Analysis of the Effects of ?-Tocopherol (?-TOH) on RAW264.7 and K562 Cells Based on 1H-NMR Metabonomics.
J Agric Food Chem. 2018 Jan 09;:
Authors: Lu Y, Li H, Geng Y
Abstract
?-Tocopherol (?-TOH) is a form of vitamin E with higher bioactivity. In this study, we studied the bioactivity of ?-TOH using the IC50 of ?-TOH on RAW264.7 (80 ?M) and K562 (110 ?M) cells. We compared the differential metabolites from the cell lines with and without ?-TOH treatment by 1H-NMR metabonomics analysis. It was found that ?-TOH affected the protein biosynthesis, betaine metabolism, and urea cycle in various ways in both cell lines. Metabolic levels of the cell lines were changed after treatment with ?-TOH as differential metabolites were produced. The betaine level in RAW264.7 cells was reduced significantly while the L-lactic acid level in K562 cells was significantly enhanced. The metabolic changes might contribute to the switch of the respiration pattern from aerobic respiration to anaerobic respiration in K562 cell. These results are helpful in a further understanding of the sub-toxicity of ?-TOH.
PMID: 29313349 [PubMed - as supplied by publisher]
[NMR paper] Effects of doxorubicin, cisplatin, and tamoxifen on the metabolic profile of human breast cancer MCF-7 cells as determined by 1H HR-MAS NMR.
Effects of doxorubicin, cisplatin, and tamoxifen on the metabolic profile of human breast cancer MCF-7 cells as determined by 1H HR-MAS NMR.
Effects of doxorubicin, cisplatin, and tamoxifen on the metabolic profile of human breast cancer MCF-7 cells as determined by 1H HR-MAS NMR.
Biochemistry. 2017 Apr 05;:
Authors: Maria RM, Altei WF, Selistre-de-Araújo HS, Colnago LA
Abstract
Doxorubicin, cisplatin, and tamoxifen are part of many chemotherapeutic regimens. However, there are limited studies investigating the way...
nmrlearner
Journal club
0
04-06-2017 12:16 PM
[NMR paper] Hepatitis B virus X protein (HBx)-induced abnormalities of nucleic acid metabolism revealed by (1)H-NMR-based metabonomics.
Hepatitis B virus X protein (HBx)-induced abnormalities of nucleic acid metabolism revealed by (1)H-NMR-based metabonomics.
Hepatitis B virus X protein (HBx)-induced abnormalities of nucleic acid metabolism revealed by (1)H-NMR-based metabonomics.
Sci Rep. 2016;6:24430
Authors: Dan Yue, Zhang Y, Cheng L, Ma J, Xi Y, Yang L, Su C, Shao B, Huang A, Xiang R, Cheng P
Abstract
Hepatitis B virus X protein (HBx) plays an important role in HBV-related hepatocarcinogenesis; however, mechanisms underlying HBx-mediated carcinogenesis...
[NMR paper] The effects of acute lipopolysaccharide challenge on dairy goat liver metabolism assessed with (1) HNMR metabonomics.
The effects of acute lipopolysaccharide challenge on dairy goat liver metabolism assessed with (1) HNMR metabonomics.
Related Articles The effects of acute lipopolysaccharide challenge on dairy goat liver metabolism assessed with (1) HNMR metabonomics.
J Anim Physiol Anim Nutr (Berl). 2016 Feb 5;
Authors: Wang LF, Jia SD, Yang GQ, Liu RY, Yang GY, Li M, Zhu HS, Wang YY, Han LQ
Abstract
[NMR paper] Studies of single-walled carbon nanotube-induced hepatotoxicity by NMR-based metabonomics of rat blood plasma and liver extracts.
Studies of single-walled carbon nanotube-induced hepatotoxicity by NMR-based metabonomics of rat blood plasma and liver extracts.
Studies of single-walled carbon nanotube-induced hepatotoxicity by NMR-based metabonomics of rat blood plasma and liver extracts.
Nanoscale Res Lett. 2013 May 16;8(1):236
Authors: Lin B, Zhang H, Lin Z, Fang Y, Tian L, Yang H, Yan J, Liu H, Zhang W, Xi Z
Abstract
The toxicological effects of single-walled carbon nanotubes (SWCNTs) were investigated after intratracheal instillation in male Wistar rats over...
nmrlearner
Journal club
0
05-18-2013 09:42 PM
[NMR paper] Proton NMR visible mobile lipid signals in sensitive and multidrug-resistant K562 cel
Proton NMR visible mobile lipid signals in sensitive and multidrug-resistant K562 cells are modulated by rafts.
Related Articles Proton NMR visible mobile lipid signals in sensitive and multidrug-resistant K562 cells are modulated by rafts.
Cancer Cell Int. 2005 Feb 9;5(1):2
Authors: Mannechez A, Reungpatthanaphong P, de Certaines JD, Leray G, Le Moyec L
BACKGROUND: Most cancer cells are characterized by mobile lipids visible on proton NMR (1H-NMR), these being comprised mainly of methyl and methylene signals from lipid acyl chains....
Analysis of the Effects of ?-Tocopherol (?-TOH) on RAW264.7 and K562 Cells Based on 1H-NMR Metabonomics.
Linear Mode
