The measurement and application of residual dipolar couplings (RDCs) in solution NMR studies of biological macromolecules has become well established over the past quarter of a century. Numerous methods for generating the requisite anisotropic orientational molecular distribution have been demonstrated, each with its specific strengths and weaknesses. In parallel, an enormous number of pulse schemes have been introduced to measure the many different types of RDCs, ranging from the most widely...
[NMR paper] Conformation of inhibitor-free HIV-1 protease derived from NMR spectroscopy in a weakly oriented solution.
Conformation of inhibitor-free HIV-1 protease derived from NMR spectroscopy in a weakly oriented solution.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary_FullTextOnline_120x27.gif Related Articles Conformation of inhibitor-free HIV-1 protease derived from NMR spectroscopy in a weakly oriented solution.
Chembiochem. 2015 Jan 19;16(2):214-8
Authors: Roche J, Louis JM, Bax A
Abstract
Flexibility of the glycine-rich flaps is known to be essential for catalytic...
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[NMR paper] High-resolution NMR spectroscopy of membrane proteins in aligned bicelles.
High-resolution NMR spectroscopy of membrane proteins in aligned bicelles.
Related Articles High-resolution NMR spectroscopy of membrane proteins in aligned bicelles.
J Am Chem Soc. 2004 Dec 1;126(47):15340-1
Authors: De Angelis AA, Nevzorov AA, Park SH, Howell SC, Mrse AA, Opella SJ
High-resolution solid-state NMR spectra can be obtained from uniformly (15)N-labeled membrane proteins in magnetically aligned bicelles. Fast uniaxial diffusion about the axis of the bilayer normal results in single-line spectra that contain the orientational...
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[NMR paper] Structure determination of aligned samples of membrane proteins by NMR spectroscopy.
Structure determination of aligned samples of membrane proteins by NMR spectroscopy.
Related Articles Structure determination of aligned samples of membrane proteins by NMR spectroscopy.
Magn Reson Chem. 2004 Feb;42(2):162-71
Authors: Nevzorov AA, Mesleh MF, Opella SJ
The paper briefly reviews the process of determining the structures of membrane proteins by NMR spectroscopy of aligned samples, describes the integration of recent developments in the interpretation of spectra of aligned proteins and illustrates the application of these methods...
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[NMR paper] An NMR method for studying the kinetics of metal exchange in biomolecular systems.
An NMR method for studying the kinetics of metal exchange in biomolecular systems.
Related Articles An NMR method for studying the kinetics of metal exchange in biomolecular systems.
J Biomol NMR. 2002 Aug;23(4):303-9
Authors: Barbieri R, Hore PJ, Luchina C, Pierattelli R
The kinetics of lanthanide (III) exchange for calcium(II) in the C-terminal EF-hand of the protein calbindin D9k have been studied by one-dimensional (1D) stopped-flow NMR. By choosing a paramagnetic lanthanide (Ce3+), kinetics in the sub-second range can be easily measured....
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[NMR paper] Theoretical and computational advances in biomolecular NMR spectroscopy.
Theoretical and computational advances in biomolecular NMR spectroscopy.
Related Articles Theoretical and computational advances in biomolecular NMR spectroscopy.
Curr Opin Struct Biol. 2002 Apr;12(2):146-53
Authors: Clore GM, Schwieters CD
Recent developments in experimental and computational aspects of NMR spectroscopy have had a significant impact on the accuracy and speed of macromolecular structure determination in solution, particularly with regard to systems of high complexity (such as protein complexes). These include experiments...
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[NMR paper] Biomolecular solid state NMR: advances in structural methodology and applications to
Biomolecular solid state NMR: advances in structural methodology and applications to peptide and protein fibrils.
Related Articles Biomolecular solid state NMR: advances in structural methodology and applications to peptide and protein fibrils.
Annu Rev Phys Chem. 2001;52:575-606
Authors: Tycko R
Solid state nuclear magnetic resonance (NMR) methods can provide atomic-level structural constraints on peptides and proteins in forms that are not amenable to characterization by other high-resolution structural techniques, owing to insolubility,...
Advances in NMR Spectroscopy of Weakly Aligned Biomolecular Systems
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