[NMR paper] Proton-Detected NMR Spectroscopy of Nanodisc-Embedded Membrane Proteins: MAS Solid-State vs. Solution-State Methods.
Proton-Detected NMR Spectroscopy of Nanodisc-Embedded Membrane Proteins: MAS Solid-State vs. Solution-State Methods.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Proton-Detected NMR Spectroscopy of Nanodisc-Embedded Membrane Proteins: MAS Solid-State vs. Solution-State Methods.
J Phys Chem B. 2017 Jul 24;:
Authors: Lakomek NA, Frey L, Bibow S, Böckmann A, Riek R, Meier BH
Abstract
The structural and dynamical characterization of membrane proteins...
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07-25-2017 07:46 PM
[NMR paper] Solubilization of Membrane Proteins into Functional Lipid-Bilayer Nanodiscs Using a Diisobutylene/Maleic Acid Copolymer
Solubilization of Membrane Proteins into Functional Lipid-Bilayer Nanodiscs Using a Diisobutylene/Maleic Acid Copolymer
Once removed from their natural environment, membrane proteins depend on membrane-mimetic systems to retain their native structures and functions. To this end, lipid-bilayer nanodiscs that are bounded by scaffold proteins or amphiphilic polymers such as styrene/maleic acid (SMA) copolymers have been introduced as alternatives to detergent micelles and liposomes for in vitro membrane-protein research. Herein, we show that an alternating diisobutylene/maleic acid...
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01-12-2017 05:31 PM
[NMR paper] The power, pitfalls and potential of the nanodisc system for NMR-based studies.
The power, pitfalls and potential of the nanodisc system for NMR-based studies.
Related Articles The power, pitfalls and potential of the nanodisc system for NMR-based studies.
Biol Chem. 2016 Jul 23;
Authors: Viegas A, Viennet T, Etzkorn M
Abstract
The choice of a suitable membrane mimicking environment is of fundamental importance for the characterization of structure and function of membrane proteins. In this respect, usage of the lipid bilayer nanodisc technology provides a unique potential for NMR-based studies. This review...
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07-28-2016 12:07 PM
[NMR paper] Synthesis of uniformly deuterated n-dodecyl-?-D-maltoside (d39 -DDM) for solubilization of membrane proteins in TROSY NMR experiments.
Synthesis of uniformly deuterated n-dodecyl-?-D-maltoside (d39 -DDM) for solubilization of membrane proteins in TROSY NMR experiments.
Related Articles Synthesis of uniformly deuterated n-dodecyl-?-D-maltoside (d39 -DDM) for solubilization of membrane proteins in TROSY NMR experiments.
J Labelled Comp Radiopharm. 2014 Dec 9;
Authors: Hiruma-Shimizu K, Kalverda AP, Henderson PJ, Homans SW, Patching SG
Abstract
This work reports the first synthesis of uniformly deuterated n-dodecyl-?-D-maltoside (d39 -DDM). DDM is a mild non-ionic...
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12-11-2014 11:22 PM
Membrane-Dependent Modulation of the mTOR ActivatorRheb: NMR Observations of a GTPase Tethered to a Lipid-Bilayer Nanodisc
Membrane-Dependent Modulation of the mTOR ActivatorRheb: NMR Observations of a GTPase Tethered to a Lipid-Bilayer Nanodisc
Mohammad T. Mazhab-Jafari, Christopher B. Marshall, Peter B. Stathopulos, Yoshihiro Kobashigawa, Vuk Stambolic, Lewis E. Kay, Fuyuhiko Inagaki and Mitsuhiko Ikura
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja312508w/aop/images/medium/ja-2012-12508w_0003.gif
Journal of the American Chemical Society
DOI: 10.1021/ja312508w
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02-27-2013 06:45 AM
[NMR paper] Membrane-dependent modulation of the mTOR activator Rheb: NMR observations of a GTPase tethered to a lipid-bilayer nanodisc.
Membrane-dependent modulation of the mTOR activator Rheb: NMR observations of a GTPase tethered to a lipid-bilayer nanodisc.
Membrane-dependent modulation of the mTOR activator Rheb: NMR observations of a GTPase tethered to a lipid-bilayer nanodisc.
J Am Chem Soc. 2013 Feb 14;
Authors: Mazhab-Jafari MT, Marshall CB, Stathopulos PB, Kobashigawa Y, Stambolic V, Kay LE, Inagaki F, Ikura M
Abstract
Like most Ras superfamily proteins, the GTPase domain of Ras homolog enriched in brain (Rheb) is tethered to cellular membranes through a...
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02-16-2013 08:00 PM
[NMR paper] Detergent/Nanodisc screening for high-resolution NMR studies of an integral membrane protein containing a cytoplasmic domain.
Detergent/Nanodisc screening for high-resolution NMR studies of an integral membrane protein containing a cytoplasmic domain.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.plosone.org-images-pone_120x30.png http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Detergent/Nanodisc screening for high-resolution NMR studies of an integral membrane protein containing a cytoplasmic domain.
PLoS One. 2013;8(1):e54378
Authors: Tzitzilonis C, Eichmann C, Maslennikov I, Choe...
Alternatives to Styrene- and Diisobutylene-based Copolymers for Membrane Protein Solubilization via Nanodisc Formation
Linear Mode
