and NMR techniques.
Anal Bioanal Chem. 2013 Sep;405(23):7291-305
Authors: Fellenberg M, Behnken HN, Nagel T, Wiegandt A, Baerenfaenger M, Meyer B
Abstract
...
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Applications of dynamic nuclear polarization to the study of reactions and reagents in organic and biomolecular chemistry
From The DNP-NMR Blog:
Applications of dynamic nuclear polarization to the study of reactions and reagents in organic and biomolecular chemistry
Hilty, C. and S. Bowen, Applications of dynamic nuclear polarization to the study of reactions and reagents in organic and biomolecular chemistry. Organic & Biomolecular Chemistry, 2010. 8(15): p. 3361-3365.
http://dx.doi.org/10.1039/C004420M
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05-17-2013 07:00 PM
Analysis of sensitivity enhancement by dynamic nuclear polarization in solid-state NMR: a case study of functionalized mesoporous materials
From The DNP-NMR Blog:
Analysis of sensitivity enhancement by dynamic nuclear polarization in solid-state NMR: a case study of functionalized mesoporous materials
Kobayashi, T., et al., Analysis of sensitivity enhancement by dynamic nuclear polarization in solid-state NMR: a case study of functionalized mesoporous materials. Phys Chem Chem Phys, 2013. 15(15): p. 5553-62.
http://www.ncbi.nlm.nih.gov/pubmed/23459985
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04-17-2013 08:15 PM
Analysis of sensitivity enhancement by dynamic nuclear polarization in solid-state NMR: a case study of functionalized mesoporous materials
From the The DNP-NMR Blog:
Analysis of sensitivity enhancement by dynamic nuclear polarization in solid-state NMR: a case study of functionalized mesoporous materials
Kobayashi, T., et al., Analysis of sensitivity enhancement by dynamic nuclear polarization in solid-state NMR: a case study of functionalized mesoporous materials. Phys Chem Chem Phys, 2013. 15(15): p. 5553-62.
http://www.ncbi.nlm.nih.gov/pubmed/23459985
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04-15-2013 08:52 AM
[NMR paper] NMR for direct determination of K(m) and V(max) of enzyme reactions based on the Lambert W function-analysis of progress curves.
NMR for direct determination of K(m) and V(max) of enzyme reactions based on the Lambert W function-analysis of progress curves.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles NMR for direct determination of K(m) and V(max) of enzyme reactions based on the Lambert W function-analysis of progress curves.
Biochim Biophys Acta. 2012 Mar;1824(3):443-9
Authors: Exnowitz F, Meyer B, Hackl T
Abstract
(1)H NMR spectroscopy was used to follow the...
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04-03-2013 08:22 PM
[NMR paper] A novel microfluidic rapid freeze-quench device for trapping reactions intermediates for high field EPR analysis
A novel microfluidic rapid freeze-quench device for trapping reactions intermediates for high field EPR analysis
Available online 5 March 2013
Publication year: 2013
Source:Journal of Magnetic Resonance</br>
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Rapid freeze quench electron paramagnetic resonance (RFQ)-EPR is a method for trapping short lived intermediates in chemical reactions and subjecting them to EPR spectroscopy investigation for their characterization. Two (or more) reacting components are mixed at room temperature and after some delay the mixture is sprayed into a cold trap and transferred into...
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[NMR paper] A systematic case study on using NMR models for molecular replacement: p53 tetrameriz
A systematic case study on using NMR models for molecular replacement: p53 tetramerization domain revisited.
Related Articles A systematic case study on using NMR models for molecular replacement: p53 tetramerization domain revisited.
Acta Crystallogr D Biol Crystallogr. 2000 Dec;56(Pt 12):1535-40
Authors: Chen YW, Clore GM
Molecular replacement using search models derived from nuclear magnetic resonance (NMR) spectroscopy has often proved problematic. It has been known for some time that the overall differences in atomic positions (r.m.s.d.)...
Analysis of enzyme reactions using NMR techniques: A case study with ?-methylacyl-CoA racemase (AMACR)
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