Abstract Sparse sampling in biomolecular multidimensional NMR offers increased acquisition speed and resolution and, if appropriate conditions are met, an increase in sensitivity. Sparse sampling of indirectly detected time domains combined with the direct truly multidimensional Fourier transform has elicited particular attention because of the ability to generate a final spectrum amenable to traditional analysis techniques. A number of sparse sampling schemes have been described including radial sampling, random sampling, concentric sampling and variations thereof. A fundamental feature of these sampling schemes is that the resulting time domain data array is not amenable to traditional Fourier transform based processing and phasing correction techniques. In addition, radial sampling approaches offer a number of advantages and capabilities that are also not accessible using standard NMR processing techniques. These include sensitivity enhancement, sub-matrix processing and determination of minimal sets of sampling angles. Here we describe a new software package (Al NMR) that enables these capabilities in the context of a general NMR data processing environment.
Content Type Journal Article
Category Article
Pages 1-11
DOI 10.1007/s10858-011-9584-3
Authors
John M. Gledhill, Graduate Group in Biochemistry and Molecular Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6059, USA
A. Joshua Wand, Graduate Group in Biochemistry and Molecular Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6059, USA
[Question from NMRWiki Q&A forum] data processing
data processing
HELLO NMR WIKI ERS
Could you please give information about the standred reference values of LINEAR PRIDICTION FORWRAD or BACKWORD during the processing of 3D data NMR DATA and when the sinebell shift value is needed and which wieghting function Shall i use it everytime to get good contours or signal on VNMRJ software
Thanking You in anticipation
Sri
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Publication year: 2010
Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 12 June 2010</br>
Dominique, Marion</br>
Resonance assignment of intrinsically disordered proteins is made difficult by the extensive spectral overlaps. High-resolution 3D and 4D spectra are thus essential for this purpose. We have adapted the series of 3D BEST experiments proposed by Lescop et al to the case of unfolded proteins. Longer acquisitions...
Al NMR: a novel NMR data processing program optimized for sparse sampling
Linear Mode
